imbb logo for mobile
 

    Publications

    2022
    Krishnamurthy, S., Sardis, M.F., Eleftheriadis, N., Chatzi, K., Smit, J.H., Karathanou, K., Gouridis, G., Portaliou, A., Bondar, A.N., Karamanou S., Economou, A. Preproteins couple the intrinsic dynamics of SecA to its ATPase cycle to translocate via a catch and release mechanism
    (submitted, biorxiv, doi: 10.1101/2021.08.31.458331)

    Muthahari, Y., Tsirigotaki, A., Krishnamurthy, S., Hertadi, R., Pozidis, C., and Gouridis, G. The evolution of allosteric networks revealed by an integrative analysis on an ancestral bilobed protein scaffold
    (submitted)

    Vougioukalaki, M., Xu, R., Konstantinidou, M., Eleftheriadis, N., Dömling, A., and Gouridis, G. Global Ras structural analysis and its direct pharmacological targeting
    (submitted)

    2021
    Gouridis, G., Muthahari, Y., de Boer, M., Griffith, D.A., Tsirigotaki, A., Tassis, K., Zijlstra, N., Xu, R., Eleftheriadis, N., Sugijo, Y., Zacharias, M., Dömling, A., Karamanou, S., Charalambos, P., Economou, A., and Cordes T. Structural dynamics in the evolution of a bilobed protein scaffold
    PNAS 118(49):e2026165118

    Ploetz, E., Schuurman-Wolters, G.K., Zijlstra, N., Jager, A.W., Griffith, D.A., Guskov, A., Gouridis, G., Poolman, B., and Cordes, T. (2021). Structural and biophysical characterization of the tandem substrate binding domains of the ABC importer GlnPQ
    Open biology 11: 200406

    Krishnamurthy, S., Eleftheriadis, N., Karathanou, K., Smit, J.H., Portaliou, A., Chatzi, K., Sardis, M.F., Karamanou S., Bondar, A.N., Gouridis, G., and Economou, A. (2021). A nexus of intrinsic dynamics underlies translocase priming
    Structure 29:846-858

    Tassis, K., Vietrov, R., de Koning, M., de Boer, M., Gouridis, G., and Cordes, T. (2021). Single-molecule studies on conformational states and dynamics in the ABC importer OpuA.
    FEBS Lett 595: 717-734

    2020
    Zernia, S., van der Helde, N., Galenkamp, N.S., Gouridis, G., and Maglia, G. Interpretation of the current blockades of proteins inside nanopores for real-time metabolome analysis
    ACS Nano 14: 2296-2307

    2019
    de Boer, M., Gouridis, G., Muthahari, Y., and Cordes T. Single-Molecule observation of ligand-binding and protein conformation in FeuA
    Biophysical journal 119: 1642-1654

    Gouridis, G, Hetzert, B., Kiosze-Becker, K, de Boer, M., Heinemann, H., Nürenberg-Goloub, E., Cordes, T., and Tampe, R. ABCE1 controls ribosome recycling by an asymmetric dynamic conformational equilibrium
    Cell reports 28:723-734

    de Boer, M., Gouridis, G., Vietrov, R.,  Husada, F., Begg, S.L., Schuurman-Wolters, G.K., Husada, F., Eleftheriadis N., Poolman, B., McDevitt, C., and Cordes T. Conformational and dynamics plasticity in substrate-binding proteins underlies selective transport in ABC importers
    eLife 8:pii: 44652

    2018
    Hellenkamp, B., Schmid, S., (12 authors)., Gouridis, G., (40 authors)., and Hugel, T. Precision and accuracy of single-molecule FRET measurements – a worldwide benchmark study
    Nature methods 15: 669-676

    2017
    Sardis, M.F., Tsirigotaki, A., Chatzi, E.K., Portaliou, G.A., Gouridis, G., Karamanou, S., and Economou, A. Preprotein conformational dynamics drive bivalent translocase docking and secretion
    Structure 25: 1056-67
    See comment in faculty of 1000 by Karin Roemisch

    2016
    van der Velde, J.H., Oelerich, J., Huang, J., Smit, J.H., Aminian Jazi, A., Galiani, S., Kolmakov, K., Gouridis, G., Eggeling, C., Herrmann, A., Roelfes, G. and Cordes, T. A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.
    Nature Communications 7: 10144

    2015
    Husada, F., Gouridis, G., Vietrov, R., Schuurman-Wolters, G.K., Ploetz, E., de Boer, M., Poolman, B. and Cordes, T. Watching conformational dynamics of ABC transporters with single-molecule tools.
    Biochemical Society Transactions 43: 1041-7

    Gouridis, G., Schuurman-Wolters, G.K., Ploetz, E., Husada, F., Vietrov, R., de Boer, M., Cordes, T. and Poolman, B. Conformational dynamics in substrate-binding domains influences transport in the ABC importer GlnPQ.
    Nature Structural & Molecular Biology 22: 57-64

    2013
    Gouridis, G., Karamanou, S., Sardis, M.F., Schärer, M.A., Capitani, G. and Economou, A. Quaternary dynamics of the SecA motor drive translocase catalysis.
    Molecular Cell. 52: 655-66

    2010
    Gouridis, G., Karamanou, S., Koukaki, M. and Economou, A. In vitro assays to analyze translocation of the model secretory preprotein alkaline phosphatase.
    Methods in Molecular Biology 619, 157-72

    2009
    Gouridis, G., Karamanou, S., Gelis, I., Kalodimos, C.G., and Economou, A. (2009) Signal peptides are allosteric activators of the protein translocase.
    Νature 462, 363-36

    2007
    Gelis, I., Bonvin, A.M.J.J., Keramisanou, D., Koukaki, M., Gouridis, G., Karamanou, S., Economou, A. and Kalodimos, C.G. (2007) Structural Basis for Signal-Sequence Recognition by the Translocase Motor SecA as Determined by NMR
    Cell 131, 756-769
    See comment from Faculty of 1000; see presentation by Thireos et al., 2008

    Karamanou, S., Gouridis, G., Papanikou, E., Sianidis, G., Gelis, I., Keramisanou, D., Vrontou, E., Kalodimos, C.G., and Economou, A. (2007) Preprotein-controlled catalysis in the helicase motor of SecA.
    EMBO Journal 26, 2904-2911
    See comment from Faculty of 1000; see article in LabTimes

    2005
    Karamanou, S., Sianidis, G., Gouridis, G., Pozidis C., Papanikolau, Y., Papanikou, E., and Economou, A. (2005) E. coli SecA truncated at its termini is functional and dimeric.
    FEBS Letters 579, 1267-1271