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Gene Targeting Facility (GTF)

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Description

The Gene Targeting Facility offers all services associated with the production of conditional or knock-out mice from ES cells. The facility has generated chimeras from gene trap ES cells and targeted ES cells.

Equipment

The GTF is located in a separate work area within the Animal Cell Culture Facility.

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TC hood, NuAire

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TC hood, Holten

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Centrifuge, Eppendorf

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Microscope, EUROMEX

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CO2 incubator, SHEL LAB

Services

(Currently restricted to IMBB users).
•  Consultation (design of targeting vector, strategy and primers).
•  DNA purification.
•  Electroporation (R1 ES cells) picking and expanding clones.
•  DNA extraction, Southern blot analysis of ES clones.
•  Karyotyping.
•  Mycoplasma screening.
•  Defrosting, expansion of positive ES clones preparation for blastocyst injection.
•  Blastocyst injection of ES clones into blastocysts from C57BL/6 mice. Provided by the Transgenic Animal Facility.

Personnel

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Contact Person
Theodoros Kosteas
+30-2810-391072
+30-2810-391101
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A104
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Costas Kourouniotis
+30-2810-391013
+30-2810-391194
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Animal House building
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