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EcoLab RESEARCH EQUIPMENT
The lab is well equipped for molecular biology, protein purification/analysis and protein biophysics. Main pieces of equipment include:
-3 complete FPLC systems and 3 low pressure systems equipped with UVM/UV2 monitors, fraction collectors and various chromatography media, prepacked columns etc. (Amersham Pharmacia)
-1 Model 491 PrepCell  (BioRad) preparative electrophoresis gel system
(MiniPrepCellInstructionManual-Starter Kit-AssemblyGuide-CoolingPathway-Brochure-TechNoteSummaries)
-1 HPLC system (LC10A-VP) equipped with a SPD-M10AVP photodiode array detector and a Peltier thermostated column chamber (Shimadzu). The system is connected to a PC running Class-VP5 data acquisition and a Size Exclusion Chromatography analysis module.
-1 two dimensional electrophoresis system (IPGphor; AmershamPharmacia),
-6 BioRad Miniprotean gel electrophoresis systems with western blotting attachments and multicasting chambers
-1 BioRad semi-dry and 2 wet western blotting apparati
-1 Cary Eclipse Fluorimeter  (Varian) equiped with a Peltier temperature-controlled four position cuvette holder, a microplate accessory, a SFA-20 stop-flow kinetics accessory (Hi-Tec) and a PC runing the Varian Bio package
-1 DNA sequencing system (AmershamPharmacia)
-2 Biofuge Pico and 2 Biofuge Fresco Eppendorf-type centrifuges (Heraeus-Kendro) and 1 Mikro22R-Hettich
-1 table top high speed Biofuge Stratos centrifuge (Heraeus-Kendro)
-1 Multifuge 3S-R 3Lt low spin refrigerated centrifuge (Heraeus-Kendro)
-1 DirectQ nanopure water unit (Millipore)
-1 Innova refrigerated shaking incubator (New Brunswick Scientific)
-1 TC-412 PCR machine (Techne)
-2 controlled-temperature plate incubators
-1 Chromatography cold-box
-1 Dawn EOS 18-angle laser light scattering detector for HPLC and FPLC (Wyatt instruments)
-1 QELS, dynamic light scattering accesory for the DAWN-EOS (Wyatt instruments)
-1 RID-10A Refractive index detector (Shimadzu)
-1 VP-ITC Isothermal Titration Calorimeter (Microcal)
-1 CO2 incubator for studies of EPEC mediated infection of euaryotic cells
-1 96 well plate reader with a monochromator (Biochrom Asys)
EcoLab COMPUTING
We use Apple Macs for computing and PCs for data collection and specialized applications. All computers are connected through a 1Gbit Ethernet network to the EcoLab server (WinXP-Server on an HP box) and to the IMBB server (MacOS X10.2). We currently use:
1 iMac 27' Intel i7 2.8Ghz (MacOSX-Snow Leopard)
1 PowerMac G4/933 (MacOS X-Tiger) with a 22' Apple Cinema Display
1 MacBookPro 2.66GHz (MacOSX-Snow Leopard)
1 iBook G3/600 combo and 1 iBook G4/1.2 (MacOS X-Tiger)
1 iMac 2.16 GHz Core2Duo/20'' white (MacOS X-Tiger and WinXP thru Parallels 2)
1 iMac 2.16 GHz Core2Duo/20'' silver (MacOS X-Leopard and WinXP thru Parallels 3)
1 iMac 2.4 GHz Core2Duo/20'' silver (MacOS X-Leopard and WinXP thru Parallels 3 and WinXP thru Bootcamp)
1 eMac 1.4 GHz PPC (MacOS X-Tiger)
3 fruity iMacs (MacOS9.2 and MacOS X)
1 DELL Optiplex 760 SF (Intel Core 2 Duo E7300 (2.66GHz,1066MHz,3MB) with a 19" display
1 HP2200 LaserJet printer
1 HP1505 LaserJet printer
1 Apple LaserWriter360 printer (decommissioned)
1 Agfa 1212 Scanner
1 HP6127 Deskjet printer
4 PCs hooked on equipment
1 DELL PC used for bioinfromatics
1 Back-up Server PC equipped with a 320Gb DAT
We run a great variety of general productivity (e.g. Safari, Canvas, iCal, iSync, Keynote, FilemakerPro, Contribute, Office, Parallels) and specialized software (e.g. Enzpack, GraphPadPrism, EndNote, NIH Image, SwissPDBviewer, RasMol, Origin, VectorNTI, MatLab, Astra, Aida etc.)
IMBB and UoC core facilities
We also make use of additional equipment from the IMBB and UoC core facilities such as:
-The proteomics facility (ProFI)
- Surface Plasmon Resonance biosensor (IBIS II  ) with thermostatable waterbath (Lauda) and automatic sample loader
- Storm 840 Phosphorimager (Molecular Dynamics-AmershamPharmacia) (Manual)
- 3 Preparative Ultracentrifuges (Kendro)
- High speed refrigerated centrifuges (5 SorvalKendro and 1 Beckman)
- microplate reader (Asys Hitech)
-5 PCR machines
-4 UV-Vis spectrophotomers (Hitachi and PerkinElmer)
-1 confocal microsope (Zeiss Axioscope 2) using the Radiance 2100 (Biorad) scanning system and -Lasersharp 2000 software.
-1 personal ultracentrifuge (Beckman TLX Optima), equipped with a full array of rotors (TLA-120.2, TLA-100 and TLS- 55 swing out)
-Fermentation equipment (50Lt and 30Lt capacities; Bioengineering AG)
-1 Process-scale protein purification system (Biopilot, AmershamPharmacia)
-DNA sequencing/synthesis (either through Microchemistry or through the IMBB Genome Lab)
-2 Phast mini electrophoresis systems (AmershamPharmacia)
-Protein crystallography
-Chemiluminescence/Fluorescence CCD system (LAS3000; FujiFilm)
-Circular dichroism spectropolarimeter (Model J810; Jasco)
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