imbb mobile logo
 
Αχιλλέας Γραβάνης

Αχιλλέας Γραβάνης

Ερευνητής IMBB (Καθηγητής, Πανεπιστήμιο Κρήτης)
Ηλ. Ταχυδρομείο
Αυτή η διεύθυνση ηλεκτρονικού ταχυδρομείου προστατεύεται από τους αυτοματισμούς αποστολέων ανεπιθύμητων μηνυμάτων. Χρειάζεται να ενεργοποιήσετε τη JavaScript για να μπορέσετε να τη δείτε.
Τηλέφωνο
+30-2810-394521
Fax
+30-2810-391104
Ιστοσελίδα Εργαστηρίου

Το ενδιαφέρον μας εστιάζεται στην ανάπτυξη μικρομοριακών συνδετών-αγωνιστών των υποδοχέων των Νευροτροφικών με νευροπροστατευτικές και νευροαναγεννητικές δράσεις και πιθανές εφαρμογές στην θεραπεία των νευροεκφυλιστικών νόσων και της εγκεφαλικής γήρανσης. Επίσης,  αναπτύσσουμε τρισδιάστατα ικριώματα στα οποία καλλιεργούμε νευρικά βλαστικά κύτταρα και ολιγοδενδροκύτταρα με στόχο την ανάπτυξη ι) νευρο-εμφυτευμάτων για την θεραπεία του τραύματος του νωτιαίου μυελού καθώς και ιι) νευρο-αισθητήρων για την ανίχνευση ουσιών με νευροαναγεννητική δράση και πιθανές φαρμακολογικές εφαρμογές στην ανάπλαση του πάσχοντος ή γηράσκοντος νευρικού ιστού.

Ανάπτυξη μικρομοριακών συνδετών-αγωνιστών των υποδοχέων των Νευροτροφικών με νευροπροστατευτικές και νευροαναγεννητικές δράσεις.

Η ανάπτυξη, η διαφοροποίηση αλλά και η λειτουργία του εγκεφάλου υπόκειται στο έλεγχο των Νευροτροφινών, όπως ο Νευρωνικός Αυξητικός Παράγων (Nerve Growth Factor, NGF). Η ελλειμματική παραγωγή ή δράση των Νευροτροφινών έχει συσχετιστεί με τα φαινόμενα εκφύλισης και γήρανσης του εγκεφάλου, πειραματικά δε και κλινικά δεδομένα συνηγορούν στην ευεργετική τους δράση στην θεραπεία των νευροεκφυλιστικών νόσων. Εν τούτοις, η πεπτιδική τους φύση περιορίζει την εφαρμογή τους στην κλινική πρακτική. Αναπτύσσουμε μικρομοριακές ενώσεις με χημική συγγένεια για τους κυτταρικούς υποδοχείς των Νευροτροφινών και ισχυρές νευροπροστατευτικές και νευροτροφικές δράσεις (Μικρονευροτροφίνες), οι οποίες διέρχονται τον αιματεγκεφαλικό φραγμό. Η δραστικότητα των συνθετικών Μικρονευροτροφινών in vivo ελέγχεται σε πειραματόζωα, πειραματικά μοντέλα των νευροεκφυλιστικών νόσων του ανθρώπου (Σκλήρυνση κατά Πλάκας, Νόσος Αλτζχαιμερ, κλπ).

Τρισδιάστατα ικριώματα καλλιέργειας  νευρικών βλαστικών κυττάρων: ανάπτυξη νευρο-εμφυτευμάτων και νευρο-αισθητήρων.

Τα τελευταία χρόνια έχει προταθεί η εφαρμογή των νευρικών βλαστικών κυττάρων στην θεραπευτική των νευροεκφυλιστικών νόσων και του εγκεφαλικού τραύματος. Αναπτύσσουμε τρισδιάστατα μικρο-νανο-ικριώματα καλλιέργειας νευρικών βλαστικών κυττάρων και ολιγοδενδροκυττάρων με στόχο την νευροβιολογική και νευροχημική διερεύνηση των φαινομένων του πολλαπλασιασμού, διαφοροποίησης, συναπτογένεσης και νευριτογένεσης των νευρικών βλαστικών κυττάρων σε συνθήκες τρισδιάστατης καλλιέργειας που προσομοιάζει περισσότερο στον ζώντα εγκέφαλο. Η τεχνολογία των τρισδιάστατων ικριωμάτων καλλιέργειας  νευρικών βλαστικών κυττάρων  στοχεύει στην ανάπτυξη νευρο-εμφυτευμάτων και νευρο-αισθητήρων για την ανίχνευση νέων νευροαναγεννητικών ουσιών με πιθανές εφαρμογές στην ανάπλαση του υπό εκφύλιση νευρικού ιστού.

Pediaditakis I, Efstathopoulos P, Prousis KC, Zervou M, Arévalo JC, Alexaki VI, Nikoletopoulou V, Karagianni E, Potamitis C, Tavernarakis N, Chavakis T, Margioris AN, Venihaki M, Calogeropoulou T, Charalampopoulos I, Gravanis A. (2016) Selective and differential interactions of BNN27, a novel C17-spiroepoxy steroid  derivative, with TrkA receptors, regulating neuronal survival and differentiation. Neuropharmacology 11:266-282.

Simitzi C, Efstathopoulos P, Kourgiantaki A, Ranella A, Charalampopoulos I, Fotakis C, Athanassakis I, Stratakis E, Gravanis A. (2015) Laser fabricated discontinuous anisotropic microconical substrates as a new model scaffold to control the directionality of neuronal network outgrowth. Biomaterials 67:115-128.

Lazaridis I, Charalampopoulos I, Alexaki VI, Avlonitis N, Pediaditakis I, Efstathopoulos P, Calogeropoulou T, Castanas E, Gravanis A. (2011) Neurosteroids interacts with nerve growth factor (NGF) receptors, preventing neuronal apoptosis. PLoS Biol 9:e1001051.

Calogeropoulou T, Avlonitis N,  Minas V, Pantzou A, Alexi X, Charalampopoulos I, Zervou M, Vergou V, Lazaridis I, Alexis MN, Gravanis A. (2009) Novel dehydroepiandrosterone derivatives with anti-apoptotic, neuroprotective activity.  J Med Chem 52:6569-6587.
    
Charalampopoulos I, Tsatsanis C, Dermitzaki E, Alexaki I, Castanas E, Margioris AN, Gravanis A. (2004) Dehydroepiandrosterone and allopregnanolone protect sympathoadrenal cells against apoptosis, via Bcl-2 antiapoptotic proteins. PNAS 101:8209-8214.

2017

Botsakis K, Mourtzi T, Panagiotakopoulou V, Vreka M, Stathopoulos GT, Pediaditakis I, Charalampopoulos I, Gravanis A, Delis F, Antoniou K, Zisimopoulos D, Georgiou CD, Panagopoulos NT, Matsokis N, Angelatou F. BNN-20, a synthetic microneurotrophin, strongly protects dopaminergic neurons in the "weaver" mouse, a genetic model of dopamine-denervation, acting through the TrkB neurotrophin receptor. Neuropharmacology. 2017 Apr 28;121:140-157

Pitsikas N, Gravanis A. The novel dehydroepiandrosterone (DHEA) derivative BNN27 counteracts delay-dependent and scopolamine-induced recognition memory deficits in rats. Neurobiol Learn Mem. 2017 Apr;140:145-153.

Gravanis A, Pediaditakis I, Charalampopoulos I. Synthetic microneurotrophins in therapeutics of neurodegeneration. Oncotarget. 2017 Feb 7;8(6):9005-9006
  
2016

Pediaditakis I, Kourgiantaki A, Prousis KC, Potamitis C, Xanthopoulos KP, Zervou M, Calogeropoulou T, Charalampopoulos I, Gravanis A.(2016) BNN27, a 17-Spiroepoxy Steroid Derivative, Interacts With and Activates p75 Neurotrophin Receptor, Rescuing Cerebellar Granule Neurons from Apoptosis. Front Pharmacol. 2016 Dec 26;7:512. doi: 10.3389/fphar.2016.00512

Pediaditakis I, Efstathopoulos P, Prousis KC, Zervou M, Arévalo JC, Alexaki VI, Nikoletopoulou V, Karagianni E, Potamitis C, Tavernarakis N, Chavakis T, Margioris AN, Venihaki M, Calogeropoulou T, Charalampopoulos I, Gravanis A. Selective and differential interactions of BNN27, a novel C17-spiroepoxy steroid derivative, with TrkA receptors, regulating neuronal survival and differentiation. Neuropharmacology. 2016 Dec;111:266-282.

Glajch KE, Ferraiuolo L, Mueller KA, Stopford MJ, Prabhkar V, Gravanis A, Shaw PJ, Sadri-Vakili G. MicroNeurotrophins Improve Survival in Motor Neuron-Astrocyte Co-Cultures but Do Not Improve Disease Phenotypes in a Mutant SOD1 Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One. 2016 Oct 7;11(10):e0164103. doi: 10.1371/journal.pone.0164103  

Vazgiouraki E, Papadakis VM, Efstathopoulos P, Lazaridis I, Charalampopoulos I, Fotakis C, Gravanis A. Application of multispectral imaging detects areas with neuronal myelin loss, without tissue labelling. Microscopy (Oxf). 2016 Apr;65(2):109-18

2015

Simitzi C, Efstathopoulos P, Kourgiantaki A, Ranella A, Charalampopoulos I, Fotakis C, Athanassakis I, Stratakis E, Gravanis A. (2015) Laser fabricated discontinuous anisotropic microconical substrates as a new model scaffold to control the directionality of neuronal network outgrowth. Biomaterials. 67:115-128

Efstathopoulos P, Kourgiantaki A, Karali K, Sidiropoulou K, Margioris AN, Gravanis A, Charalampopoulos I. Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory. Transl Psychiatry. 2015 Nov 24;5:e685. doi: 10.1038/tp.2015.179

Pediaditakis I, Iliopoulos I, Theologidis I, Delivanoglou N, Margioris AN, Charalampopoulos I, Gravanis A. Dehydroepiandrosterone: An Ancestral Ligand of Neurotrophin Receptors, Endocrinology. 156(1):16-23

2013
   
Anagnostopoulou V, Pediaditakis I, Alkahtani S, Alarifi SA, Schmidt EM, Lang F, Gravanis A, Charalampopoulos I, Stournaras C. Differential effects of dehydroepiandrosterone and testosterone in prostate and colon cancer cell apoptosis: the role of nerve growth factor (NGF) receptors. Endocrinology 154(7), 2446-56.
   
2012
   
Charalampopoulos I., Vicario A., Pediaditakis I., Gravanis A., Simi A., Ibanez CF. Genetic Dissection of Neurotrophin Signaling through the p75 Neurotrophin Receptor. Cell Reports 2, 1563-1570
   
Gravanis A, Calogeropoulou T, Panoutsakopoulou V, Thermos K, Neophytou C, Charalampopoulos I.(2012) Neurosteroids and Microneurotrophins Signal Through NGF Receptors to Induce Prosurvival Signaling in Neuronal Cells. Science Signaling. 16;5(246):pt8.
   
Notas G, Alexaki VI, Kampa M, Pelekanou V, Charalampopoulos I, Sabour-Alaoui S, Pediaditakis I, Dessirier V, Gravanis A, Stathopoulos EN, Tsapis A, Castanas E. (2012) APRIL Binding to BCMA Activates a JNK2-FOXO3-GADD45 Pathway and Induces a G2/M Cell Growth Arrest in Liver Cells. J Immunol. 2012 Oct 15. [Epub ahead of print]
   
Kokona D, Charalampopoulos I, Pediaditakis I, Gravanis A, Thermos K. (2012) The neurosteroid dehydroepiandrosterone (DHEA) protects the retina from AMPA-induced excitotoxicity: NGF TrkA receptor involvement. Neuropharmacology 62:2106-2117.
   
Chrousos GP, Kalantaridou SN, Margioris AN, Gravanis A. (2012) The 'self-plagiarism' oxymoron: can one steal from oneself? Eur J Clin Invest. 42:231-232
   
Ioannou M, Alissafi T, Lazaridis I, Deraos G, Matsoukas J, Gravanis A, Mastorodemos V, Plaitakis A, Sharpe A, Boumpas D, Verginis P. (2012) Crucial role of granulocytic myeloid-derived suppressor cells in the regulation of central nervous system autoimmune disease. J Immunol. 188:1136-1146
   
2011

Lazaridis I, Charalampopoulos I, Alexaki VI, Avlonitis N, Pediaditakis I, Efstathopoulos P, Calogeropoulou T, Castanas E, Gravanis A. (2011) Neurosteroids interacts with nerve growth factor (NGF) receptors, preventing neuronal apoptosis. PLoS Biol 9(4):e1001051.
   
Rassouli O, Liapakis G, Lazaridis I, Sakellaris G, Gkountelias K, Gravanis A, Margioris AN, Karalis KP, Venihaki M. (2011). A novel role of peripheral corticotropin-releasing hormone (CRH) on dermal fibroblasts. PLoS One 6(7):e21654.
   
Dermitzaki E, Tsatsanis C, Gravanis A, Margioris AN. (2011) The calcineurin - nuclear factor of activated T cells signaling pathway mediates the effect of corticotropin releasing factor and urocortins on catecholamine synthesis. J Cell Physiol. 2011 Jun 23. doi: 10.1002/jcp.22914
   
Fragkouli A, Tzinia AK, Charalampopoulos I, Gravanis A, Tsilibary EC. (2011) Matrix Metalloproteinase-9 Participates in NGF-Induced α-Secretase Cleavage of Amyloid-β Protein Precursor in PC12 Cells. J Alzheimer Dis. 24:705-719.
   
2009
   
Toubouraki D, Christopoulos T, Ioannou P, Gravanis A. (2009) Visual Genotyping of Single Nucleotide Polymorphisms of Drug Metabolizing Enzymes by Tetra-Primer PCR Coupled with a Dry-Reagent Disposable Biosensor. Pharmacogenomics, 3:495-504
   
Gkountelias K, Tselios T, Venihaki M, Deraos M, Lazaridis I, Rassouli O, Gravanis A, Liapakis G. (2009). Alanine scanning mutagenesis of the second extracellular loop of type 1 corticotropin releasing factor receptor revealed residues critical for peptide binding. Mol Pharmacol 75:793-800
   
Calogeropoulou T, Avlonitis N,  Minas V, Pantzou A, Alexi X, Charalampopoulos I, Zervou M, Vergou V, Lazaridis I, Alexis MN, Gravanis A. (2009). Novel dehydroepiandrosterone derivatives with anti-apoptotic, neuroprotective activity.  J Med Chem, 52:6569-6587
   
2008
   
Charalampopoulos I, Margioris A, Gravanis A. (2008). Neurosteroid dehydroepiandrosterone exerts anti-apoptotic effects by membrane-mediated, integrated genomic and non-genomic pro-survival signaling pathways. J Neurochem, 107:1457-1469
   
Toubouraki D, Christopoulos T, Ioannou P, Gravanis A. (2008) Dry-reagent disposable biosensor for visual genotyping of single nucleotide polymorphisms by oligonucleotide ligation: Application to pharmacogenetic analysis. Hum Mut, 29:1071-1078
   
Papadopoulou N, Charalampopoulos I, Alevizopoulos K, Gravanis A, Stournaras C. (2008) Rho/ROCK/actin signaling regulates membrane androgen receptor induced apoptosis in prostate cancer cells. Exp Cell Res. 314:3162-3174
   
Papadopoulou N, Charalampopoulos I, Anagnostopoulou V, Konstantinidis G, Föller M, Gravanis A, Alevizopoulos K, Lang F, Stournaras C. (2008) Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells. Mol Cancer, 3;7:88
   
Charalampopoulos I, Remboutsika E. Margioris A, Gravanis A. (2008) Neurosteroids as endogenous modulators of neurogenesis and neuronal survival. Trends Endocrinol Metab 19:300-307
   
2007
   
Tsatsanis C, Dermitzaki E, Venihaki M, Chatzaki E, Minas V, Gravanis A, Margioris AN. (2007) The corticotropin-releasing factor (CRF) family of peptides as local modulators of adrenal function. Cell Mol Life Sci. 64:1638-1655
   
Litos I, Emmanouilidou E, Glynou K, Laios E, Ioannou P, Christopoulos T, Kampa M, Castanas E, Gravanis A.  (2007). Rapid Genotyping of CYP2D6, CYP2C19 and TPMT Polymorphisms by Primer Extension Reaction in a Dipstick Format. Anal. Bioanal. Chem. 389:1849-1857
   
Tsatsanis C, Androulidaski A, Dermitzaki E, Gravanis A, Margioris A. (2007) Corticotropin Releasing Factor receptor 1 (CRF1) and CRF2 Agonists exert an anti-inflammatory effect during the early phase of inflammation suppressing LPS-induced TNF-α release from macrophages via induction of COX-2 and PGE2. J Cell Physiol, 210:774-783
   
Dermitzaki E, Tsatsanis C, Minas V, Chatzaki E, Venihaki M, Androulidaki A, Lambropoulou M, Spiess S, Gravanis A, Margioris A. (2007). Corticotropin Releasing Factor (CRF) and the Urocortins differentially regulate catecholamine secretion from human and rat adrenals in a CRF receptor type specific manner. Endocrinology, 148:1524-1538
   
2006
   
Charalampopoulos I, Alexaki IV, Lasaridis I, Dermitzaki E, Avlonitis N,  Tsatsanis C, Calogeropoulou T, Margioris A, Castanas E, Gravanis A.  (2006). G-protein-associated membrane binding sites mediate the neuroprotective effect of Dehydroepiandrosterone. FASEB J, 20:577-579
   
Tsatsanis C, Androulidaki A, Charalampopoulos I, Gravanis A, Margioris A. (2006). Corticotropin-Releasing Factor (CRF) and the Urocortins Induce the Expression of Toll Like Receptor-4 (TLR4) in Macrophages via Activation of the Transcription Factor PU.1. J Immunol, 176:1869-1877
   
Kampa M, Kogia C, Theodoropoulos PA, Anezinis P, Charalampopoulos I, Papakonstanti EA, Stathopoulos EN, Hatzoglou A, Stournaras C, Gravanis A, Castanas E. (2006). Activation of membrane androgen receptors potentiates the antiproliferative effects of paclitaxel on human prostate cancer cells. Mol Cancer Ther. 5:1342-1351.
   
Alexaki VI, Charalampopoulos I, Kampa M, Nifli AP, Hatzoglou A, Gravanis A, Castanas E. (2006). Activation of membrane estrogen receptors induce pro-survival kinases. J Steroid Biochem Mol Biol. 98:97-110.
   
Alexaki VI, Dermitzaki E, Charalampopoulos I, Kampa M, Nifli AP, Gravanis A, Margioris AN, Castanas E. (2006). Neuronal differentiation of PC12 cells abolishes the expression of membrane androgen receptors. Exp Cell Res. 312:2745-2756
   
Charalampopoulos I, Androulidaki A, Minas V, Chatzaki E, Tsatsanis C, Notas G, Xidakis C, Kollios G, Kouroumalis E, Margioris A. Gravanis A. (2006) Neuropeptide Urocortin and its receptors: are expressed in rat Kupffer cells. Neuroendocrinology, 84:49-57
   
Charalampopoulos I, Alexaki VI, Tsatsanis C, Minas V, Dermitzaki E, Lasaridis I, Vardouli L,  Stournaras C, Margioris A, Castanas E, Gravanis A. (2006). Neurosteroids, as endogenous inhibitors of neuronal cell apoptosis in ageing. Ann NY Acad Sci 1088:139-52
   
Tsatsanis C, Zacharioudaki V, Androulidaki A, Dermitzaki E, Charalampopoulos I, Minas V, Gravanis A, Margioris A. (2006). Peripheral factors in the metabolic syndrome: the pivotal role of adiponectin. Ann NY Acad Sci 1083:185-95.
   
2005
   
Gravanis A, Margioris AN. (2005).The corticotropin-releasing factor (CRF) family of neuropeptides in inflammation: potential therapeutic applications. Curr Med Chem. 12:1503-1512.
   
Dermitzaki E, Tsatsanis C, Charalampopoulos I, Gravanis A, Margioris AN. (2005). Corticotropin-releasing hormone activates protein kinase C in an isoenzyme-specific manner. Biochem Biophys Res Commun 327:828-836
   
Hatzoglou A, Kampa M, Kogia C, Charalampopoulos I, Theodoropoulos P, Anezinis P, Dambaki C, Papakonstanti E, Stathopoulos E, Stournaras C, Gravanis A, Castanas E. (2005). Membrane androgen receptor activation induces apoptotic regression of human prostate cancer cells in vitro and in vivo. J Clin Endocrinol Metab, 90:893-903
   
Tsatsanis C, Androulidaki A, Dermitzaki E, Charalampopoulos I, Spiess J, Gravanis A, Margioris AN. (2005) Urocortin 1 and Urocortin 2 induce macrophage apoptosis via CRFR2. FEBS Lett. 579:4259-4264.
   
Charalampopoulos I, Dermitzaki E, Vardouli L, Tsatsanis C, Stournaras C, Margioris A, Gravanis A.  (2005) Dehydroepiandrosterone Sulfate and Allopregnanolone Directly Stimulate Catecholamine Production via Induction of Tyrosine Hydroxylase and Secretion by Affecting Actin Polymerization. Endocrinology. 146:3309-3318.
   
Kampa M, Nifli AP, Charalampopoulos I, Alexaki VI, Theodoropoulos PA, Stathopoulos EN, Gravanis A, Castanas E. (2005). Opposing effects of estradiol- and testosterone-membrane binding sites on T47D breast cancer cell apoptosis. Exp Cell Res. 307:41-51.
   
Tsatsanis C, Zacharioudaki V, Androulidaki A, Dermitzaki E, Charalampopoulos I, Minas V, Gravanis A, Margioris AN. (2005) Adiponectin induces TNF-alpha and IL-6 in macrophages and promotes tolerance to itself and other pro-inflammatory stimuli. Biochem Biophys Res Commun. 335:1254-1263.
   
2004
   
Kampa M, Nistikaki A, Hatzoglou A, Bakogeorgou E, Kouimtzoglou E, Blekas G, Boskou D,  Gravanis A, Castanas E. (2004). Growth inhibitory effects of olive oil phenolic acids on T47D human breast cancer cells. Breast Cancer Res, 6:63-74
   
Charalampopoulos I, Tsatsanis C, Dermitzaki E, Alexaki I, Castanas E, Margioris AN, Gravanis A. (2004). Dehydroepiandrosterone and allopregnanolone protect sympathoadrenal cells against apoptosis, via Bcl-2 antiapoptotic proteins. Proc Natl Acad Sci USA, 101:8209-8214
   
Alexaki V.I, Charalampopoulos I, Kampa M, Vassalou E, Theodoropoulos P, N. Stathopoulos EN, Hatzoglou A, Gravanis A, Castanas E. (2004). Estrogen exert neuroprotective effects via membrane estrogen receptors and rapid Akt/NOS activation. FASEB J. 13:1594-1596
   
2003

Chatzaki E, Charalampopoulos I, Leontidis C, Mouzas I, Tsardi M, Tsatsanis C, Margioris A, Gravanis A. (2003). Urocortin (Ucn) expression in human gastric mucosa: relationship to inflammatory activity. J Clin Endocrinol Metab 88:478-483

Chatzaki E, Kouimtzoglou E, Margioris A, Gravanis A. (2003). Transforming Growth Factor-β1 (TGFβ1) exerts an autocrine regulatory effect of human endometrial stromal cell apoptosis, involving the FasL and Bcl-2 pathways. Mol Hum Reprod 9:91-95
   
2002
   
Chatzaki E, Margioris A, Gravanis A. (2002). Expression and regulation of Corticotropin-Releasing Hormone Binding Protein (CRH-BP) in rat adrenal cells. J Neurochem, 80:81-90
   
Dermitzaki E, Tsatsanis C, Gravanis A, Margioris A. (2002). Corticotropin-releasing hormone induces apoptosis in PC12 cells through Fas Ligand and p38MAPK kinase. J Biol Chem, 277:12280-12287.
   
Agelaki S, Tsatsanis C, Gravanis A, Margioris, AN. (2002). Corticotropin-releasing hormone augments proinflammatory cytokine production from macrophages in vitro and in lipopolysaccharide-induced endotoxin shock in. Infect Immun 70:6068-6074.
   
Chatzaki E, Charalampopoulos I, Leontidis C, Mouzas I, Tsardi M, Tsatsanis C, Margioris A, Gravanis A. (2003). Urocortin (Ucn) expression in human gastric mucosa: relationship to inflammatory activity. J Clin Endocrinol Metab 88:478-483
   
2001
   
Gravanis A, Makrigiannakis A, Zoumakis E, Margioris A. (2001) Endometrial and Myometrial Corticotropin Releasing Hormone (CRH) : its regulation and possible roles. Peptides, 22:785-793
   
Zoumakis E, Chatzaki E, Charalampopoulos G, Margioris A, Angelakis E, Koumantakis E, Gravanis A. (2001) Cycle and age changes of  human endometrial and ovarian corticotropin-releasing hormone. Gynecol Endocrinol, 15:98-102
   
Dermitzaki E, Gravanis A, Venihaki M, Stournaras C, Margioris A. (2001). Opioids suppress basal and nicotine-induced catecholamine secretion via a stabilizing effect on actin filaments. Endocrinology, 142:2022-2031
   
Krasagakis K, Almond-Roesler B, Geilen C, Fimmel S, Krengel S, Chatzaki E, Gravanis A, Orfanos CE. (2001). Growth and characterization of a cell line from a human primary neuroendocrine carcinoma of the skin (Merkel Cell Carcinoma) in culture and as xenograft. J Cell Physiol 187:386-391
   
Chatzaki E, Makrigiannakis A, Margioris A,  Kouimtzoglou E, Gravanis A. (2001). The Fas/FasL apoptotic pathway is involved in kappa-opioid induced apoptosis in human endometrial stromal cells: Mol Hum Reprod, 7:867-874
   
Makrigiannakis A, Zoumakis E, Kalantaridou S, Coutifaris, Margioris A, C, K. Rice, #Gravanis A/#Chrousos G  (2001). Corticotropin-releasing Hormone (CRH) Promotes Blastocyst Implantation and Early Maternal Tolerance. Nature Immunol, 2:1018-1024 (# both authors contributed equally to this work)
   
2000
   
Dermitzaki I, E. Chatzaki E, Gravanis A, Margioris A. (2000). Opioids transiently prevent activation of apoptotic mechanisms following short periods of serum deprivation. J Neurochem, 74:960-969
   
Zoumakis E, Margioris A, Stournaras C, Dermitzaki I, Angelakis E, Makrigiannakis A, Koumantakis E, Gravanis A. (2000). Corticotropin-releasing hormone (CRH) interacts with inflammatory prostaglandins and interleukins and affects decidualization of human endometrial stroma. Mol Human Reprod, 6:101-108

Papakostanti E, Emmanouel D, Gravanis A, Stournaras C. (2000). PCL-γ1 signaling and villin activation are involved in actin cytoskeleton reorganization induced by the Na/Pi co-transport upregulation. Mol Med, 6:303-318
   
Chatzaki E, Margioris A, Makrigiannakis A, Castanas E, Gravanis A. (2000). Kappa Opioids and Transforming Growth Factor beta 1 interact in human endometrial cells. Mol Human Reprod, 6:602-609
   
1999
   
Gravanis A, Stournaras C, Margioris A. (1999). Paracrinology of endometrial neuropeptides: CRH and opioids Sem Reprod Endocrinol, 17:29-38
   
Kampa M, Margioris AN, Hatzoglou A, Dermitzaki E, Gravanis A, Henry J, Oliver C, Castanas E. (1999). Kappa1 are the dominant binding sites in surgical specimens of human pheochromocytomas and in a human pheochromocytoma (KAT45) cell line. Eur J Pharmacol, 364:255-262
   
Makrigiannakis Margioris A, Chatzaki Α, Zoumakis E, Chrousos G, Gravanis A. (1999) The decidualizing effect of progesterone may involve direct transcriptional activation of corticotropin-releasing hormone (CRH) from human endometrial stroma cells.  Mol Hum Reprod 5:788-796
   
Athanassaki I, Farmakiotis V, Aifantis I, Gravanis A, Vassiliadis S. (1999). Expression of corticotropin-releasing hormone in the mouse uterus: participation in embryo implantation. J Endocrinol 163:221-227
   
Makrigiannakis A, Margioris A, Zoumakis E, Stournaras C, Gravanis A. (1999). The transcription of corticotropin-releasing hormone in human endometrial cells is regulated by cytokines. Neuroendocrinology 70:451-459
   
Tsapara A, Kardassis D, Moustakas A, Gravanis A, Stournaras C. (1999). Expression and characterization of Cys374 mutated human beta-actin in two different mammalian cell lines: impaired microfilament organization and stability. FEBS Lett, 4:117-122
   
Koukouritaki S, Gravanis A, Stournaras C. (1999). Tyrosine phosphorylation of focal adhesion kinase is involved in the rapid non-genomic action of dexamethasone on actin cytoskeleton Mol Medicine, 5:731-742
   
1998
   
Venihaki M, Ain KB, Dermitzaki E, Gravanis A, Margioris AN. (1998). KAT45, a noradrenergic human pheochromocytoma cell line producing corticotropin releasing hormone. Endocrinology, 139:713-722
   
Venihaki M, Gravanis A, Margioris A. (1998). KAT45 human pheochromocytoma cell line: a new model for the in vitro study of neuro-immuno-hormonal interactions. Ann NY Acad Sci, 840: 67-75
   
1997
   
Makrigiannakis A, Psychoyos A, Zoumakis E, Margioris A, Stournaras C, Gravanis A. (1997). Endometrial corticotropin-releasing hormone: expression, regulation and physiological implications. Ann NY Acad Sci, 816:116-128
   
Hassan E, Creatsas G, Gravanis A, Georgoulias V, Psychoyos A. (1997). Anti-STD’s vaginal contraceptive sponges. Ann NY Acad Sci, 816:451-456
   
Zoumakis E, Makrigiannakis A, Margioris A, Stournaras C, Gravanis A. (1997). Endometrial Corticotropin-releasing hormone: potential autocrine-paracrine actions. Ann NY Acad Sci, 828:84-95

Venihaki M, Gravanis A, Margioris AN. (1997). Comparative study between normal rat chromaffin and PC12 rat pheochromocytoma cells: production and effects of corticotropin releasing hormone. Endocrinology, 138:698-704
   
Koukouritaki S, Margioris A, Gravanis A, Hartig R, Stournaras C. (1997). Dexamethasone induces actin assembly in human endometrial cells without affecting its synthesis. J Cell Biochem, 65: 492-500
   
Markogiannakis E, Georgoulias V, Margioris A, Zoumakis E,  Stournaras C, Gravanis A. (1997). Estrogen and glucocorticoids induce the expression of erbB2/neu receptor in Ishikawa human endometrial adenocarcinoma cells. Life Sci, 61:1083-1095
   
1996
   
Karl M, Chrousos G, Saviolakis G, Gravanis A, Margioris A. (1996). The PC12 rat pheochromocytoma cell line expresses the prodynorphin gene and secretes the 8kDa dynorphin product. Regul Peptides, 61:99-104

Venihaki M, Gravanis A, Margioris A. (1996). Kappa-opioid agonists exert a naloxone-reversible effect on the proliferation of the PC12 rat pheochromocytoma cells. Peptides, 17:413-419
   
Makrigiannakis A, Zoumakis E, Margioris A, Stournaras C, Chrousos G, Gravanis A. (1996). Regulation of the promoter of human corticotropin-releasing hormone gene in transfected human endometrial cell Neuroendocrinology, 64:85-93
   
Koukouritaki E, Theodoropoulos P, Margioris A, Gravanis A, Stournaras C. (1996). Dexamethasone alters rapidly actin polymerization dynamics in human endometrial cells: evidence for non-genomic actions involving cAMP turnover. J Cell Biochem, 64:251-261
   
Hatzoglou A, Margioris A, Bakogeorgou E, Gravanis A, Castanas E. (1996). Identification and characterization of corticotropin-releasing hormone receptors in human placenta. Life Sci, 59:1871-1879
   
Stournaras C, Stiakaki E, Koukouritaki S, Kalmanti M, Fostinis Y, Gravanis A. (1996). Altered actin polymerization dynamics in various malignant cell types: evidence for differential sensitivity to Cytochalasin B. Biochem Pharmacology, 52:1339-1346
   
Papakostanti E, Emmanouel D, Gravanis A, Stournaras C. (1996). Na/Pi cotransport alters rapidly cytoskeletal protein polymerization dynamics in Opossum kidney cells. Biochem J,  315:241-247
   
1995
   
#Makrigiannakis A / #Zoumakis E, Margioris A, Theodoropoulos P, Stournaras C, Gravanis A. (1995). The Corticotropin-Releasing Hormone in normal and tumoral epithelial cells of human endometrium. J Clin Endocrinol Metab, 80:185-189 (# both authors contributed equally to this work)
   
Hatzoglou A, Gravanis A, Margioris A, Zoumakis E, Castanas E. (1995). Identification and characterization of opioid binding sites present in the Ishikawa human endometrial adenocarcinoma cell line. J Clin Endocrinol Metab, 80:418-423
   
Makrigiannakis A, Margioris A, LeGoascogne C, Zoumakis E, Nikas G,  Psychoyos A, Stournaras C, Gravanis A. (1995). Corticotropin-releasing hormone (CRH) is expressed at the implantation sites of the early pregnant rat uterus. Life Sci, 57:1869-1875
   
Venihaki M, Gravanis A, Margioris A. (1995). Opioids inhibit dopamine secretion from PC12 rat pheochromocytoma cells Life Sci, 58:75-82
   
1994
   
Courtot AM, Nikas G, Gravanis A, Psychoyos A. (1994). Effects of Cholic acid and Protectaid formulations on human sperm motility and ultrastructure. Human Reprod, 9:1999-2005
   
Theodoropoulos P, Gravanis A, Tsapara A, Margioris A, Papadogiorgaki E, Galanopoulos V, Stournaras C. (1994). Cytochalasin B may shorten  actin filaments by a mechanism independent of barbed end capping. Biochem Pharmacology, 47:1875-1881
   
Haussinger D, Sallburger C, Theodoropoulos P, Stoll B, Markogiannakis E, Gravanis A, Lang F, Stournaras C. (1994). Microtubular alterations during cell swelling in isolated rat hepatocytes. Biochem Cell Biol, 72:12-19
   
Antonakis N, Georgoulias V, Margioris A, Stournaras, Gravanis A. (1994). In vitro differential effects of the antiglucocorticoid RU486 on the release of lymphokines from normal human lymphocytes. J Steroid Biochem Mol Biol, 51:67-72
   
Katsantonis J, Tosca A, Koukouritaki S, Theodoropoulos P, Gravanis A, Stournaras C. (1994). Differences in the G/total actin ratio and the stability of microfilaments between normal and malignant human keratinocytes. Cell Biochem Func, 12:267-274
   
#Makrigiannakis A / #Zoumakis E, Margioris A, Theodoropoulos P, Stournaras C, Gravanis A. (1995). The Corticotropin-Releasing Hormone in normal and tumoral epithelial cells of human endometrium. J Clin Endocrinol Metab, 80:185-189 (# both authors contributed equally to this work)
   
Courtot AM, Nikas G, Gravanis A, Psychoyos A. (1994). Effects of Cholic acid and Protectaid formulations on human sperm motility and ultrastructure. Human Reprod, 9:1999-2005
   
Gravanis A, Makrigiannakis A,  Stournaras C, Margioris A. (1994). Interactions between steroid hormones and endometrial opioid peptides  Ann NY Acad Sci, 734:245-257
   
1993
   
Psychoyos A, Kreatsas P, Hassan D, Georgoulias V , Gravanis A. (1993). Spermicidal and antiviral properties of a new vaginal sponge (Protectaid) containing Cholic acid. Human Reprod, 8:866-869
   
1992
   
Makrigiannakis A, Margioris A, Markogiannakis M, Stournaras C, Gravanis A. (1992) Steroid hormones regulate the release of immunoreactive β-endorphin from the Ishikawa human endometrial cell line. J Clin Endocrinol Metab, 75:584-589 
   
Theodoropoulos P, Gravanis A, Saridakis G, Stournaras C. (1992) Normal and H-ras 1 oncogene transformed BRL cells show differential resistance to cytoskeletal protein inhibitors. Cell Biochem Func, 10:281-288

Fostinis G,Theodoropoulos T, Gravanis A, Stournaras C. (1992). Heat shock protein HSP90 and its association with the cytoskeleton : a morphological study. Biochem Cell Biol , 70:779-786
   
Margioris A, Markogiannakis M, Makrigiannakis A, Gravanis A. (1992) The PC12 rat pheochromocytoma cells synthesize dynorphin. Its secretion is modulated by nicotine and  nerve growth factor. Endocrinology, 131:703-709
   
Theodoropoulos P, Stournaras C, Stoll B, Markogiannakis E, Gravanis A, Lang F, Haussinger D. (1992) Hepatocyte swelling leads to rapid actin polymerization and increases actin mRNA levels. FEBS Lett, 311:241-245

1991
   
Antonakis N, Markogiannakis M, Theodoropoulou M, Georgoulias V, Stournaras C, Gravanis A. (1991) Antiglucocorticoid RU486 down regulates the expression of Interleukin 2 Receptor in normal human lymphocytes. J Steroid Biochem Mol Biol, 39:929-935
   
1989
   
Psychoyos A, Roche D, Gravanis A. (1989). Is Cholic acid responsible for embryo-toxicity of the post-receptive uterine environment? Human Reprod.4:832-834
   
1986
   
Guller S, Gravanis A, Gurpide E. (1986) Steroid metabolising enzymes associated with the microvillar membrane of Human Placenta. J. Steroid Biochem.24:935-944
   
Gravanis A, Gurpide E. (1986) .Enucleation of Human Endometrial cells: nucleo-cytoplasmic distribution of DNA Polymerase a and Estrogen Receptors. J. Steroid Biochem.24:469-474
   
Holinka C, Hata H, Gravanis A, Kuramoto H, Gurpide E. (1986) Effects of Estradiol on proliferation of Endometrial Adenocarcinoma cells (Ishikawa line). J. Steroid. Biochem.25:781-786
   
Gravanis A, Gurpide E. (1986). Effects of Estradiol on DNA Polymerase a activity in a Human Endometrial adenocarcinoma cell line (Ishikawa cells).  J. Clin. Endocrinol. Metab.63:356-359
   
1985
   
Gravanis A, Schaison G, George M, Satyaswaroop P, Baulieu EE, Robel P. (1985).Endometrial and Pituitary responses to the steroidal antiprogestin RU486 in post-menopausal women. J. Clin. Endocrinol. Metab. 60:156-163
   
1984
   
Gravanis A, Zorn JP, Tanguy G, Niessman C, Cedard L, Robel P. (1984) The dysarmonic luteal phase syndrome: Endometrial progesterone receptor and Estradiol Dehydrogenase. Fertil. Steril.42:730-736
   
Gravanis A, Binart N, Robel P, Baulieu EE, Catelli M. (1984). Estrogen-like effects of combined  Dexamethasone and Tamoxifen in the chick oviduct. Biochem. Biophys. Res. Commun.124:57-62
   
Robel P, Gravanis A, Roger L, Catelli M, Binart N, George M, Laval C, Baulieu EE. (1984).Female sex steroid receptors in post-menopausal Endometrial adenocarcinoma. Biochemical  responses to antiestrogen and progestins.  Prog.Clin.Biol.Res.142:167-179