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Venediktos Makatounakis

Venediktos Makatounakis

Research Assistant
Email address
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Phone
+30-2810-391176
Fax
+30-2810-391101
Lab web page

Representative of employees

1985:Chemistry degree from the Aristotle University of Thessaloniki.

1989-present :Research Assistant in the molecular genetics laboratory of ΙΜΒΒ(Prof. Papamatheakis and Dr. Kretsovali)

Work Experience:

Isolation and purification of nucleic acids and proteins. Plasmid constructions.

Chromatin immunoprecipitation.

Study of various transcription factors with quantitative polymerase chain reaction (Real time PCR), Confocal microscopy and Flow cytometry (FACS).

Certificated operator on MOFLO cell sorter.

Τraining of undergraduate and graduate students in molecular biology techniques.

 

The MHC class II E beta promoter: a complex arrangement of positive and negative elements determines B cell and interferon-gamma (IFN-gamma) regulated expression. Thanos D, Gregoriou M, Stravopodis D, Liapaki K, Makatounakis T, Papamatheakis J. Nucleic Acids Res. 1993 Dec 25; 21(25): 6010-6019.

Self-association of class II transactivator correlates with its intracellular localization and transactivation. Kretsovali A, Spilianakis C, Dimakopoulos A, Makatounakis T, Papamatheakis J. J Biol Chem. 2001 Aug 24;276(34)

CIITA regulates transcription onset viaSer5-phosphorylation of RNA Pol II. Spilianakis C, Kretsovali A, Agalioti T, Makatounakis T, Thanos D, Papamatheakis J. EMBO J. 2003 Oct 1;22(19):5125-36.

Interferon gamma dependent transcriptional memory via relocalization of a gene locus to PML nuclear bodies. Gialitakis M., Arampatzi P., Makatounakis T. and Papamatheakis J., (2009) Molecular and Cellular Biology 30: 2046- 2056.

Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements. Arampatzi P., Gialitakis M., Makatounakis T. and Papamatheakis J,(2012) Nucleic Acids Res 41:2202-15.

Promyelocytic Leukemia (PML) protein is an essential regulator of stem cell pluripotency and somatic cell reprogramming. C. Hadjimichael, K. Chanoumidou, C. Nikolaou, G-I Theodosi2, T. Makatounakis, J. Papamatheakis, A. Kretsovali, (2016) Stem-Cell-Reports (under revision)