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Γιάννης Στρουμπούλης

Γιάννης Στρουμπούλης

Διευθυντής Ερευνών
Ηλ. Ταχυδρομείο
Αυτή η διεύθυνση ηλεκτρονικού ταχυδρομείου προστατεύεται από τους αυτοματισμούς αποστολέων ανεπιθύμητων μηνυμάτων. Χρειάζεται να ενεργοποιήσετε τη JavaScript για να μπορέσετε να τη δείτε.
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Ιστοσελίδα Εργαστηρίου

Η έρευνά μας εστιάζεται στη μεταγραφική και επιγενετική ρύθμιση της αιμοποίησης με επίκεντρο τον λειτουργικό χαρακτηρισμό σημαντικών μεταγραφικών παραγόντων στην διαφοροποίησης των ερυθρών αιμοσφαιρίων, μέσω πρωτεομικών και γονιδιωματικών προσεγγίσεων.

Η έρευνά μας έχει στόχο να κατανοήσει τη μοριακή βάση της δράσης μεταγραφικών ρυθμιστικών παραγόντων στην αιματοποίηση, με επίκεντρο την μελέτη των μεταγραφικών παραγόντων GATA-1 και FOG-1 που παίζουν σημαντικούς ρυθμιστικούς ρόλους στην διαφοροποίηση των ερυθρών αιμοσφαιρίων. Εφαρμόζουμε μεθοδολογία in vivo σήμανσης πρωτεϊνών μέσω βιοτινυλίωσης ως μια αποτελεσματική και υψηλής συγγένειας μέθοδο για την ταχεία απομόνωση και χαρακτηρισμό με φασματομετρία μάζας πρωτεϊνικών συμπλόκων που εμπλέκονται στην μεταγραφική ρύθμιση της ερυθροειδικής διαφοροποίησης, όπως και για την διασαφήνιση των γονιδιακών στόχων που ρυθμίζονται από τα σύμπλοκα αυτά.

Amanatiadou EP, Papadopoulos GL, Strouboulis J,* Vizirianakis IS. (2015) GATA1 and PU.1 Bind to Ribosomal Protein Genes in Erythroid Cells: Implications for Ribosomopathies. PLoS One. 10: e0140077. * Co-corresponding author.

Fan J.  A.X, Papadopoulos G.L, Lin I-J, Hossain M, Hu J, Renne R, Strouboulis J,* Bungert J. (2014) Proteomic and Genomic Analysis of transcription factor TFII-I in erythroleukemia cells. Nucleic Acids Res.  42: 7625-41.  * Co-corresponding author.

Papadopoulos G.L, Karkoulia E, Tsamardinos I, Porcher C, Ragoussis J, Bungert J, Strouboulis J.  (2013) GATA-1 genome-wide occupancy associates with distinct epigenetic profiles during mouse fetal liver erythropoiesis. Nucleic Acids Res. 41: 4938-48.

Rodriguez P, Bonte E, Krijgsveld J, Guyot B, Heck A, Vyas P, de Boer E, Grosveld F, Strouboulis,J. (2005) GATA-1 forms distinct activating and repressive complexes in erythroid cells. EMBO J.  24: 2354–2366.

de Boer Ε, Rodriguez P, Bonte E, Krijgsveld J, Katsantoni E, Heck A, Grosveld F, Strouboulis J. (2003) Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proceedings Nat. Acad. Sci. USA 100: 7480-7485.

2017

Shen Y, Nar R, Fan AX, Aryan M, Hossain MA, Gurumurthy A, Wassel PC, Tang M, Lu J, Strouboulis J, Bungert J. "Functional interrelationship between TFII-I and E2F transcription factors at specific cell cycle gene loci". J Cell Biochem. 2017 Jun 28. doi: 10.1002/jcb.26235. [Epub ahead of print] [PubMed]

Chatzinikolaou G, Apostolou Z, Aid-Pavlidis T, Ioannidou A, Karakasilioti I, Papadopoulos GL, Aivaliotis M, Tsekrekou M, Strouboulis J, Kosteas T, Garinis GA. "ERCC1-XPF cooperates with CTCF and cohesin to facilitate the developmental silencing of imprinted genes". Nat Cell Biol. 2017 May;19(5):421-432. [PubMed]

2016

Papageorgiou DN, Karkoulia E, Amaral-Psarris A, Burda P, Kolodziej K, Demmers J, Bungert J, Stopka T, Strouboulis J. "Distinct and overlapping DNMT1 interactions with multiple transcription factors in erythroid cells: Evidence for co-repressor functions." Biochim Biophys Acta. 2016 Dec;1859(12):1515-1526. [PubMed]

Burda P, Vargova J, Curik N, Salek C, Papadopoulos GL, Strouboulis J, Stopka T. "GATA-1 Inhibits PU.1 Gene via DNA and Histone H3K9 Methylation of Its Distal Enhancer in Erythroleukemia". PLoS One. 2016 Mar 24;11(3):e0152234. [PubMed]

2015

Amanatiadou EP, Papadopoulos GL, Strouboulis J,* Vizirianakis IS. "GATA1 and PU.1 Bind to Ribosomal Protein Genes in Erythroid Cells: Implications for Ribosomopathies". PLoS One. 2015 Oct 8;10(10):e0140077. * Co-corresponding author. [PubMed]

Blobel GA, Bodine D, Brand M, Crispino J, de Bruijn MF, Nathan D, Papayannopoulou T, Porcher C, Strouboulis J, Zon L, Higgs DR, Stamatoyannopoulos G, Engel JD. 2015, "An international effort to cure a global health problem: A report on the 19th Hemoglobin Switching Conference." Exp Hematol. 2015 Oct;43(10):821-37. [PubMed]

Papadopoulos P, Gutiérrez L, Demmers J, Scheer E, Pourfarzad F, Papageorgiou DN, Karkoulia E, Strouboulis J, van de Werken HJ, van der Linden R, Vandenberghe P, Dekkers DH, Philipsen S, Grosveld F, Tora L. 2015, "TAF10 interacts with GATA1 transcription factor and controls mouse erythropoiesis." Mol Cell Biol. 2015 Apr 13. pii: MCB.01370-14. [PubMed]

2014

Fan AX, Papadopoulos GL, Hossain MA, Lin IJ, Hu J, Tang TM, Kilberg MS, Renne R, Strouboulis J*, Bungert J. 2014, "Genomic and proteomic analysis of transcription factor TFII-I reveals insight into the response to cellular stress", Nucleic Acids Res., 2014 2014, 42, pp. 7625-41. * Co-corresponding author [PubMed].

Shi L, Sierant MC, Gurdziel K, Zhu F, Cui S, Kolodziej KE, Strouboulis J, Guan Y, Tanabe O, Lim KC, Engel JD 2014, "Biased, Non-equivalent Gene-Proximal and -Distal Binding Motifs of Orphan Nuclear Receptor TR4 in Primary Human Erythroid Cells.", PLoS Genet., 2014 May 8; 10(5):e1004339. [PubMed].

2013

Papadopoulos GL, Karkoulia E, Tsamardinos I, Porcher C, Ragoussis J, Bungert J, Strouboulis J . 2013, "GATA-1 genome-wide occupancy associates with distinct epigenetic profiles in mouse fetal liver erythropoiesis.", Nucleic Acids Res., 2013, 41(9), pp. 4938-48. [PubMed].

Papageorgiou DN, Demmers J, Strouboulis J . 2013, "NP-40 reduces contamination by endogenous biotinylated carboxylases during purification of biotin tagged nuclear proteins.", Protein Expr Purif., 2013, 89, pp. 80-83. [PubMed].

2012

Stees, J.S., Varnf, F., Huang, S., Strouboulis J, and Bungert, J. 2012, "Recruitment of Transcription Complexes to Enhancers and the Role of Enhancer Transcription.", Biology, 1(3), 778-793

Brazão TF, Demmers J, van IJcken W, Strouboulis J, , Fornerod M, Romão L, Grosveld FG. 2012, "A new function of ROD1 in nonsense-mediated mRNA decay.", FEBS Lett., 2012, 586(8), pp. 1101-10. [PubMed].

Reynolds N, Latos P, Hynes-Allen A, Loos R, Leaford D, O'Shaughnessy A, Mosaku O, Signolet J, Brennecke P, Kalkan T, Costello I, Humphreys P, Mansfield W, Nakagawa K, Strouboulis J, , Behrens A, Bertone P, Hendrich B. 2012, "NuRD suppresses pluripotency gene expression to promote transcriptional heterogeneity and lineage commitment.", Cell Stem Cell., 2012 May 4; 10(5):583-94. [PubMed].

2011

Leotoing L, Chereau F, Baron S, Hube F, Valencia HJ, Bordereaux D, Demmers JA, Strouboulis J, , Baud V. 2011, "ABIN-2 is an activator of IKK{alpha}-mediated NF-{kappa}B transcriptional activity.", J Biol Chem., 2011, 286(37), pp. 32277-88. [PubMed].

Cui S, Kolodziej K, Obara N, Amaral-Psarris A, Demmers J, Shi L, Engel JD, Grosveld F, Strouboulis J*, , Tanabe O. 2011, "Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Co-repressors that Associate Specifically with the Embryonic {beta}-type Globin Promoters in Differentiated Adult Erythroid Cells.", Mol Cell Biol., Aug;31(16):3298-311 [PubMed]. *Co-corresponding author

Anantharaman A, Lin IJ, Barrow J, Liang SY, Masannat J, Strouboulis J, , Huang S, Bungert J. 2011, "Role of helix-loop-helix proteins during differentiation of erythroid cells.", Mol Cell Biol., 2011 Apr;31(7):1332-43. [PubMed].

2009

Tsiftsoglou AS, Vizirianakis IS, Strouboulis J ., 2009, "Erythropoiesis: Model systems, molecular regulators, and developmental programs", IUBMB Life.; 61(8):800-830., 2009 Jul 20:61(8), 800-830 [Pubmed]

Kolodziej KE, Pourfarzad F, de Boer E, Krpic S, Grosveld F, Strouboulis J. ., 2009, "Optimal use of tandem biotin and V5 tags in ChIP assays.", BMC Mol Biol.;, 2009 Feb 5, 10:6 [Pubmed]

2008

Hamlett I, Draper J, Strouboulis J, Iborra F, Porcher C, Vyas P. 2008 “Characterisation of megakaryocyte GATA1-interacting proteins: the co-repressor ETO2 and GATA1 interact to regulate terminal megakaryocyte maturation.” Blood. Oct 1;112(7) 2738-49. Pubmed

Liang S, Moghimi B, Yang TP, Strouboulis J, Bungert J 2008 “Locus control region mediated regulation of adult beta-globin gene expression”J Cell Biochem.2008 Sep 1;105(1):9-16.Pubmed

2007

Moerland, M., Anghelescu, N., Samyn, H., van Haperen, R., van Gent, T., Strouboulis, J., van Tol, A., Grosveld, F. and de Crom, R. 2007 “Inducible expression of phospholipid transfer protein (PLTP) in transgenic mice: acute effects of PLTP on lipoprotein metabolism.” Transgenic Res. 2007, 16(4), pp. 503-13. Pubmed

2006

Meier, N., Krpic, S., Rodriguez, P., Strouboulis, J., Monti, M., Krijgsveld, J., Gering, M., Patient, R., Hostert, A. and Grosveld,F. 2006 “Novel binding partners of Ldb1 are required for hematopoietic development”. Development. 2006 Dec;133(24):4913-23. PubMed

Rodriguez, P., Braun, H., Kolodziej, K.E., de Boer, E., Campbell, J., Bonte, E., Grosveld, F., Philipsen, S. and Strouboulis, J. 2006 “Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads”. Methods Mol Biol. 2006;338:305-23. PubMed

Horsman, S., Moorhouse, M., de Jager, V., van der Spek, P., Grosveld, F., Strouboulis, J., and Katsantoni, E. 2006 “TFT Mapper: A BLAST search tool for identification of Transcription Factors Target Genes”. BMC Bioinformatics. 2006 Mar 8;7:120. PubMed

Goardon, N., Lambert, J., Rodriguez, P., Herblot, S., Thibault, P., Dumenil, D., Strouboulis, J., Romeo, P-H and Hoang, T. 2006 “ETO2 coordinates cellular proliferation and differentiation during erythropoiesis”. EMBO J. 2006 Jan 25;25(2):357-66. PubMed

2005

Grosveld, F., Rodriguez, P., Meier, N., Krpic, S., Pourfarzad, F., Papadopoulos, P., Kolodziej, K.E., Patrinos, G., Hostert, A. and Strouboulis, J. 2005 “Isolation and characterization of hematopoietic transcription factor complexes by in vivo biotinylation tagging and mass spectrometry”. Ann N Y Acad Sci. 2005;1054:55-67. PubMed

Schuh, A.H., Tipping. A.J., Clark, A.J., Hamlett, I., Guyot, B., Iborra, F.J., Rodriguez, P., Strouboulis, J., Enver, T., Vyas, P. and Porcher C. 2005 “ETO-2 associates with SCL in erythroid cells and megakaryocytes and provides repressor functions in erythropoiesis”. Mol Cell Biol. 2005 Dec;25(23):10235-50. PubMed

Driegen, S., Ferreira, R., van Zon, A., Strouboulis, J., Jaegle, M., Grosveld, F., Philipsen, S. and Meijer, D. 2005 “A generic tool for biotinylation of tagged proteins in transgenic mice”. Transgenic Res. 2005 Aug;14(4):477-82. PubMed

Rodriguez, P., Bonte, E., Krijgsveld, J., Guyot, B., Heck, A., Vyas, P., de Boer, E., Grosveld, F. and Strouboulis, J. 2005 “GATA-1 forms distinct activating and repressive complexes in erythroid cells”. EMBO J. 2005 Jul 6;24(13):2354-66. PubMed

2004

Patrinos, P., de Krom, M., de Boer, E., Langeveld, A., Imam, A., Strouboulis, J., de Laat, W. and Grosveld, F. 2004 “Multiple interactions between regulatory regions are required to stabilize an Active Chromatin Hub”. Genes Dev. 2004 Jun 15;18(12):1495-509. PubMed

Katsantoni, E., de Krom, M., Imam, A. Kong-a-San, J., Grosveld, F., Anagnou, N.P. and Strouboulis, J. 2004 “An embryonic-specific repressor element located 3’ to the Ag-globin gene influences expression of the human b-globin locus in the embryonic stage”. Exp Hematol. 2004 Feb;32(2):224-33. PubMed

2003

Katsantoni, E., Langeveld, A., Wai, A., Drabek, D.,Grosveld, F., Anagnou, N.P.and Strouboulis, J. 2003 “Persistent γ-globin expression in adult transgenic mice is mediated by HPFH-2, HPFH-3, and HPFH-6 breakpoint sequences”. Blood. 2003 Nov 1;102(9):3412-9. PubMed

de Boer, Ε., Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A., Grosveld, F. and Strouboulis, J. 2003 “Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice”. Proc Natl Acad Sci U S A. 2003 Jun 24; 100(13):7480-5. PubMed

2002

de Krom, M.,  van de Corput, M., Grosveld,F. andStrouboulis, J. 2002 “Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited”. Mol Cell. 2002 Jun; 9(6):1319-26. PubMed

1999

Strouboulis, J., Damjanowski, S., Vermaak, D., Meric, F. and Wolffe, A.P. 1999 “Transcriptional repression by XPc1, a new Polycomb homolog in Xenopus laevis embryos, is independent of histone deacetylase” Mol Cell Biol. 1999 Jun;19(6):3958-68. PubMed

1998

Jones, P., Veenstra, G., Wade, P., Vermaak, D., Kass, S., Landsberger, N., Strouboulis, J. and Wolffe, A.P. 1998 “Xenopus MeCP2 exists in a complex with Sin3 and histone deacetylase linking transcriptional repression on methylated DNA to chromatin modification”.Nat Genet. 1998 Jun;19(2):187-91. PubMed

1997

Dillon, N., Trimborn, T., Strouboulis, J., Fraser, P. and Grosveld, F. 1997 “The effect of distance on long range chromatin interactions”. Mol Cell. 1997 Dec;1(1):131-9. PubMed

Grosveld, F., Dillon, N., Fraser, P., Strouboulis, J. and Wijgerde, M. 1997 “The regulation of human β-globin gene expression: the dynamics of transcriptional competition in the human β-globin locus”. NATO Advanced Study Institute on Regulation of Cell Growth, Differentiation and Genetics in Cancer, 1997.

1996

Milot, E., Strouboulis, J., Trimborn, T., Wijgerde, M., de Boer, E., Langeveld, A., Tan-Un, K.,Vergeer, W., Yannoutsos, N., Grosveld, F. and Fraser, P. 1996 “Heterochromatin effects on The frequency and duration of LCR-mediated gene transcription”. Cell. 1996 Oct 4;87(1):105-14. PubMed

Strouboulis, J.and Wolffe, A.P. 1996 “Functional compartmentalization of the nucleus”. J Cell Sci. 1996 Aug;109 ( Pt 8):1991-2000. PubMed

1995

Dillon, N., Strouboulis, J. and Grosveld, F. 1995 “The regulation of human β-globin gene expression: polarity of transcriptional competition in the.human β-globin locus”. Proceedings of the Ninth Conference on Hemoglobin Switching. Ed. G. Stamatoyannopoulos, Intercept Ltd., 1995, pp.23-28.

Ellis, J., Philipsen, S., Strouboulis, J., Pruzina, S., Tewari, R., Gillemans, N., Drabek, D., Harper, A., Grosveld, F. and Fraser, P. 1995 “The regulation of human β-globin gene expression: the roles of 5'HS1-4”. Proceedings of the Ninth Conference on Hemoglobin Switching. Ed. G. Stamatoyannopoulos, Intercept Ltd., 1995, pp.29-38

Grosveld, F., Fraser, P., Philipsen, S., Ellis, J., Wijgerde., M., Antoniou, M., Pruzina, S., Meijer, D., Zafarana, G., Strouboulis, J. and Dillon, N. 1995 “Transcriptional regulation of the β-globin gene cluster”. In: Sickle Cell Disease and Thalassaemias: new trends in therapy. Eds. Y. Beuzard, B. Lubin, J. Rosa. Colloque INSERM/John Libbey Eurotext Ltd., 1995, 234, pp. 19-25.

1994

Müller, A.M., Medvinsky, A., Strouboulis, J., Grosveld, F. and Dzierzak, E. 1994 “Development of hematopoietic stem cell activity in the mouse embryo”. Immunity. 1994 Jul;1(4):291-301. PubMed

1993

Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Hanscombe, O., Hurst, J., Imam, A., Lindenbaum, M., Philipsen, S., Pruzina, S., Strouboulis, J., Raguz-Bolognesi, S. and Talbot, D. 1993 “The Regulation of Human Globin Gene Switching”. Phil. Trans. R. Soc. Lond. B., 1993, 339, pp. 183-191.

Dillon, N., Antoniou, M., Berry, M., de Boer, E., Drabek, D., Ellis, J., Fraser, P., Hanscombe, O., Imam, A., Koken, M-A., Lindenbaum, M., Meijer, D., Philipsen, S., Pruzina, S., Raguz-Bolognesi, S., Strouboulis, J., Talbot, D., Whyatt, D. and Grosveld, F. 1993 “Regulation of the human β-globin expression domain”. In: The Chromosome. Eds. J.S. Heslop-Harrison and R.B. Flavell, Bios Scientific Publishers, 1993, pp. 149-159.

Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Hurst, J., Imam, A., Meijer, D., Philipsen, S., Pruzina, S., Strouboulis, J. and Whyatt, D. 1993 “Regulation of human globin gene switching”. Cold Spring Harbor Symposia on Quantitative Symposia, 1993, Vol. LVIII.

1992

Strouboulis, J., Dillon, N. and Grosveld, F. 1992 “Efficient joining of large DNA fragments for transgenesis”. Nucleic Acids Res. 1992 Nov 25;20(22):6109-10. PubMed

Strouboulis, J., Dillon, N. and Grosveld, F. 1992 “Developmental regulation of the complete 70kb human beta-globin locus in transgenic mice” Genes Dev. 1992 Oct;6(10):1857-64. PubMed

1991

Greaves, D.R., Talbot, D., Philipsen, S., Pruzina, S., de Boer, E., Hanscombe, O., Fraser, P., Antoniou, M., Lindenbaum, M., Dillon, N., Belhumeur, P., Strouboulis, J. and Grosveld, F. 1991 “Studies of the β-globin gene: creation of models for somatic gene therapy”. In: Application of Molecular Genetics to the Diagnosis of Inherited Disease. Royal College of Physicians of London, Ed. K.E. Davies, pp. 77-84.

Dillon, N., Fraser, P., Hanscombe, O., Greaves, D.R., Lindenbaum, M., Whyatt, D., Strouboulis, J., Hurst, J. and Grosveld, F. 1991 “Regulation of the human γ-globin to β-globin switch in transgenic mice”. In: The regulation of Hemoglobin Switching. Proceedings of 7th Hemoglobin Conference. Eds, G. Stamatoyannopoulos and A.W. Nienhuis. Johns Hopkins University Press, Maryland, 1991, pp. 34-44.

Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Greaves, D., Hanscombe, O., Hurst, J., Lindenbaum, M., Mignotte, V., Philipsen, S., Pruzina, S., Strouboulis, J., Talbot, D. and Whyatt, D. 1991 “The human β-globin Locus Control Region” Stohlman Lecture In: Modern Trends in Human Leukemia, 1991, Ed. R. Neth.

Grosveld, F., Antoniou, M., Berry, M., de Boer, E., Dillon, N., Ellis, J., Fraser, P., Greaves, D., Hanscombe, O., Hurst, J., Lindenbaum, M., Mignotte, V., Philipsen, S., Pruzina, S., Strouboulis, J., Talbot, D. and Whyatt, D. 1991 “The regulation of the human β-globin locus. In: Applications of Basic Science to Hematopoiesis and Treatment of Disease”. Ed. Donall Thomas, Raven Press, Ltd., New York, 1991, pp. 21-38.

1990

Samuel, K.P., Ascione, R., Kottarides, S.D., Seth, A.K., Lautenberger, J.A., Zuber, M., Strouboulis, J. and Papas, T.S. 1990 “Expression of animal and human retroviral gene products in Escherichia coli with the lambda pJL6 vector system”. Genet Anal Tech Appl. 1990 Nov;7(7):178-208. PubMed

Grosveld, F., Greaves, D., Philipsen, S., Pruzina, S., de Boer, E., Hanscombe, O., Belhumeur, P., Hurst, J., Fraser, P., Whyatt, D., Antoniou, M., Mignotte, V., Dillon, N., Lindenbaum, M. and Strouboulis, J. 1990 “Τhe Dominant Control Region of the human β-globin domain”. The New York Academy of Sciences, Sixth Cooley's Anemia Symposium Ed. Arthur Bank, 1990, 612, pp. 152-159.

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