imbb mobile logo
 
Χρήστος (Κίτσος) Λούης

Χρήστος (Κίτσος) Λούης

Ερευνητής IMBB (Καθηγητής, Πανεπιστήμιο Κρήτης)
Ηλ. Ταχυδρομείο
Αυτή η διεύθυνση ηλεκτρονικού ταχυδρομείου προστατεύεται από τους αυτοματισμούς αποστολέων ανεπιθύμητων μηνυμάτων. Χρειάζεται να ενεργοποιήσετε τη JavaScript για να μπορέσετε να τη δείτε.
Τηλέφωνο
+30-2810-391119
Fax
+30-2810-391104
Ιστοσελίδα Εργαστηρίου

Η έρευνα στο εργαστήριο μου έχει επικεντρωθεί σε δύο πεδία της βιολογίας των φορέων ασθενειών και της παρασιτολογίας. Αυτά είναι, πρώτον, η μελέτη των μοριακών μηχανισμών που καθορίζουν το πέρασμα του παρασίτου της ελονοσίας μέσα από το μεσέντερο του κουνουπιού και την μετέπειτα ανάπτυξη του Πλασμωδίου στον Ανωφελή φορέα. Δεύτερον, έχουμε εξειδικευθεί στην γονιδιοματική και τη βιοπληροφορική με έμφαση στην κατασκευή ειδικών βάσεων δεδομένων και την ανάπτυξη βιο-οντολογιών. Στην πρώτη περιοχή έχουμε ταυτοποιήσει έναν αριθμό πρωτεϊνών του Ανωφελούς που αλληλεπιδρούν με το Πλασμώδιο επιτρέποντάς του να περάσει τα στάδια του κύκλου ζωής του στο κουνούπι χωρίς να του επιτεθεί το ανοσοποιητικό σύστημα του εντόμου. Επί πλέον, αναλύουμε τους μοριακούς μηχανισμούς την κίνησης του παρασίτου που ευθύνονται για το πέρασμα μέσα από τον φορέα. Στο πεδίο της βιοπληροφορικής η ομάδα μας έχει παίξει έναν κεντρικό, διεθνή ρόλο στην ανάπτυξη γονιδιοματικών και γενετικών βάσεων δεδομένων για τον Ανωφελή και άλλους φορείς ασθενειών, και επίσης είχε την πρωτοπορεία στην κατασκευή οντολογιών στο πεδίο των ασθενειών που μεταδίδονται από αρθρόποδα.

Siden-Kiamos, I., Ganter, M., Kunze, A., Hliscs, M., Steinbüchel, M., Mendoza, J., Sinden, R.E., Louis, C. and Matuschewski, K. (2011) Genetic Strategy for Stage-specific Depletion of Plasmodium Transcripts Supports an Essential Role of Myosin A in Gliding Motility of Malarial Ookinetes. Cell. Microbiol. 13: 1996-2006.

Louis, C. (2011) The dengue fever epidemic in Athens viewed through a daily newspaper in 1927-1931. Emerg. Inf. Dis. 18: 78-82.

Deligianni, E., Morgan, R.N., Bertuccini, L., Kooij, T.W.A., Laforge, A., Poulakakis, N., Schüler, H., Louis, C., Matuschewski, K. and Siden-Kiamos, I. (2011) Critical role for a stage-specific actin in male exflagellation of the malaria parasite. Cell. Microbiol. 13: 1714-30.

Topalis, P., Dialynas, E., Mitraka, E., Deliyanni, E., Siden-Kiamos, I. and Louis C. (2011) A set of ontologies to drive tools for the control of vector-borne diseases. J Biomed. Inform. 44: 42-47.

Lawson, D., Arensburger, P., Atkinson, P., Besansky, N.J., Bruggner, R.V., Butler, R., Campbell, K.S., Christophides, G.K., Dialynas, E., Hammond, M., Hill, C.A., Konopinski, N., Lobo, N.F., MacCallum, R.M., Madey, G., Megy, K., Meyer, J., Redmond, S., Severson, D.W., Stinson, E.O., Topalis, P., Birney, E., Gelbart, W.M., Kafatos, F.C., Louis, C. and Collins, F.H. (2009) VectorBase: a data resource for invertebrate vector genomics. (2009) VectorBase: A home for invertebrate vectors of human pathogens. Nucl. Acids Res. 37: D583-7.

PUBLICATIONS OF THE GROUP (since 2001)

Favia, G., Lanfrancotti, A., Spanos, L., Caruso M., Sidén-Kiamos, I., Coluzzi, M. and Louis, C. (2001). Molecular characterization of ribosomal DNA (rDNA) polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s.. Insect Mol. Biol. 10, 19-23. (PMID: 11240633)

Vlachou*, D., Lycett*, G., Sidén-Kiamos, I., Blass, C., Sinden, R.E. and Louis, C. (2001). Anopheles gambiae laminin interacts with the P25 surface protein of Plasmodium berghei ookinetes. Molec. Biochem. Parasit. 112, 229-237. (PMID: 11223130)

Benos, P.V., Gatt, M.K., Murphy, L., Harris, D., Barrell, B., Ferraz, C., Vidal, S., Brun, C., Demaille, J., Cadieu, E., Dreano, S., Gloux, S., Lelaure, V., Mottier, S., Galibert, F., Borkova, D., Minana, B., Kafatos, F.C., Sidén-Kiamos, I., Bolshakov, S., Papagiannakis, G., Spanos, L., Louis, C., Madueno, E., de Pablos, B., Modolell, J., Peter, A., Schöttler, P., Werner, M., Mourkioti, F., Beinert, N., Dowe, G., Schäfer, U., Jäckle, H., Bucheton, A., Callister, D., Campbell, L., Henderson, N.S., McMillan, P.J., Salles, C., Tait, E., Valenti, P., Pachter, L., Saunders, R.D.C., Glover, D.M. and Ashburner, M. (2001) From First Base: The sequence of the tip of the X chromosome of D. melanogaster. Genome Res., 11, 710-730. (PMID: 11337470)

Mahairaki, V., Lycett, G., Blass, C., and Louis, C. (2001). Beta-integrin of Anopheles gambiae: mRNA cloning and analysis of structure and expression. Insect Mol. Biol. 10: 217-223. (PMID: 11437913)

Louis, C. (2001) Review: a comprehensive set of sequence analysis programs for the VAX. The Biochemist, 23, 64. (PMID: n.a.)

Lycett, G., Blass, C., and Louis, C. (2001). Developmental variation in epidermal growth factor receptor size and localisation in the malaria mosquito, Anopheles gambiae. Insect Mol. Biol., 10, 619-628. (PMID: 11903632)

Bolshakov, V. N. and Louis, C. (2002). Sequencing of complex eukaryotic genomes: man, worm and fruitfly. In: Genome sequencing and comparative analysis (Tsaftaris, A.S., and Polidoros, A.N., eds.) University Studio Press, Thessaloniki. p. 119-131. (PMID: n.a.)

Bolshakov, V., Topalis, P., Blass, C., della Torre, A., Kafatos, F.C. and Louis, C. (2002). A comparison of the genomes of the fruitfly Drosophila melanogaster and the malaria mosquito Anopheles gambiae based on long-range genomic sequences and EST analysis. Genome Res. 12, 57-66. (PMID: 11337470)

Arcà, B., Lombardo, F., Lanfrancotti, A., Spanos, L., Veneri, M., Louis, C. and Coluzzi, M. (2002) A cluster of four D7-related genes are expressed in the salivary glands of the African malaria vector Anopheles gambiae. Insect Mol. Biol.., 11, 47-56. (PMID: 11841502)

Peter, A., Schottler, P., Werner, M., Beinert, N., Dowe, G., Burkert, P., Mourkioti, F., Dentzer, L., He, Y., Deak, P., Benos, P.V., Gatt, M.K., Murphy, L., Harris, D., Barrell, B., Ferraz, C., Vidal, S., Brun, C., Demaille, J., Cadieu, E., Dreano, S., Gloux, S., Lelaure, V., Mottier, S., Galibert, F., Borkova, D, Minana, B., Kafatos, F.C., Bolshakov, S., Siden-Kiamos, I., Papagiannakis, G., Spanos, L., Louis. C, Madueno, E., de Pablos, B., Modolell, J., Bucheton, A., Callister, D., Campbell, L., Henderson, N.S., McMillan, P.J., Salles, C., Tait E., Valenti, P., Saunders, R.D., Billaud, A., Pachter, L., Klapper, R., Janning, W., Glover, D.M., Ashburner, M., Bellen, H.J., Jackle, H., and Schafer, U. (2002) Identification and mapping of vital gene functions of the Drosophila X-chromosome. EMBO Rep. 3, 34-38. (PMID: 11751581)

Holt, R.A., Subramanian, G.M., Halpern, A., Sutton, G.G., Charlab, R., Ribeiro, D.M.C., Wides, R., Salzberg, S.L., Loftus, B., Yandell, M., Majoros, W.H., D.B., Rusch, Lai, Z., Kraft, C.L., Abril, J.F., Anthouard, V., Arensburger, P., Atkinson, P.W., Baden, H., de Berardinis, V., Baldwin, D., Benes, V., Biedler, J., Blass, C., Bolanos, R., Boscus, D., Barnstead, M., Cai, S., Center, A., Chatuverdi, K., Christophides, G.K., Chrystal, M.A., Clamp, M., Cravchik, A., Curwen, V., Dana, A., Delcher, A., Dew, I., Evans, C.A., Flanigan, M., Grundschober-Freimoser, A., Friedli, L., Gu, Z., Guan, P., Guigo, R., Hillenmeyer, M.E., Hladun, S.L., Hogan, J.R., Hong, Y.S., Hoover, J., Jaillon, O., Ke, Z.,1, Kodira, C., Kokoza, E., Koutsos, A., Letunic, I., Levitsky, A., LiangY., Lin, J.J., Lobo, N.F., Lopez, J.R., MalekJ.A., McIntosh, T.C., Meister, S., Miller, J., Mobarry, C., Mongin, E., Murphy, S.D., O’Brochta, D.A., Pfannkoch, C., Qi, R., Regier, M.A., Remington, K., Shao, H., Sharakhova, M., Sitter, C.D., Shetty, J., Smith, T.J., Strong, R., Sun, J., Thomasova, D., Ton, L.Q., Topalis, P., Tu, Z., Unger, M.F., Walenz, B., Wang, A., Wang,J., Wang,M., Wang, X., Woodford, K.J., Wortman,J.R., Wu, M., Yao, A., Zdobnov, E.M., Zhang, H., Zhao, Q., Zhao, S., Zhu, S.C., Zhimulev, I., Coluzzi,M., della Torre,A., Roth, C.W., Louis, C., Kalush, F., Mural, R.J., Myers, E.W., Adams, M.D., Smith, H.O., Broder, S., Gardner, M.J., Fraser, C.M., Birney, E., Bork, P., BreyP.T., Venter, J.C., Weissenbach, J., Kafatos,F.C., Collins, F.H., Stephen L. Hoffman, S.L. (2002) The Genome Sequence of the Malaria Mosquito Anopheles gambiae; Science 298, 129-149. (PMID: 12364791)

Zdobnov, E.M., v. Mering, C., Letunic, I., Torrents, D., Suyama, M., Copley, R.R., Christofides, G.K., Thomasova, D., Holt, R.A., Subramanian, G.M., Mueller, H.M., Dimopoulos, G., Law, J.H., Wells, M.A., Birney, E., Charlab, R., Halpern, A.L., kokoza, E., Kraft, C.L., Zhogwu, L., Lewis, S., Louis, C., Barillas-Mury, C., Nusskern, D., Rubin, G.M., Salzberg, S.L., Sutton, G.G., Topalis, P., Wides, R., Wincker, P., Yandell, M., Collins, F.H., Ribeiro, J., Gelbart, W.M., Kafatos, F.C. and Bork, P. (2002) Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster. Science, 298, 149-159. (PMID: 12364792)

Topalis, P., Koutsos, A. and Louis, C. (2002). An Anopheles Database (AnoBase). Accessible (from October 2002) through WWW at the URL: http://www.anobase.org. (PMID: n.a.)

Parmakelis, A., Spanos, L., Papagiannakis, G., Louis, C. and Mylonas, M. (2003) Mitochondrial DNA phylogeny and morphological diversity in the genus Mastus (Beck, 1837). A study in a recent (Holocene) island group (Koufonissi, SE Crete). Biol. J. Linnean Soc., 78, 383-399. (PMID: n.a., doi:10.1046/j.1095-8312.2003.00152.x )

Louis, C. (2003) Genomics and Medical Entomology: a Sweet and Fruitful Marriage Blessed with Many Children. Rev. Clin. Pharmacol. Pharmacokin. 18, 234-238. (PMID: n.a.).

Dessens, T.J., Sidén-Kiamos, I., Mendoza, J., Macheraki, V., Khater, E., Vlachou, D., Xu, X.J., Kafatos, F.C., Louis, C., Dimopoulos, G. and Sinden, R.E. (2003). SOAP, a novel malaria ookinete protein involved in mosquito midgut invasion and oocyst development. Mol. Microbiol., 449, 319-329. (PMID: 12828632)

Louis, C. (2003). Medical entomology and genomics [in Greek]. BIO, 7, 34-38. (PMID: n.a.)

Louis, C. (2003). Biology and the Orthodox Academy of Crete. [in Greek] Dialogoi Katallagis, 71, 714-716. (PMID: n.a.)

Louis, C. (2003). Gene Bank Data for Hematophagous Arthropods. In: The Biology of Disease Vectors, Second Edition (Marquardt, W.C., Black, W.C., Freier, J., Hagedorn, H., Hemingway, J., Higgs, S., James, A.A. and Kondratieff, B., eds.) Academic Press. pp.693-697, ISBN: 0-12-473276-3 (PMID: n.a.)

Milgram, A. J., Birney, E., Jasny, B. R., Collins, F., Louis, C., and Roos, D. R. (2003). The Mosquito Genome. CD published by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) and the American Association for the Advancement of Science (Science). (PMID: n.a.)

James, A.A. and Louis, C., Rapporteurs (2003) Insect Vectors and Human Health. Report of the Scientific Working Group meeting, 12-16 August 2002, Geneva, Switzerland. WHO/TDR Publications, pp.1-73. (PMID: n.a., download from TDR)

Mongin, M., Louis, C., Holt, R. A., Birney, E. and Collins, F. H. (2004). The Anopheles gambiae genome: an update on sequence, data, annotation and access. Trends Parasitol., 20, 49-52. (PMID: 14747013)

Louis, C. (2004). Preface. Insect Biochem. Molec. Biol., 34, 597. (PMID: n.a., doi:10.1016/j.ibmb.2004.03.011)

Siden-Kiamos, I. and Louis, C. (2004). Interactions between malaria parasites and their mosquito hosts in the midgut Insect Biochem. Molec. Biol., 34, 679-685. (PMID: 15242709).

Vontas, J., McCarroll, L., Karunaratne, S.H.P.P., Louis, C., Hurd, H. and Hemingway, J. (2004) Does environmental stress affect insect-vectored parasite transmission? Physiol. Entomol. 29, 210-213. (PMID: n.a., doi:10.1111/j.0307-6962.2004.00410.x).

Ribeiro, J.M.C., Topalis, P. and Louis, C. (2004) AnoXcel: A database of Anopheles gambiae proteins oriented to the bench scientist. Insect Mol. Biol. 13, 449-457 (PMID: 15373803).

Vontas, J., Sidén-Kiamos, I., Papagiannakis, G., Karras, M., Waters, A.P. and Louis, C. (2005) Gene expression in Plasmodium berghei during invasion in an Aedes- parasite co-culture in vitro. Molec. Biochem. Parasit. 139, 1-13 (PMID: 15610814).

Mahairaki, V., Voyatzi, T., Siden-Kiamos, I. and Louis, C. (2005). The Anopheles gambiae gamma1 laminin directly binds the Plasmodium berghei circumsporozoite- and TRAP-related protein (CTRP). Molec. Biochem. Parasit. 140, 119-121. (PMID: 15694493).


David, J.P., Strode, C., Vontas, J., Nikou, D., Vaughan, A., Pignatelli, P.M., Louis, C., Hemingway, J. and Ranson, H. (2005) The Anopheles gambiae Detoxification Chip: a new highly specific microarray to study metabolic based insecticide resistance in malaria vectors. Proc. Natl. Acad. Sci. USA 102, 4080-4084. (PMID: 15753317)

Lombardo, F., Nolan, T., Lycett, G., Lanfrancotti, A., Stich, N., Catteruccia, F., Louis, C., Coluzzi, M. and Arca, B. (2005) An Anopheles gambiae salivary gland promoter analysis in Drosophila melanogaster and Anopheles stephensi. Insect Mol. Biol. 14, 207-216. (PMID: 15796754).

Kotsyfakis, M., Ehret-Sabatier, L., Siden-Kiamos, I., Mendoza, J., Sinden, R.E. and Louis, C. (2005) Plasmodium berghei ookinetes bind to Anopheles gambiae and Drosophila melanogaster annexins. Molec. Microbiol.; 57, 171-179. (PMID: 15948958).

Parmakelis, A., Pfenninger, M., Spanos, L., Papagiannakis, G., Louis, C., and Mylonas, M. (2005) Inference of a radiation in Mastus (Gastropoda, Pulmonata, Enidae) on the island of Crete. Evolution 59, 991-1005. (PMID: 16136799).

Arrighi, R.B.G., Lycett, G., Mahairaki, V., Sidén-Kiamos, I. and Louis, C. (2005) Laminin and the malaria parasite’s journey through the mosquito midgut. J. Exp. Biol., 208, 2497-2502. (PMID: 15961736).

Louis, C. (2005) O Mosquito transgenico [in Portuguese]. Ciencia Hoje 36, 6-8.(PMID: n.a.).

Knols, B.G.J. and Louis, C., eds. (2005) Bridging laboratory and field research for genetic control of disease vectors. (pp. i-x and 1-225). Wageningen UR Frontis Series, Vol. 11, Springer, Heidelberg, Germany, ISBN: 1-4020-3799-6. (PMID: n.a.).

Louis, C. and Knols, B.G.J. (2005) Report of the Working group meeting. In Bridging laboratory and field research for genetic control of disease vectors. (Knols, B. and Louis, C., eds.) Wageningen UR Frontis Series, Vol. 11, Springer, Heidelberg, Germany, ISBN: 1-4020-3799-6. (PMID: n.a.).

Louis, C. (2005) Genomics and expected benefits for vector entomology. In Bridging laboratory and field research for genetic control of disease vectors. (Knols, B. and Louis, C., eds.) Wageningen UR Frontis Series, Vol. 11, Springer, Heidelberg, Germany, ISBN: 1-4020-3799-6. (PMID: n.a.).

Louis, C. (2005) Transition from the laboratory to field and effective interplay. In Bridging laboratory and field research for genetic control of disease vectors. (Knols, B. and Louis, C., eds.) Wageningen UR Frontis Series, Vol. 11, Springer, Heidelberg, Germany, ISBN: 1-4020-3799-6. (PMID: n.a.).

Mahairaki, V., Lycett, G., Sidén-Kiamos, I., Sinden, R.E. and Louis, C. (2005). Close association of invading Plasmodium berghei and beta integrin in the Anopheles gambiae midgut. Arch. Insect Biochem. Physiol. 20, 13-19. (PMID: 16116619)

Vontas, J., Blass, C., Koutsos, A.C., David, J.P., Kafatos, F.C., Louis, C., Hemingway, J., Christophides, G.K., Ranson, H. (2005) Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure. Insect Mol. Biol. 14, 509-521. (PMID: 16164607)

Kotsyfakis, M., Vontas, J., Siden-Kiamos and Louis, C. (2005) The annexin gene family in the malaria mosquito Anopheles gambiae. Insect Mol. Biol. 14, 555-562. (PMID: 16164611)

Topalis, P., Koutsos, A.C., Dialynas, M., Kiamos, C., Hemingway, J. and Louis, C. (2005) AnoBase: A genetic and biological database of anophelines Insect Mol. Biol. 14, 591-597. (PMID: XXX)