The following infrastructure necessary for the research performed in Loukeris lab is provided by IMBB: Animal Cell Culture, Insect Cell Culture , Cell Biology Facility, Fermentation and Protein purification facility, Insectarium – equipped with injection systems (for embryo as well as for adult stage injections), controlled temperature chambers, dissecting microscopes, light microscopes, fluorescence microscopes and stereoscopes. Insectarium has been expanded in order to accomodate the increasing needs for mosquitoes and a separated laboratory has been established, properly equiped to perform safely mosquito infections with P. berghei parasites (P. berghei laboratory). In addition, due to the generous donation of Bodosakis Foundation a separate lab was established in order to perform parasite transgenesis (Transgenic parasite facility).

Apart from parasite transgenesis additional special techiques established in the lab are the following: Artificial Membrane Feeding of mosquitoes that allows the feeding of mosquitoes with artificial blood meal in which peptides with putative antiparasitic function are included, confocal microscopy of mosquito midgut invasion by plasmodium parasites that allows to unravel cellular aspects of midgut invasion and reveal putative differences between mutant and wild type parasites, mosquito cell line- manipulations/inducible expression systems that consist a helpful parallel system to study mosquito genes as well as to validate antiparasitic molecules, functional analysis of genes in anophelines using RNAi methodology that is a widely used methodology to study mosquito gene function.

Currently Loukeris laboratory is implicated with proteomics work, in order to characterize globally transgenic parasite strains and map deviations of mutant parasite total protein content from the normal protein “cargo”. The lab is equipped with a two-dimensional gel electrophoresis system, while in addition, is one of the five founder IMBB laboratories that were lately awarded a Reg ional pot ential (REGPOT) fund from European Community in order to establish a Proteomics facility at IMBB (PROFI laboratory).