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John Vontas

John Vontas

IMBB Group Leader (Associate Professor, Agr. Pharmacology)
Email address
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Phone
+30-2810-394077
Fax
+30-2810-391101
Lab web page

Some of the most acute challenges that the world faces now and in the foreseeable future are caused by insecticide resistant arthropods that seriously threaten human health and food security. Research in my laboratory focusses on the molecular analysis of the mechanisms by which insects develop resistance to insecticides, aiming to develop means of managing and overcoming this resistance. Our approaches include, classical bioassays, transcriptomic analysis and genetic studies, to identify genes and mutatons associated with resistance. By using advanced recombinant enzymes production systems and analytical techniques, as well as functional in vivo approaches, we validate the role of genes and mutations in resistance. We also look at the tissue specific expression of enzymes involved in resistance by immunolocalisation techniques, aiming to identify the major attrition points in delivery of insecticide to the target site - our most recent research on that focuses on the oenocytes of the major malaria vector Anopheles gambiae. Advanced molecular diagnostics are developed, and used to measure resistance dynamics and evolution in major disease vectors and agricultural pests. Inhibitors of detoxification enzymes for novel insecticide formulations capable to tackle resistance are developed in collaboration with the Industry. Tools for in vitro and in vivo screens of novel active ingredients and synergists (additives) are finally produced.

Balabanidou V, Kampouraki A, MacLean M, Blomquist G.J, Tittiger C, Juárez P, Mijailovsky S.J, Chalepakis G, Anthousi A, Lynde A, Antoine S, Hemingway J, Ranson H, Lycett G.J, Vontas J. (2016) Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae. PNAS doi: 10.1073/pnas.1608295113.

Grigoraki L, Balabanidou V, Meristoudis C, Miridakis A, Ranson H, Swevers L, Vontas J. (2016) Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus. Insect Biochem Mol Biol. 74:61-7.

Vontas J, Moores S, Kleinschmidt I, Ranson H, Lindsay S, Lengeler C, Hamon N, McLean T, Hemingway J. (2014) A Logical Framework for assessment and adoption of new vector control product categories. Trends in Parasitology doi: 10.1016/j.pt.2014.02.005.

Hemingway J, Vontas J, Poupardin R, Raman J, Lines J, Schwabe C, Matias A, Kleinschmidt I. (2013) Country-level operational implementation of the Global Plan for Insecticide Resistance Management. Proceedings of the National Academy of Sciences of the United States of America 110: 9397-9402.

Dermauw W, Wybouw N, Rombauts S, Menten B, Vontas J, Grbic M, Clark RM, Feyereisen R, Van Leeuwen T. (2013) A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae. Proceedings of the National Academy of Sciences of the United States of America 110: E113-E122.

2017

(104) Fotakis M, Chaskopoulou A, Grigoraki L, Tsiamantas A, Kounadi S, Georgiou L, Vontas J. (2017) Analysis of population structure and insecticide resistance in mosquitoes of the genus Culex, Anopheles and Aedes from different environments of Greece with a history of mosquito borne disease transmission. Acta Trop. 2017 Jun 9. pii: S0001-706X(17)30356-X. doi: 10.1016/j.actatropica.2017.06.005. PMID: 28606820

(103) Santos-Matos G, Wybouw N, Martins NE, Zélé F, Riga M, Leitão AB, Vontas J, Grbić M, Van Leeuwen T, Magalhães S, Sucena É. (2017) Tetranychus urticae mites do not mount an induced immune response against bacteria. Proc Biol Sci. 2017 Jun 14;284(1856). pii: 20170401. doi: 10.1098/rspb.2017.0401. PMID: 28592670

(102) Grigoraki L, Balabanidou V, Meristoudis C, Miridakis A, Ranson H, Swevers L, Vontas J. (2017) Corrigendum to "Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus" [Insect Biochem. Mol. Biol. 74 (July 2016) 61-67]. Insect Biochem Mol Biol. 85:32. doi: 10.1016/j.ibmb.2017.05.003 PMID: 28578775

(101) Corbel V, Fonseca DM, Weetman D, Pinto J, Achee NL, Chandre F, Coulibaly MB, Dusfour I, Grieco J, Juntarajumnong W, Lenhart A, Martins AJ, Moyes C, Ng LC, Raghavendra K, Vatandoost H, Vontas J, Muller P, Kasai S, Fouque F, Velayudhan R, Durot C, David JP. (2017) International workshop on insecticide resistance in vectors of arboviruses, December 2016, Rio de Janeiro, Brazil. Parasit Vectors. 10(1):278. doi: 10.1186/s13071-017-2224-3. PMID: 28577363

(100) Blow F, Vontas J, Darby AC. (2017) Draft Genome Sequence of Chryseobacterium Strain CBo1 Isolated from Bactrocera oleae. Genome Announc. 5(18). pii: e00177-17. doi: 10.1128/genomeA.00177-17. PMID: 28473371

(99) Grigoraki L, Pipini D, Labbé P, Chaskopoulou A, Weill M, Vontas J. (2017) Carboxylesterase gene amplifications associated with insecticide resistance in Aedes albopictus: Geographical distribution and evolutionary origin. PLoS Negl Trop Dis.11(4):e0005533. doi: 10.1371/journal.pntd.0005533. PMID: 28394886

(98) Pavlidi N, Gioti A, Wybouw N, Dermauw W, Ben-Yosef M, Yuval B, Jurkevich E, Kampouraki A, Van Leeuwen T, Vontas J. (2017) Transcriptomic responses of the olive fruit fly Bactrocera oleae and its symbiont Candidatus Erwinia dacicola to olive feeding. Sci Rep. 7:42633. doi: 10.1038/srep42633. PMID: 28225009

(97) Aris Ilias, Vassilis A. Vassiliou, John Vontas, Anastasia Tsagkarakou (2016) Molecular diagnostics for detecting pyrethroid and abamectin resistance mutations in Tetranychus urticae. Pesticide Biochemistry and Physiology 135:9-14. doi: 10.1016/j.pestbp.2016.07.004 online PMID: 28043338

2016

(96) Longdon B., Day J.P., Schulz N., Leftwich P.T., de Jong M.A., Breuker C.J., Gibbs M., Obbard D.J., Wilfert L., Smith S.CL., McGonigle J.E., Houslay T.M., Wright L., Livraghi L., Evans L.C., Friend L.A, Chapman T., Vontas J., Kambouraki N., Jiggins F.M. (2016) Vertical transmission and population dynamics of three insect rhabdoviruses. Proceedings B doi: http://dx.doi.org/10.1101/084558

(95) Stilianakis NI, Syrris V, Petroliagkis T, Pärt P, Gewehr S, Kalaitzopoulou S, Mourelatos S, Baka A, Pervanidou D, Vontas J, Hadjichristodoulou C. (2016) Identification of Climatic Factors Affecting the Epidemiology of Human West Nile Virus Infections in Northern Greece.PLoS One. 11(9):e0161510. doi: 10.1371/journal.pone.0161510. PMID: 27631082

(94) Vasileia Balabanidou, Anastasia Kampouraki, Marina MacLean, Gary J. Blomquist, Claus Tittiger,M. Patricia Juárez, Sergio J. Mijailovsky, George Chalepakis, Amalia Anthousi, Amy Lynde, Sanou Antoine, Janet Hemingway, Hilary Ranson, Gareth J. Lycett, John Vontas (2016) Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae. PNAS doi: 10.1073/pnas.1608295113 PMID: 27439866

(93) Grigoraki L, Balabanidou V, Meristoudis C, Miridakis A, Ranson H, Swevers L, Vontas J (2016) Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus. Insect Biochem Mol Biol. 2016 Jul;74:61-7. doi: 10.1016/j.ibmb.2016.05.007 PMID: 27180726

2015

(92) Chen X., Jiang X., Gu J., Xu M., Wu Y., Deng Y., Zhang C., Bonizzoni M., Dermauw W., Vontas J., Armbruster P., Huang X., Yang Y., Hao Z., He W., Peng H., Liu Y., Wu K., Chen J., Lirakis M., Topalis P., Van Leeuwen T., Hall AB., Jiang X., Thorpe C., Mueller RL., Sun C., Waterhouse RM.,Yan G., Tu ZJ., Fang X., James AA. (2015) The genome sequence of the Asian Tiger mosquito, Aedes albopictus reveals insights into its biology, genetics and evolution. PNAS (in press). doi: 10.1073/pnas.1516410112 PMID: 26483478

(91) Grigoraki L., Lagnel J., Kioulos I., Kampouraki A., Morou E., Labbé P., Weill M., Vontas J. (2015) Transcriptome profiling and genetic study reveal amplified carboxylesterase genes implicated in temephos resistance, in the Asian tiger mosquito Aedes albopictus. PloS Negl Trop Diseases. 9(5):e0003771. doi: 10.1371/journal.pntd.0003771. PMID: 26000638

(90) Riga M., Myridakis ., Tsakireli ., Morou ., Stephanou ., Nauen ., Van Leeuwen ., Douris V., Vontas J. (2015) Functional characterization of the Tetranychus urticae CYP392A11, a cytochrome P450 that hydroxylates the METI acaricides cyenopyrafen and fenpyroximate. Insect Biochemistry and Molecular Biology. 65: 91-99. doi: 10.1016/j.ibmb.2015.09.004. PMID: 26363294

(89) Nauen R., Wölfel K., Lueke B., Myridakis A., Tsakireli D, Roditakis E., Tsagkarakou A, Stephanou E., Vontas J. (2015) Development of a lateral flow test to detect metabolic resistance in Bemisia tabaci mediated by CYP6CM1, a cytochrome P450 with broad spectrum catalytic efficiency. Pesticide Biochemistry and Physiology. 121: 3-11. doi:10.1016/j.pestbp.2014.12.023. PMID: 26047106

(88) Pavlidi N., Tseliou V., Riga M., Nauen R., Van Leeuwen T., Labrou N. and Vontas J. (2015) Functional characterization of glutathione S-transferases associated with insecticide resistance in Tetranychus urticae. Pesticide Biochemistry and Physiology. 121: 53-60. doi:10.1016/j.pestbp.2015.01.009. PMID: 26047112

(87) Thawer N.G., Ngondi J., Mugalura F., Mwalimu D., Morou E., Vontas J., Protopopoff N., Mutagahywa J., Lalji S., Molteni F., Ramsan M., Willilo R., Kafuko J., Ndong I., Reithinger R., Magesa S. (2015) Use of Insecticide Quantification Kits to investigate the quality of spraying and decay rate of Bendiocarb on different wall surfaces in Kagera region, Tanzania. Parasites and Vectors. 8: 242. doi: 10.1186/s13071-015-0859-5. PMID: 25896604

(86) Jones C.M, Papanicolaou A, Mironidis G.K, Vontas J, Yang Y., Lim K.S., Oakeshott J.G., Chris Bass C., Chapman J.W. (2015) Genome-wide transcriptional signatures of migratory flight activity in a globally invasive insect pest. Molecular Ecology. doi: 10.1111/mec.13362. Β [Epub ahead of print] PMID: 26331997

(85) Nauen R., Wölfel K., Lueke B., Myridakis A., Tsakireli D, Roditakis E., Tsagkarakou A, Stephanou E., Vontas J. (2015) Development of a lateral flow test to detect metabolic resistance in Bemisia tabaci mediated by CYP6CM1, a cytochrome P450 with broad spectrum catalytic efficiency. Pesticide Biochemistry and Physiology. 121: 3-11. doi:10.1016/j.pestbp.2014.12.023. PMID: 26047106

(84) Pavlidi N., Tseliou V., Riga M., Nauen R., Van Leeuwen T., Labrou N. and Vontas J. (2015) Functional characterization of glutathione S-transferases associated with insecticide resistance in Tetranychus urticae. Pesticide Biochemistry and Physiology. 121: 53-60. doi:10.1016/j.pestbp.2015.01.009. PMID: 26047112

(83) Balabanidou V., Kambouraki N., Chalepakis G., Ranson H., Lyccett G., and Vontas J. (2015) The 4G cytochrome P450s in the malaria mosquito Anopheles gambiae (submitted)

(82) Thawer N.G., Ngondi J., Mugalura F., Mwalimu D., Morou E., Vontas J., Protopopoff N., Mutagahywa J., Lalji S., Molteni F., Ramsan M., Willilo R., Kafuko J., Ndong I., Reithinger R., Magesa S. (2015) Use of Insecticide Quantification Kits to investigate the quality of spraying and decay rate of Bendiocarb on different wall surfaces in Kagera region, Tanzania. Parasites and Vectors. 8: 242. doi: 10.1186/s13071-015-0859-5. PMID: 25896604

2014

(81) Leftwich P., Koukidou M., Rempoulakis C, Gong HF, Zacharopoulou A., Chapman T., Economopoulos A., Vontas J., & Alphey L. (2014) Genetic Control of the Mediterranean Fruit Fly Proc. R. Soc. B 281: 20141372. http://dx.doi.org/10.1098/rspb.2014.1372.

(80) Neafsey D., Robert M. Waterhouse R., ...,Vontas J.,..., Zwiebel L., Besansky N (2014) Highly evolvable malaria vectors: The genomes of 16 Anophelesmosquitoes Published Online Science (early edition) Science DOI: 10.1126/science.1258522.

(79) Ingham, Jonew CM,V.A., Pignatelli P,, Balabanidou V, Vontas J, Wagstaff SC, Moore JD, Ranson H.. Dissecting the organ specificity of insecticide resistance candidate genes in Anopheles gambiae: known and novel candidate genes. BMC Genomics, 2014. 15(1): p. 1018.

(78) Ilias A, J Vontas, and A Tsagkarakou (2014) Global distribution and origin of target site insecticide resistance mutations in Tetranychus urticae. Insect Biochemistry and Molecular Biology 48: 17-28.

(77) Kioulos I, A Kampouraki, E Morou, G Skavdis, and J Vontas (2014) Insecticide resistance status in the major West Nile virus vector Culex pipiens from Greece.Pest Management Science 70: 623-627.

(76) Riga M, D Tsakireli, A Ilias, E Morou, A Myridakis, EG Stephanou, R Nauen, W Dermauw, T Van Leeuwen, M Paine, and J Vontas (2014) Abamectin is metabolized by CYP392A16, a cytochrome P450 associated with high levels of acaricide resistance in Tetranychus urticae. Insect Biochemistry and Molecular Biology 46: 43-53.

(75) Roditakis E, N Fytrou, M Staurakaki, J Vontas, and A Tsagkarakou (2014) Activity of flonicamid on the sweet potato whitely Bemisia tabaci (Homoptera: Aleyrodidae) and its natural enemies. Pest Management Science: doi: 10.1002/ps.3723.

(74) Vontas J, S Moores, I Kleinschmidt, H Ranson, S Lindsay, C Lengeler, N Hamon, T McLean, and J Hemingway (2014) A Logical Framework for assessment and adoption of new vector control product categories. Trends in Parasitology: doi: 10.1016/j.pt.2014.02.005.

(73) Bempelou, E.D., Vontas, J., Liapis, K.S., Ziogas, V.N. (2013) Biodegradation of diazinon by the epiphytic yeasts Rhodotorula glutinis and Rhodotorula rubra.Hellenic Plant Protection Journal Volume 6, Issue 2, 2013, Pages 69-82.

2013

(72) Hemingway J, J Vontas, R Poupardin, J Raman, J Lines, C Schwabe, A Matias, and I Kleinschmidt (2013) Country-level operational implementation of the Global Plan for Insecticide Resistance Management. Proceedings of the National Academy of Sciences of the United States of America 110: 9397-9402.

(71) Dermauw W, N Wybouw, S Rombauts, B Menten, J Vontas, M Grbic, RM Clark, R Feyereisen, and T Van Leeuwen (2013) A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae. Proceedings of the National Academy of Sciences of the United States of America 110: E113-E122.

(70) Demaeght P, W Dermauw, D Tsakireli, J Khajehali, R Nauen, L Tirry, J Vontas, P Lummen, and T Van Leeuwen (2013) Molecular analysis of resistance to acaricidal spirocyclic tetronic acids in Tetranychus urticae: CYP392E10 metabolizes spirodiclofen, but not its corresponding enol. Insect Biochemistry and Molecular Biology 43: 544-554.

(69) Gomes B, E Kioulos, A Papa, APG Almeida, J Vontas, and J Pinto (2013) Distribution and hybridization of Culex pipiens forms in Greece during the West Nile virus outbreak of 2010. Infection Genetics and Evolution 16: 218-225.

(68) Mironidis GK, D Kapantaidaki, M Bentila, E Morou, M Savopoulou-Soultani, and J Vontas (2013) Resurgence of the cotton bollworm Helicoverpa armigera in northern Greece associated with insecticide resistance. Insect Science 20: 505-512.

(67) Morou E, M Lirakis, N Pavlidi, M Zotti, Y Nakagawa, G Smagghe, J Vontas, and L Swevers (2013) A new dibenzoylhydrazine with insecticidal activity against Anopheles mosquito larvae. Pest Management Science 69: 827-833.

(66) Nauen R, J Vontas, M Kaussmann, and K Wolfel (2013) Pymetrozine is hydroxylated by CYP6CM1, a cytochrome P450 conferring neonicotinoid resistance in Bemisia tabaci. Pest Management Science 69: 457-461.

(65) Papadakis G, A Tsortos, A Kordas, I Tiniakou, E Morou, J Vontas, D Kardassis, and E Gizeli (2013) Acoustic detection of DNA conformation in genetic assays combined with PCR. Scientific Reports 3.

(64) Pavlidi N, W Dermauw, S Rombauts, A Chrisargiris, T Van Leeuwen, and J Vontas (2013) Analysis of the Olive Fruit Fly Bactrocera oleae Transcriptome and Phylogenetic Classification of the Major Detoxification Gene Families. Plos One 8.

2012

(63) Ant T, M Koukidou, P Rempoulakis, HF Gong, A Economopoulos, J Vontas, and L Alphey (2012) Control of the olive fruit fly using genetics-enhanced sterile insect technique. Bmc Biology 10.

(62) Bariami V, CM Jones, R Poupardin, J Vontas, and H Ranson (2012) Gene Amplification, ABC Transporters and Cytochrome P450s: Unraveling the Molecular Basis of Pyrethroid Resistance in the Dengue Vector, Aedes aegypti. Plos Neglected Tropical Diseases 6.

(61) Dermauw W, A Ilias, M Riga, A Tsagkarakou, M Grbic, L Tirry, T Van Leeuwen, and J Vontas (2012) The cys-loop ligand-gated ion channel gene family of Tetranychus urticae: Implications for acaricide toxicology and a novel mutation associated with abamectin resistance. Insect Biochemistry and Molecular Biology 42: 455-465.

(60) Doukas EG, AN Markoglou, JG Vontas, and BN Ziogas (2012) Effect of DMI-resistance mechanisms on cross-resistance patterns, fitness parameters and aflatoxin production in Aspergillus parasiticus Speare. Fungal Genetics and Biology 49: 792-801.

(59) Hsu JC, HT Feng, WJ Wu, SM Geib, CH Mao, and J Vontas (2012) Truncated transcripts of nicotinic acetylcholine subunit gene Bd alpha 6 are associated with spinosad resistance in Bactrocera dorsalis. Insect Biochemistry and Molecular Biology 42: 806-815.

(58) Ilias A, E Roditakis, M Grispou, R Nauen, J Vontas, and A Tsagkarakou (2012) Efficacy of ketoenols on insecticide resistant field populations of two-spotted spider mite Tetranychus urticae and sweet potato whitefly Bemisia tabaci from Greece. Crop Protection 42: 305-311.

(57) Kalajdzic P, S Oehler, M Reczko, N Pavlidi, J Vontas, AG Hatzigeorgiou, and C Savakis (2012) Use of Mutagenesis, Genetic Mapping and Next Generation Transcriptomics to Investigate Insecticide Resistance Mechanisms. Plos One 7.

(56) Pavlidi N, M Monastirioti, P Daborn, I Livadaras, T Van Leeuwen, and J Vontas (2012) Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster. Pesticide Biochemistry and Physiology 104: 132-135.

(55) Vontas J, E Kioulos, N Pavlidi, E Morou, A della Torre, and H Ranson (2012) Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti. Pesticide Biochemistry and Physiology 104: 126-131.

(54) Wybouw N, V Balabanidou, DJ Ballhorn, W Dermauw, M Grbic, J Vontas, and T Van Leeuwen (2012) A horizontally transferred cyanase gene in the spider mite Tetranychus urticae is involved in cyanate metabolism and is differentially expressed upon host plant change. Insect Biochemistry and Molecular Biology 42: 881-889.

2011

(53) Malandrakis AA, AN Markoglou, DC Nikou, JG Vontas, and BN Ziogas (2011) Molecular diagnostic for detecting the cytochrome b G143S-QoI resistance mutation in Cercospora beticola. Pesticide Biochemistry and Physiology 100: 87-92.

(52) Roditakis E, E Morou, A Tsagkarakou, M Riga, R Nauen, M Paine, S Morin, and J Vontas (2011) Assessment of the Bemisia tabaci CYP6CM1vQ transcript and protein levels in laboratory and field-derived imidacloprid-resistant insects and cross-metabolism potential of the recombinant enzyme. Insect Science 18: 23-29.

(51) Vassiliou V, M Emmanouilidou, A Perrakis, E Morou, J Vontas, A Tsagkarakou, and E Roditakis (2011) Insecticide resistance in Bemisia tabaci from Cyprus.Insect Science 18: 30-39.

(50) Vontas J, P Hernandez-Crespo, JT Margaritopoulos, F Ortego, HT Feng, KD Mathiopoulos, and JC Hsu (2011) Insecticide resistance in Tephritid flies. Pesticide Biochemistry and Physiology 100: 199-205.

(49) Voudouris CC, B Sauphanor, P Franck, M Reyes, Z Mamuris, JA Tsitsipis, J Vontas, and JT Margaritopoulos (2011) Insecticide resistance status of the codling moth Cydia pomonella (Lepidoptera: Tortricidae) from Greece. Pesticide Biochemistry and Physiology 100: 229-238.

2010

(48) Bass C., Nikou D., Vontas J., Donnelly M.J., Williamson M.S. Field L.M. (2010) The Vector Population Monitoring Tool (VPMT): High-throughput DNA-based diagnostics for the monitoring of mosquito vector populations. Malaria Research and Treatment ID 190434, 8 pages doi:10.4061/2010/190434.

(47) Bass C, D Nikou, J Vontas, MS Williamson, and LM Field (2010) Development of high-throughput real-time PCR assays for the identification of insensitive acetylcholinesterase (ace-1(R)) in Anopheles gambiae. Pesticide Biochemistry and Physiology 96: 80-85.

(46) Dowd AJ, E Morou, A Steven, HM Ismail, N Labrou, J Hemingway, MJI Paine, and J Vontas (2010) Development of a colourimetric pH assay for the quantification of pyrethroids based on glutathione-S-transferase. International Journal of Environmental Analytical Chemistry 90: 922-933.

(45) Khajehali J, T Van Leeuwen, M Grispou, E Morou, H Alout, M Weill, L Tirry, J Vontas, and A Tsagkarakou (2010) Acetylcholinesterase point mutations in European strains of Tetranychus urticae (Acari: Tetranychidae) resistant to organophosphates. Pest Management Science 66: 220-228.

(44) Morou E, AJ Dowd, S Rajatileka, A Steven, J Hemingway, H Ranson, M Paine, and J Vontas (2010) A Simple Colorimetric Assay for Specific Detection of Glutathione-S Transferase Activity Associated with DDT Resistance in Mosquitoes. Plos Neglected Tropical Diseases 4.

(43) Poupardin R, MA Riaz, J Vontas, JP David, and S Reynaud (2010) Transcription profiling of eleven cytochrome P450s potentially involved in xenobiotic metabolism in the mosquito Aedes aegypti. Insect Molecular Biology 19: 185-193.

(42) Van Leeuwen T, J Vontas, A Tsagkarakou, W Dermauw, and L Tirry (2010) Acaricide resistance mechanisms in the two-spotted spider mite Tetranychus urticae and other important Acari: A review. Insect Biochemistry and Molecular Biology 40: 563-572.

(41) Vontas J, H Ranson, and L Alphey (2010) Transcriptomics and disease vector control. Bmc Biology 8.

2009

(40) Dialynas E, P Topalis, J Vontas, and C Louis (2009) MIRO and IRbase: IT Tools for the Epidemiological Monitoring of Insecticide Resistance in Mosquito Disease Vectors. Plos Neglected Tropical Diseases 3.

(39) Dowd AJ, A Steven, E Morou, J Hemingway, J Vontas, and MJI Paine (2009) A simple glutathione transferase-based colorimetric endpoint assay for insecticide detection. Enzyme and Microbial Technology 45: 164-168.

(38) Karunker I, E Morou, D Nikou, R Nauen, R Sertchook, BJ Stevenson, MJI Paine, S Morin, and J Vontas (2009) Structural model and functional characterization of the Bemisia tabaci CYP6CM1vQ, a cytochrome P450 associated with high levels of imidacloprid resistance. Insect Biochemistry and Molecular Biology39: 697-706.

(37) Kazanidou A, D Nikou, M Grigoriou, J Vontas, and G Skavdis (2009) Short Report: A Multiplex PCR Assay for Simultaneous Genotyping of kdr and ace-1 Loci in Anopheles gambiae. American Journal of Tropical Medicine and Hygiene 80: 236-238.

(36) Nikou D, A Malandrakis, M Konstantakaki, J Vontas, A Markoglou, and B Ziogas (2009) Molecular characterization and detection of overexpressed C-14 alpha-demethylase-based DMI resistance in Cercospora beticola field isolates. Pesticide Biochemistry and Physiology 95: 18-27.

(35) Roditakis E, M Grispou, E Morou, JB Kristoffersen, N Roditakis, R Nauen, J Vontas, and A Tsagkarakou (2009) Current status of insecticide resistance in Q biotype Bemisia tabaci populations from Crete. Pest Management Science 65: 313-322.

(34) Tsagkarakou A, D Nikou, E Roditakis, M Sharvit, S Morin, and J Vontas (2009) Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae). Pesticide Biochemistry and Physiology 94: 49-54.

(33) Tsagkarakou A, T Van Leeuwen, J Khajehali, A Ilias, M Grispou, MS Williamson, L Tirry, and J Vontas (2009) Identification of pyrethroid resistance associated mutations in the para sodium channel of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae). Insect Molecular Biology 18: 583-593.

(32) Ziogas BN, D Nikou, AN Markoglou, AA Malandrakis, and J Vontas (2009) Identification of a novel point mutation in the beta-tubulin gene of Botrytis cinerea and detection of benzimidazole resistance by a diagnostic PCR-RFLP assay. European Journal of Plant Pathology 125: 97-107.

2008

(31) Bass C, D Nikou, AM Blagborough, J Vontas, RE Sinden, MS Williamson, and LM Field (2008) PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods. Malaria Journal 7.

(30) Kapoli P, IA Axarli, D Platis, M Fragoulaki, M Paine, J Hemingway, J Vontas, and NE Labrou (2008) Engineering sensitive glutathione transferase for the detection of xenobiotics. Biosensors & Bioelectronics 24: 498-503.

(29) Karunker I, J Benting, B Lueke, T Ponge, R Nauen, E Roditakis, J Vontas, K Gorman, I Denholm, and S Morin (2008) Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera : Aleyrodidae). Insect Biochemistry and Molecular Biology 38: 634-644.

(28) Margaritopoulos JT, G Skavdis, N Kalogiannis, D Nikou, E Morou, PJ Skouras, JA Tsitsipis, and J Vontas (2008) Efficacy of the pyrethroid alpha-cypermethrin against Bactrocera oleae populations from Greece, and improved diagnostic for an iAChE mutation. Pest Management Science 64: 900-908.

(27) McLaughlin LA, U Niazi, J Bibby, JP David, J Vontas, J Hemingway, H Ranson, MJ Sutcliffe, and MJI Paine (2008) Characterization of inhibitors and substrates of Anopheles gambiae CYP6Z2. Insect Molecular Biology 17: 125-135.

(26) Morou E, HM Ismail, AJ Dowd, J Hemingway, N Labrou, M Paine, and J Vontas (2008) A dehydrochlorinase-based pH change assay for determination of DDT in sprayed surfaces. Analytical Biochemistry 378: 60-64.

(25) Poupardin R, S Reynaud, C Strode, H Ranson, J Vontas, and JP David (2008) Cross-induction of detoxification genes by environmental xenobiotics and insecticides in the mosquito Aedes aegypti: Impact on larval tolerance to chemical insecticides. Insect Biochemistry and Molecular Biology 38: 540-551.

2007

(24) Bass C, D Nikou, MJ Donnelly, MS Williamson, H Ranson, A Ball, J Vontas, and LM Field (2007) Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods. Malaria Journal 6.

(23) Black WC and JG Vontas (2007) Affordable assays for genotyping single nucleotide polymorphisms in insects. Insect Molecular Biology 16: 377-387.

(22) Vontas J, JP David, D Nikou, J Hemingway, GK Christophides, C Louis, and H Ranson (2007) Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization. Insect Molecular Biology 16: 315-324.

2006

(21) Malandrakis AA, AN Markoglou, DC Nikou, JG Vontas, and BN Ziogas (2006) Biological and molecular characterization of laboratory mutants of Cercospora beticola resistant to Qo inhibitors. European Journal of Plant Pathology 116: 155-166.

(20) Nardi F, A Carapelli, JG Vontas, R Dallai, GK Roderick, and F Frati (2006) Geographical distribution and evolutionary history of organophosphate-resistant Ace alleles in the olive fly (Bactrocera oleae). Insect Biochemistry and Molecular Biology 36: 593-602.

(19) Roditakis E, A Tsagkarakou, and J Vontas (2006) Identification of mutations in the para sodium channel of Bemisia tabaci from Crete, associated with resistance to pyrethroids. Pesticide Biochemistry and Physiology 85: 161-166.

2005

(18) David JP, C Strode, J Vontas, D Nikou, A Vaughan, PM Pignatelli, C Louis, J Hemingway, and H Ranson (2005) The Anopheles gambiae detoxification chip: A highly specific microarray to study metabolic-based insecticide resistance in malaria vectors. Proceedings of the National Academy of Sciences of the United States of America 102: 4080-4084.

(17) Hawkes NJ, RW Janes, J Hemingway, and J Vontas (2005) Detection of resistance-associated point mutations of organophosphate-insensitive acetylcholinesterase in the olive fruit fly, Bactrocera oleae (Gmelin). Pesticide Biochemistry and Physiology 81: 154-163.

(16) Kotsyfakis M, J Vontas, I Siden-Kiamos, and C Louis (2005) The annexin gene family in the malaria mosquito Anopheles gambiae. Insect Molecular Biology 14: 555-562.

(15) Vontas J, C Blass, AC Koutsos, JP David, FC Kafatos, C Louis, J Hemingway, GK Christophides, and H Ranson (2005) Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure. Insect Molecular Biology 14: 509-521.

(14) Che-Mendoza A,. Penilla-Navarro P.R., Martinez-Barnetche J., Rodriguez-Ramirez A., Hemingway J, Vontas J (2007) Cloning and sequencing of the probable gene responsible of the DDT resistance in An albimanus (Diptera, Culicidae). Entomol. Mex. 6: 894-898.

(13) Vontas J, I Siden-Kiamos, G Papagiannakis, M Karras, AP Waters, and C Louis (2005) Gene expression in Plasmodium berghei ookinetes and early oocysts in a co-culture system with mosquito cells. Molecular and Biochemical Parasitology 139: 1-13.

2004

(12) Vontas JG, L McCarroll, S Karunaratne, C Louis, H Hurd, and J Hemingway (2004) Does environmental stress affect insect-vectored parasite transmission?Physiological Entomology 29: 210-213.

2003

(11) Ortelli F, LC Rossiter, J Vontas, H Ranson, and J Hemingway (2003) Heterologous expression of four glutathione transferase genes genetically linked to a major insecticide-resistance locus from the malaria vector Anopheles gambiae. Biochemical Journal 373: 957-963.

2002

(10) Coleman M, JG Vontas, and J Hemingway (2002) Molecular characterization of the amplified aldehyde oxidase from insecticide resistant Culex quinquefasciatus.European Journal of Biochemistry 269: 768-779.

(9) Hemingway J, L Field, and J Vontas (2002) An overview of insecticide resistance. Science 298: 96-97.

(8) Vontas JG, MJ Hejazi, NJ Hawkes, N Cosmidis, M Loukas, and J Hemingway (2002) Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae. Insect Molecular Biology 11: 329-336.

(7) Vontas JG, GJ Small, DC Nikou, H Ranson, and J Hemingway (2002) Purification, molecular cloning and heterologous expression of a glutathione S-transferase involved in insecticide resistance from the rice brown planthopper, Nilaparvata lugens. Biochemical Journal 362: 329-337.

2001

(6) Enayati AA, JG Vontas, GJ Small, L McCarroll, and J Hemingway (2001) Quantification of pyrethroid insecticides from treated bednets using a mosquito recombinant glutathione S-transferase. Medical and Veterinary Entomology 15: 58-63.

(5) Vontas JG, N Cosmidis, M Loukas, S Tsakas, MJ Hejazi, A Ayoutanti, and J Hemingway (2001) Altered acetylcholinesterase confers organophosphate resistance in the olive fruit fly Bactrocera oleae. Pesticide Biochemistry and Physiology 71: 124-132.

(4) Vontas JG, GJ Small, and J Hemingway (2001) Glutathione S-transferases as antioxidant defence agents confer pyrethroid resistance in Nilaparvata lugens.Biochemical Journal 357: 65-72.

(3) Vontas JG, SC Tsakas, M Loukas, and J Hemingway (2001) Low-activity allele of copper-zinc superoxide dismutase (CuZnSOD) in Drosophila increases paraquat genotoxicity but does not affect near UV radiation damage. Genome 44: 597-601.

2000

(2) Vontas JG, AA Enayati, GJ Small, and J Hemingway (2000) A simple biochemical assay for glutathione S-transferase activity and its possible field application for screening glutathione S-transferase-based insecticide resistance. Pesticide Biochemistry and Physiology 68: 184-192.

(1) Vontas JG, GJ Small, and J Hemingway (2000) Comparison of esterase gene amplification, gene expression and esterase activity in insecticide susceptible and resistant strains of the brown planthopper, Nilaparvata lugens (Stal). Insect Molecular Biology 9: 655-660.

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