Description
IMBB maintains a Fermentation Facility (FF) that focuses on controlled production of biomass (mainly microbial) over a wide range of controlled conditions (temperature, pH, aeration, stirring, etc). The Fermentation Facility was established in 1988 by V. Bouriotis and houses all equipment needed for the maintenance, growth, collection and storage of microorganisms (bacteria, fungi, etc). Cell mass is produced according to strict quality criteria ( ISO90001¨2000 from TUV HELLAS ) and is accompanied from a Specifications and Technical data sheet that includes all relevant information on growth conditions. The facility complies with all the EU regulations regarding biohazard material handling and disposal. Most of the consumables used in bacterial cell cultures are disposable and the non-disposable ones are autoclaved regularly. The liquid waste are collected in vessels containing chlorine and disposed in two special sinks.

The facility provides fermentation service for:
1. The production of Restriction Enzymes, DNA/RNA Modifying Enzymes and Molecular Biology Reagents that are commercialized by Minotech biotechnology who is the main user of the facility.
2. Growth of cells for IMBB researchers for use in crystallography, membrane preparation and recombinant protein production
3. Growth of cells for the wider scientific/academic community of Crete (and Greece ).

The FF is located on the ground floor of the IMBB building (Rooms A031, A033, A034, A035).

Equipment

EQUIPMENT	COMPANY	ROOM	IMAGE	Person Responsible
Laminar Flow Hood	Bioblock Scientific	A033		D. Dialektakis
Fermentor 50 liters (35 liters working capacity)	Bioengineering AG	A035		D. Dialektakis
Fermentor 30 liters (20 liters working capacity)	Bioengineering AG	A035		D. Dialektakis
pH Meter	Janke & Kunkel	A035		D. Dialektakis
Ultra Low Temperature Freezer Jouan	Thermo Scientific	A034		D. Dialektakis
2 Incubators	Memmert	A034		D. Dialektakis
Sonicator	Branson Sonifier	A034		C. Giata C. Vamvakia
CEPAR Laboratory Centrifuge GLE continuous flow centrifuge	Carl Padberg Zentrifugenbau GmbH	A034		D. Dialektakis
French Press Cell Press	SLM AMINCO	A031		C. Giata C. Vamvakia
Controled environment Incubator Shaker	New Brunswick Scientific	A031		D. Dialektakis

Services
Services that we can provide include:
Growth of microbial cultures from 20 ml to 50 liters with typically short lead times (1 to 3 weeks from the placement of orders).
Shipment of cell paste. Cells can be shipped frozen all together or in small aliquots allowing you to use the material as you need it.
Technical advice for production of bacterial cells (including thermophiles, psychrophiles) or fungi cells and their products.
Frozen stocks of bacterial paste.
Preparation of Culture Media.
Maintenance of bacterial strains or other microorganisms.
Harvesting of cultures.

Pricing
Upon Request

Personnel

Contact person

Links
MINOTECH
ATCC
TUV HELLAS