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Gel Imaging

Description
The facility is equipped with a Storm 840 Gel and Blot Imaging System. The STORM 840 Phosphorimager is a multiuser instrument with radioactive and nonradioactive detection capabilities. Researchers can use storage phosphor screen autoradiography, direct fluorescence or chemifluorescence for their DNA, RNA, and protein samples. ImageQuant 5.2 image analysis software is used to analyze data collected by the STORM 840.

The LAS-3000 imaging system combines new CCD camera technology with a simplified user interface to provide significantly improved system sensitivity with a wide range of modularities. This system is especially suitable for Western blot analysis. High sensitive chemiluminescent detection was designed to compete with the X-ray films. Image acquire and digitize can capture both chemiluminescence and digitized marker image by one click.

A 5-position filter turret is mounted in front of the lens, which allows convenient, electrically controlled filter selection in less than ten seconds. With Image Reader Lite software, the user has control over three standard filters (605DF40 for EtBr, Y515 for SYBR® Green and 510DF10 for GFP), a fourth filter position for chemiluminescence and a fifth user-specified filter for fluorescence. With Image Reader Pro software, the user has full control over five filter types and positions. Other than the above, 575 DF20 filter was prepared for green LED and R670 filler was prepared for red LED. The number of light sources on the LAS-3000 system has been increased to six, including UV transilluminator, white transilluminator, blue epi-illuminator and white epi-illuminator, green epi-illuminator and red epi-illuminator.With Image Reader Lite software, the user has control over four light sources (UV trans-illuminator, white trans-illuminator, blue epi-illuminator, and white epi-illuminator). With Image Reader Pro software, the user has control over all the six light sources (see above, plus green epi-illuminator and red epi-illuminator).

Fujifilm LAS300 CCD camera imaging station
Storm840 Phosphorimager isotope
and fluorescence imaging station

 

Agarose gel documentation system Core facility 1
Agarose gel documentation system Core facility 2

Equipment
The Storm 840 Gel and Blot Imaging system from Molecular dynamics (GE Healthcare, formerly Amersham Biosciences) consists of a Storm 840 scanner , an Image Eraser for screens and a PC exclusively used for storage and analysis of acquired data with related software.

Storm 840 & ImageQuant TL from GE Healthcare

Large format imaging systems for filmless autoradiography, fluorescence, and chemifluorescence imaging. Filmless autoradiography based on proven PhosphorimagerT technology. Direct red- and blue-excited fluorescence enable rapid and accurate quantitation using fluorescence- and chemifluorescence-based methods. Five orders of linear dynamic range ensure accurate, publication-quality results on the first exposure.

Fuji (LAS 3000 Imager)

Contact Person (for Storm 840)

Name Niki Gounalaki
Telephone number +30-2810-391172
Email address This email address is being protected from spambots. You need JavaScript enabled to view it.
Room Number A240

Contact Person (for LAS 3000 Imager)

Name Marina Koukaki
Telephone number +30-2810-391167 (lab),
+30-6944746766 (mobile)
Email address This email address is being protected from spambots. You need JavaScript enabled to view it.
Room Number A202

 

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