Multicellularity is based on cell communication for the correct allocation of cell fates and the maintenance of tissue homeostasis. We study cellular behaviour in the context of developing animal tissues, trying to understand how external signals interface with cell-intrinsic processes, primarily transcription. Our main areas of focus are: (a) the transcriptional regulation of neural development and its interface with Notch signalling and (b) the mechanism of Notch activation by its ligands. Our main model system is the fruitfly Drosophila melanogaster, which enables us to use the most sophisticated molecular genetic technologies available today.
Delidakis C, Monastirioti M, Magadi SS. (2014) E(spl): genetic, developmental, and evolutionary aspects of a group of invertebrate Hes proteins with close ties to Notch signaling. Curr Top Dev Biol. 110:217-62.
Kux K, Kiparaki M, Delidakis C. (2013) The two Tribolium E(spl) genes show evolutionarily conserved expression and function during embryonic neurogenesis. Mech. Dev. 130:207-225.
Monastirioti M, Giagtzoglou N, Koumbanakis KA, Zacharioudaki E, Deligiannaki M, Wech I, Almeida M, Preiss A, Bray S, Delidakis C. (2010) Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis. Development 137:191-201.
Pitsouli C, Delidakis C. (2005) The interplay between DSL proteins and ubiquitin ligases in Notch signaling. Development 132:4041-4050.
Giagtzoglou N, Koumpanakis KA, Fullard JF, Zarifi I, Delidakis C. (2005) Role of the Sc C-terminus in transcriptional activation and E(spl) repressor recruitment. J. Biol. Chem. 280:1299-305.
Cooper M.T.D., Tyler D., Furriols M., Chalkiadaki A., Delidakis C. and Bray S. 2000. Spatially restricted factors co-operate with Notch in the regulation of Enhancer of split genes. Dev. Biol. 221, 390-403.
Nagel A.C., Apidianakis Y., Wech I., Maier D., Delidakis C. and Preiss A. 2000. Neural hyperplasia induced by RNA interference with m4/ma gene activity. Mech. Dev. 98, 19-28.
Apidianakis, Y., Grbavec, D., Stifani, S. and Delidakis, C. 2001. Groucho mediates a Ci-independent mechanism of hedgehog repression in the anterior wing pouch. Development 128, 4361-4370.
Boutla, A., Delidakis, C., Livadaras I., Tsagris, M. and Tabler, M. 2001. Short 5’-phosphorylated double-stranded RNAs induce RNA interference in Drosophila. Curr. Biol. 11, 1776-1780.
Pavlopoulos, E., Pitsouli, C., Klueg, K., Muskavitch, M.A.T., Moschonas, N.K. and Delidakis, C. 2001. neuralized encodes a peripheral membrane protein involved in Delta signalling and endocytosis. Dev. Cell 1, 807-816
Pavlopoulos, E., Kokkinaki, M., Koutelou, E., Mitsiadis, T.A., Prinos, P., Delidakis, C., Kilpatrick, M.W., Tsipouras, P., Moschonas, N.K. 2002. Cloning, chromosomal organization and expression analysis of Neurl(1), the mouse homolog of Drosophila melanogaster neuralized gene. Biochim Biophys Acta 1574, 375-82.
Parkhurst, S.M. and Delidakis, C. 2002. Fly Developments: an introspective. (review) Bioessays 25, 87-89.
Giagtzoglou, N., Alifragis, P., Koumbanakis, K.A. and Delidakis, C. 2003. Two modes of recruitment of E(spl) repressors onto target genes. Development 130, 259-270.
Boutla, A., Delidakis, C. and Tabler, M. 2003. Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes. Nucleic Acids Res, 31, 4973-4980.
Boutla, A., Delidakis, C., Livadaras, I., and Tabler, M. 2003. Variations of the 3' protruding ends in synthetic short interfering RNA (siRNA) tested by microinjection in Drosophila embryos. Oligonucleotides, 13, 295-301.
Giagtzoglou, N., Koumpanakis, K.A., Fullard, J.F., Zarifi, I. and Delidakis, C. 2005. Role of the Sc C-terminus in transcriptional activation and E(spl) repressor recruitment. J. Biol. Chem. 280, 1299-305
Pitsouli, C. and Delidakis, C. 2005. The interplay between DSL proteins and ubiquitin ligases in Notch signaling. Development 132, 4041-4050
Acar, M., Jafar-Nejad, H., Giagtzoglou, N., Yallampalli, S., David, G., He, Y., Delidakis, C. and Bellen, H.J. 2006. Senseless physically interacts with proneural proteins and functions as a transcriptional co-activator. Development 133, 1979-89
Glittenberg, ? ., Pitsouli, C., Garvey, C., Delidakis, C. and Bray, S. 2006. Role of conserved intracellular motifs in Serrate signalling, cis-inhibition and endocytosis. EMBO J . 25, 4697–4706
Bejarano, F., Perez, L., Apidianakis, Y., Delidakis, C. and Milan , M. 2007. Hedgehog restricts its expression domain in the Drosophila wing. EMBO Reports 8 , 778-783
Monastirioti, M., Giagtzoglou, N,. Koumbanakis, K.A., Zacharioudaki, E., Deligiannaki, M., Wech, I. , Almeida, M., Preiss, A., Bray, S. and Delidakis, C. 2010. Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis. Development 137, 191-201
Daskalaki, A., Shalaby, N.A., Kux, K., Tsoumpekos, G., Tsibidis, G.D., Muskavitch, M.A.T. and Delidakis C. 2011. Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized. . J. Cell Biol. 195, 1017-1031
Zacharioudaki, E., Magadi, S. S. and Delidakis, C. 2012. bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation. Development 139, 1258-69
Zarifi I, Kiparaki M, Koumbanakis KA, Giagtzoglou N, Zacharioudaki E, Alexiadis A, Livadaras I, Delidakis C. 2012. Essential roles of Da transactivation domains in neurogenesis and in E(spl)-mediated repression. Mol Cell Biol. 32, 4534-48
Kux, K, Kiparaki, M and Delidakis, C. 2013. The two Tribolium E(spl) genes show evolutionarily conserved expression and function during embryonic neurogenesis. Mech. Dev. 130, 207-225
Delidakis, C. 2014. Tempering temperature changes for robust development. Cell 157, 1010-12
Delidakis, C, Monastirioti, M and Magadi, SS. 2014. E(spl): Genetic, developmental and evolutionary aspects of a group of invertebrate Hes proteins with close ties to Notch signaling. In:Reshma Taneja, editor, Current Topics in Developmental Biology, Vol. 110, Burlington: Academic Press, pp. 217-262
Kiparaki, M., Zarifi, I. and Delidakis, C. 2015. bHLH proteins involved in Drosophila neurogenesis are mutually regulated at the level of stability. Nucl. Acids Res., in press
Zacharioudaki E, Housden BE, Garinis G, Stojnic R, Delidakis C, Bray SJ. 2016. Genes implicated in stem cell identity and temporal programme are directly targeted by Notch in neuroblast tumours. Development. 143(2):219-31