Description
Biosensors lab, equipped with commercially available optical and acoustic sensing instruments as well as home-made SAW and hybrid SPR/SAW-based measuring systems, are set for performing real time analysis of biomolecular interactions. Studied interactions include protein-protein, cellular receptor/ligand and DNA/DNA or DNA/protein systems. Information obtained includes affinity and kinetic parameters of the binding reaction, thickness of bound bio-film and information on the conformation of bound molecules. In addition to the above surface-sensitive techniques, solution-based biomolecular interactions are studied using standard biochemical fluorescent and colorimetric assays.

Equipment

Surface Plasmon Resonance Refractometer SR7000
Reichert Analytical Instruments

Quartz Crystal Microbalance Sensor Q-Sense D300
Q-Sense

Surface plasma cleaner/sterilizer
Harrick

Multi-mode microplate reader Synergy HT
BIO-TEK

Services
The facility can provide researchers with:
•  Immobilization strategies for biomolecules or other polymers
•  Binding studies and determination of kinetic parameters for biomolecular interactions
•  Conformational or mechanical studies of biomolecules (DNA, proteins, lipids) in real-time
•  Adhesion studies for cells
•  Time-resolved fluorimetric studies

In order to use the facility for studying specific binding systems, please contact the following person.

Contact Person

Links (Publications)
For full list of publications please visit the site below:
http://www.imbb.forth.gr/people/gizeli/index.html

Biosensors -Biophysical Studies:
G. Papadakis, A. Tsortos, K. Mitsakakis, E. Gizeli,
Characterization of DNA-Hv1 histone interactions; discrimination of DNA size and shape
FEBS Letters 584: 935-940, 2010 (DNA molecules)

A. Tsortos, G. Papadakis, E. Gizeli
Shear acoustic wave biosensor for detecting DNA intrinsic viscosity
and conformation: a study with QCM-D
Biosensors and Bioelectronics 24: 842-847, 2008 (DNA molecules)

A. Tsortos/G. Papadakis, K. Mitsakakis, K. Melzak, E. Gizeli
Quantitative determination of size and shape of surface-bound DNA using an acoustic wave sensor
Biophysical Journal 97: 2706-2715, 2008 (DNA molecules)

Melzak K. Tsortos A. Gizeli E.,
Use of acoustic sensors to probe the mechanical properties of liposomes
Methods in Enzymology 465: 21-41, 2009 (Liposomes)

M. Saitakis, A. Dellaporta, E. Gizeli
Measurement of Two-Dimensional Binding Constants between Cell-Bound
Major Histocompatibility Complex and Immobilized Antibodies with an Acoustic Biosensor
Biophysical Journal 95: 4963-4971, 2008 (Cells)

K. Saha, F. Bender, E. Gizeli
Comparative study of IgG binding to proteins G and A: non-equilibrium kinetic and binding constant determination with the acoustic waveguide device
Analytical Chemistry 75: 835-842, 2003 (Proteins)

Fluorimetric studies:
C. Hadjicharalambous, T. Sheynis, R. Jelinek, M.T. Shanahan, A.J. Ouellette, E. Gizeli
Mechanisms of α -Defensin Bactericidal Action: Comparative Membrane
Disruption by Cryptdin-4 and Its Disulfide-Null Analogue
Biochemistry , 47 (47): 12626-12634, 2008

Polymer studies:
T. Shahal, K.A. Melzak, C.R. Lowe, E. Gizeli
Poly(dimethylsiloxane)-coated sensor devices for the formation of supported lipid bilayers
and the subsequent study of membrane interactions
Langmuir 24: 11268-11275, 2008

T. Drakakis, G. Papadakis, K. Sambani, G. Filippidis, S. Georgiou, E. Gizeli,
C. Fotakis, M. Farsari
Construction of three dimensional biomolecule structures employing femtosecond lasers, Applied Physics Letter 89 (1) No 144108, 2006