The laboratory is located at the IMBB building (Room A205), and is well equipped for routine molecular biology and biochemistry experimental work. Basic equipment includes various incubators, centrifuges, electrophoresis and blotting apparatuses, a spin-vacuum concentrator, a gradient thermal cycler, a UV-VIS spectrophotometer and a spectrofluorometer for HTS and kinetic measurements.
We share with other groups cold-rooms, large incubators, the tissue culture facility, imaging and scintillation analysis equipment. Fast DNA sequencing, oligonucleotide synthesis and fermentation services are provided by the DNA sequencing, Microchemistry and Fermentation & Purification facilities of IMBB.
Microarray equipment, in particular, includes a Packard SpotArray 24 spotter, a four-laser GSI Lumonics ScanArray 5000, a Tecan HS4800 microfluidic hybridization station, an UltraLum CEX-1500 UV CrossLinker and a Beckman Biomek 2000 robotic system for high-throughput liquid handling procedures.
The massive amounts of data generated by microarray analysis are managed with the BioArray Software Environment (BASE) that run on a dedicated serner.
Finally, the library of FORTH and the nearby library of the University of Crete provide access to most scientific journal by either print or on-line subscription. The helpdesk of the Industrial Property Organization (Greek Patent Office) in FORTH provides free on-line searches and access to world patent libraries.
Packard SpotArray 24
The SpotArray™ 24 is a compact and lightweight microarray printing system. Microarrays are printed with speed and precision using 1-32 Telechem Stealth pins. After printing, the pins are washed with a powerful pressure-jet pin washer and then vacuum-dried, eliminating measurable carryover. After protocol parameters are entered, several instrument sensors verify these parameters and instrument settings, ensuring error-free operation.
The SpotArray 24 is equipped with environmental and humidity control to ensure accurate and consistent printing.
Scanarray 5000 Microarray Scanner
The microarray scanner (ScanArray 5000, PerkinElmer Life Sciences) is a confocal microscope scanning device used for reading out microarrays of standard format (75mmx25mmx1 mm). For excitation of fluorescence 4 lasers can be used plus one external. The emitted fluorescence light is detected by a sensitive photomultiplier tube (PMT).
4 Laser system:
- 488 nm Ar ion laser
- 543 nm HeNe laser
- 594 nm HeNe laser
- 633 nm HeNe laser
12 filters allow the use of a variety of different dyes
max resolution: 5 um, max laser power: ca. 10-20 mW
Detection system: Photomultiplier (PMT)
Sensitivity: 0.1 Fluorophor/ um
Image format: 16 bit tif (max. 65535 grey scale values)
Tecan HS4800 Hybridization Station
The Tecan HS4800 is used for automated hybridization of standard format microarray slides. The advantage is the full automation, i.e. the HS4800 is performing all protocol steps in a highly accurate and reproducible manner. It consists of 12 heating blocks with hybridization chambers on top. The HS4800 controls the temperature and the processing times (hybridization, washing). The hybridization solution (100 ul) is applied by a disposable pipette tip. Due to a highly efficient agitation of the sample within the chamber, the hybridization is faster and more even than under a cover slip. The HS4800 also dries the slides.
UltraLum CEX-1500 UV CrossLinker
- Microprocessor Controlled
- Fully Programmable
- 254, 300 and 365nm Units Available
- 6 Lamp Units
- Stainless Steel Slide Out Tray
- Instant Push Button Intensity Readout
- All Electronic Design Eliminates Lamp Flicker During Startup allowing for Consistent Exposure Everytime!
- Optical Feedback to Monitor Lamp Output
- Special Push Button Intensity Switch
- Easy Opening Door with Lamp Cuttoff Circuit to Eliminate Possible UV Exposure
- UV Blocking Window for Viewing Samples During Exposure
- Stainless Steel Tray for Easy Cleaning and Loading of Sample
The Biomek 2000 is a small-volume pipetting workstation capable of accessing up to 12 microtitre plates at one time. Tools included with the workstation include:
- two 8-channel multiple pipettors (2-20 µl and 20-200 µl),
- a single-channel pipettor that can sense liquid levels (100-1000 µl), and
- a wash unit capable of delivering and aspirating larger volumes (>200 µl)
This combination of tools is capable of addressing labware with 24-well, 96-well, or 384-well formats as well as tubes of various shapes and arrangements.