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Inga Siden-Κιάμος

Inga Siden-Κιάμος

Κύρια Ερευνήτρια
Ηλ. Ταχυδρομείο
Αυτή η διεύθυνση ηλεκτρονικού ταχυδρομείου προστατεύεται από τους αυτοματισμούς αποστολέων ανεπιθύμητων μηνυμάτων. Χρειάζεται να ενεργοποιήσετε τη JavaScript για να μπορέσετε να τη δείτε.
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Ιστοσελίδα Εργαστηρίου
 

Η ελονοσία είναι μια ασθένεια  με  τεράστιες αρνητικές επιπτώσεις στην παγκόσμια υγεία και την οικονομική ανάπτυξη, με περίπου μισό εκατομμύριο θανάτους ετησίως. Η ασθένεια είναι διαδεδομένη κυρίως στις λιγότερο αναπτυγμένες χώρες της Αφρικής και της νοτιοανατολικής Ασίας. Η ελονοσία αντιμετωπίζεται με την χρήση φαρμάκων που στοχεύουν τα παράσιτα του ασθενούς και με εντομοκτόνα για την καταπολέμηση των κουνουπιών-φορέων του παρασίτου. Ωστόσο η εμφάνιση ανθεκτικότητας των παρασίτων στα φάρμακα και των κουνουπιών στα εντομοκτόνα απειλούν αυτές τις στρατηγικές καταπολέμησης. Δεν υπάρχει διαθέσιμο εμβόλιο.

Η ελονοσία οφείλεται σε μονοκύτταρα πρωτόζωα του γένος Plasmodium  που μεταδίδονται από κουνούπια  του γένος Anopheles. Για την εξάλειψη της ασθένειας σε τοπικό ή παγκόσμιο επίπεδο είναι απαραίτητη η χρήση μεθόδων που θα εμποδίζουν την μετάδοση του παρασίτου μέσω των κουνουπιών. Όμως το γεγονός ότι, οι γνώσεις για τα στάδια του παρασίτου στο κουνούπι είναι περιορισμένες, δυσκολεύει την ανάπτυξη τέτοιων μεθόδων.  

Η έρευνα στο εργαστήριό μας εστιάζει στην κατανόηση σημαντικών διεργασιών που συμβαίνουν στα αναπτυξιακά στάδια του παρασίτου στο κουνούπι, χρησιμοποιώντας συνδυαστικά τεχνικές μοριακής και κυτταρικής βιολογίας και αντίστροφής γενετικής. Τις  δικές μας γνώσεις και ικανότητες συμπληρώνουν συνεργαζόμενα εργαστήρια ανά του κόσμο. 

 

Lopez AJ, Andreadaki M, Vahokoski J, Deligianni E, Calder LJ, Camerini S, Freitag A, Bergmann U, Rosenthal PB, Sidén-Kiamos I, Kursula I. (2023) Structure and function of Plasmodium actin II in the parasite mosquito stages. PLoS Pathog. 19:e1011174.

Deligianni E, Kiamos IS. (2021) Gene editing in Plasmodium berghei made easy: Development of a CRISPR/Cas9 protocol using linear donor template and ribozymes for sgRNA generation. Mol Biochem Parasitol. 246:111415.

Siden-Kiamos I, Spanos L, Currà C. (2020) A method for purification of Plasmodium oocysts from mosquito midguts. Sci Rep. 10:7262.

Andreadaki M, Hanssen E, Deligianni E, Claudet C, Wengelnik K, Mollard V, McFadden GI, Abkarian M, Braun-Breton C, Siden-Kiamos I. (2018) Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood. Cell. Sci. Rep. 8:3543.

Currà C, Gessmann R, Pace T, Picci L, Peruzzi G, Varamogianni-Mamatsi V, Spanos L, Garcia CR, Spaccapelo R, Ponzi M, Siden-Kiamos I (2016) Release of Plasmodium sporozoites requires proteins with histone-fold dimerization domains. Nat Commun. 7:13846.

2023

Lopez AJ, Andreadaki M, Vahokoski J, Deligianni E, Calder LJ, Camerini S, Freitag A, Bergmann U, Rosenthal PB, Sidén-Kiamos I, Kursula I. (2023) Structure and function of Plasmodium actin II in the parasite mosquito stages. PLoS Pathogens PLoS Pathog. 2023 Mar 6;19(3):e1011174. doi: 10.1371/journal.ppat.1011174.
Pubmed

2022

Siden-Kiamos I, Koidou V, Livadaras I, Skoufa E, Papadogiorgaki S, Papadakis S, Chalepakis G, Ioannidis P, Vontas J. (2022). Dynamic interactions between the symbiont Candidatus Erwinia dacicola and its olive fruit fly host Bactrocera oleae. Insect Biochem Mol Biol. 2022 Jul;146:103793. doi: 10.1016/j.ibmb.2022.103793. Epub 2022 May 23. PMID: 35618174.
Pubmed

Hamelmann NM, Paats JD, Avalos-Padilla Y, Lantero E, Spanos L, Siden-Kiamos I, Fernàndez-Busquets X, Paulusse JMJ. (2022) Single-Chain Polymer Nanoparticles Targeting the Ookinete Stage of Malaria Parasites. ACS Infectious Diseases DOI: 10.1021/acsinfecdis.2c00336.
Pubmed

2021

Deligianni E, Kiamos IS. (2021) Gene editing in Plasmodium berghei made easy: Development of a CRISPR/Cas9 protocol using linear donor template and ribozymes for sgRNA generation. Mol Biochem Parasitol. 2021 Sep 16;246:111415. doi: 10.1016/j.molbiopara.2021.111415.
Pubmed

Livadaras I, Koidou V, Pitsili E, Moustaka J, Vontas J, Siden-Kiamos I. Stably inherited transfer of the bacterial symbiont Candidatus Erwinia dacicola from wild olive fruit flies Bactrocera oleae to a laboratory strain. Bull Entomol Res. 2021 Jun;111(3):379-384. doi: 10.1017/S0007485321000031.
Pubmed

2020

Grasso F, Mochi S, Fratini F, Olivieri A, Currà C, Siden-Kiamos I, Deligianni E, Birago C, Picci L, Pizzi E, Pace T, Ponzi M. (2020) A comprehensive gender-related secretome of Plasmodium berghei sexual stages. Mol Cell Proteomics. 2020 Dec;19(12):1986-1997. doi: 10.1074/mcp.RA120.002212. Epub 2020 Sep 3. PMID: 32883804.
Pubmed

Andreadaki M, Pace T, Grasso F, Siden-Kiamos I, Mochi S, Picci L, Bertuccini L, Ponzi M, Currà C. (2020) Plasmodium berghei Gamete Egress Protein is required for fertility of both genders Microbiology Open. Microbiologyopen. 2020 Jul;9(7):e1038. doi: 10.1002/mbo3.1038. Epub 2020 Apr 30. PMID: 32352241.
Pubmed

Biosca A, Bouzón-Arnáiz I, Spanos L, Siden-Kiamos I, Iglesias V, Ventura S, Fernàndez-Busquets X. (2020) Detection of massive Protein Aggregation in Live Plasmodium Parasites. Antimicrobial Agents and Chemotherapy, 2020 May 21;64(6):e02135-19. doi: 10.1128/AAC.02135-19. Print 2020 May 21. PMID: 32284383.
Pubmed

Siden-Kiamos I, Spanos L, Currà C. (2020) A method for purification of Plasmodium oocysts from mosquito midguts. Sci Rep. 2020 Apr 29;10(1):7262. doi: 10.1038/s41598-020-64121-1. PMID: 32350329.
Pubmed

Siden-Kiamos I, Goosmann C, Buscaglia CA, Brinkmann V, Matuschewski K, Montagna GN. (2019) Polarization of MTIP is a signature of gliding locomotion in Plasmodium ookinetes and sporozoites. Mol Biochem Parasitol. 2020 Jan;235:111247. doi: 10.1016/j.molbiopara.2019.111247.
Pubmed

2019

Currà C, Kehrer J, Lemgruber L, Silva PAGC, Bertuccini L, Superti F, Pace T, Ponzi M, Frischknecht F, Siden-Kiamos I, Mair GR. (2019) Malaria transmission through the mosquito requires the function of the OMD protein. PLoS One. 2019 Sep 25;14(9):e0222226. doi: 10.1371/journal.pone.0222226. eCollection 2019.
Pubmed

2018

Siden-Kiamos I, Pace T, Klonizakis A, Nardini M, Garcia CRS, Currà C. (2018) Identification of Plasmodium berghei Oocyst Rupture Protein 2 (ORP2) domains involved in sporozoite egress from the oocyst. Int J Parasitol. 2018 Dec;48(14):1127-1136. doi: 10.1016/j.ijpara.2018.09.004.
Pubmed

Martí Coma-Cros E, Biosca A, Marques J, Carol L, Urbán P, Berenguer D, Riera MC, Delves M, Sinden RE, Valle-Delgado JJ, Spanos L, Siden-Kiamos I, Pérez P, Paaijmans K, Rottmann M, Manfredi A, Ferruti P, Ranucci E, Fernàndez-Busquets X. (2018) Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs. Pharmaceutics. 2018 Nov 10;10(4). pii: E225. doi: 10.3390/pharmaceutics10040225.
Pubmed

Deligianni E, Silmon de Monerri NC, McMillan PJ, Bertuccini L, Superti F, Manola M, Spanos L, Louis C, Blackman MJ, Tilley L, Siden-Kiamos I. (2018) Essential role of Plasmodium perforin-like protein 4 in ookinete midgut passage. PLoS One. 2018 Aug 13;13(8):e0201651. doi: 10.1371/journal.pone.0201651.
Pubmed

Goulielmaki E, Kaforou S, Venugopal K, Loukeris TG, Siden-Kiamos I, Koussis K. (2018) Distinct effects of HIV protease inhibitors and ERAD inhibitors on zygote to ookinete transition of the malaria parasite. Mol Biochem Parasitol 220, March 2018, Pages 10-14.
Pubmed

Kefi M, Mavridis K, Simões ML, Dimopoulos G, Siden-Kiamos I, Vontas J. (2018) New rapid one-step PCR diagnostic assay for Plasmodium falciparum infective mosquitoes. Sci. Rep. 23;8(1):1462. doi: 10.1038/s41598-018-19780-6.
Pubmed

Deligianni E, Andreadaki M, Koutsouris K, Siden-Kiamos I. (2018) Sequence and functional divergence of gametocyte-specific parasitophorous vacuole membrane proteins in Plasmodium parasites. Mol Biochem Parasitol 10.1016/j.molbiopara.2018.01.002.
Pubmed

Andreadaki M, Hanssen E, Deligianni E, Claudet C, Wengelnik K, Mollard V, McFadden GI, Abkarian M, Braun-Breton C, Siden-Kiamos I. (2018) Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell. Sci. Rep. Feb 23;8(1):3543. doi: 10.1038/s41598-018-21801-3.
Pubmed

2017

Koussis K, Goulielmaki E, Chalari A, Withers-Martinez C, Siden-Kiamos I, Matuschewski K, Loukeris TG. (2017) Targeted Deletion of a Plasmodium Site-2 Protease Impairs Life Cycle Progression in the Mammalian Host. PLoS One. 12(1):e0170260
Pubmed

2016

Currà C, Gessmann R, Pace T, Picci L, Peruzzi G, Varamogianni-Mamatsi V, Spanos L, Garcia CR, Spaccapelo R, Ponzi M, Siden-Kiamos I (2016) Release of Plasmodium sporozoites requires proteins with histone-fold dimerization domains. Nat Commun. 2016 Dec 16;7:13846. doi: 10.1038/ncomms13846.
Pubmed

Andreadaki M, Deligianni E, Nika F, Siden-Kiamos I. (2016) Expression of the Plasmodium berghei actin II gene is controlled by elements in a long genomic region. Parasitol Res. 115(8):3261-5. 
Pubmed

2015

Andreadaki M, Mollenkopf HJ, Nika F, Brady D, Tewari R, Matuschewski K, Siden-Kiamos I. (2015) Global expression profiling reveals shared and distinct transcript signatures in arrested act2(-) and CDPK4(-) Plasmodium berghei gametocytes. Mol Biochem Parasitol.201(2):100-7.
Pubmed

Tampaki Z, Mwakubambanya RS, Goulielmaki E, Kaforou S, Kim K, Waters AP, Carruthers VB, Siden-Kiamos I, Loukeris TG, Koussis K. (2015) Ectopic Expression of a Neospora caninum Kazal Type Inhibitor Triggers Developmental Defects in Toxoplasma and Plasmodium. PLoS One. 2015 Mar 24;10(3):e0121379. doi: 10.1371/journal.pone.0121379
Pubmed

Olivieri A, Bertuccini L, Deligianni E, Franke-Fayard B, Currà C, Siden-Kiamos I, Hanssen E, Grasso F, Superti F, Pace T, Fratini F, Janse CJ, Ponzi M. (2015) Distinct properties of the egress-related osmiophilic bodies in male and female gametocytes of the rodent malaria parasite Plasmodium berghei. Cell Microbiol. 2015 Mar; 17(3):355-68. doi: 10.1111/cmi.12370
Pubmed

Hliscs M, Millet C, Dixon MW, Siden-Kiamos I, McMillan P, Tilley L. (2015) Organisation and function of an actin cytoskeleton in Plasmodium falciparum gametocytes. Cell Microbiol. 2015 Feb;17(2):207-25. doi: 10.1111/cmi.12359
Pubmed

2014

Guerreiro A, Deligianni E, Santos J. M, Silva P, Louis C, Pain A, Janse C. J, Franke-Fayard B, Carret C. K, Sidén-Kiamos I, Mair G R. (2014) Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte. Genome Biology 15:493
Pubmed

Goulielmaki E, Sidén-Kiamos I, Loukeris TG. (2014) Functional Characterization of Anopheles Matrix Metalloprotease 1 Reveals Its Agonistic Role during Sporogonic Development of Malaria Parasites. Infect Immun. 2014 Nov;82(11):4865-77. doi: 10.1128/IAI.02080-14
Pubmed

Andreadaki, M., Morgan, R.N., Deligianni, E., Kooij T.W.A., Santos, J.M., Spanos, L., Matuschewski, K., Louis, C., Mair, G.R., and Siden-Kiamos, I. (2014) Genetic crosses and complementation reveal essential functions for the Plasmodium stage-specific actin2 in sporogonic development. Cell. Microbiol. 16(5):751-67. doi: 10.1111/cmi.12274
Pubmed

Vahokoski, J., Bhargav, S.P., Desfosses, A., Andreadaki, M., Kumpula, E.-P., Martinez, S.M., Ignatev, A., Lepper, S., Frischknecht, F., Sidén-Kiamos, I., Sachse, C., and Kursula, I. (2014) Structural Differences Explain Diverse Functions of Plasmodium Actins. PLoS Pathog. Apr 17;10(4):e1004091. doi: 10.1371/journal.ppat.1004091.
Pubmed

2013

Deligianni, E., Morgan, R.N., Bertuccini, L., Wirth, C.C., Silmon de Monerri, N.C., Spanos, L., Blackman, M.J., Louis , C., Pradel, G., and Siden-Kiamos, I. (2013) A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes Cell. Microbiol. 15(8):1438-55, Aug 2013
Pubmed

Marta Ponzi , Inga Sidén-Kiamos, Lucia Bertuccini, Chiara Currà, Hans Kroeze, Grazia Camarda, Tomasino Pace, Blandine Franke-Fayard, Eliane C. Laurentino, Christos Louis, Andrew P. Waters, Chris J. Janse, Pietro Alano. (2013) The ETRAMP family member SEP2 is expressed throughout Plasmodium berghei life cycle and is released during sporozoite gliding motility. PLOS One 8(6):e67238, Jun 2013 Pubmed

2012

Siden-Kiamos, I., Louis, C., Matuschewski, K. (2012). Evidence for filamentous actin in ookinetes of a malarial parasite. Mol. Biochem. Parasitol. 181(2):186-9
Pubmed

2011

Deligianni E, Morgan RN, Bertuccini L, Kooij TW, Laforge A, Nahar C, Poulakakis N, Schüler H, Louis C, Matuschewski K, Siden-Kiamos I. (2011) Critical role for a stage-specific actin in male exflagellation of the malaria parasite. Cell Microbiol. 13:1714-1730
Pubmed

Siden-Kiamos I, Ganter M, Kunze A, Hliscs M, Steinbüchel M, Mendoza J, Sinden RE, Louis C, Matuschewski, K. (2011) Stage-specific Depletion of Myosin A Supports an Essential Role in Motility of Malarial Ookinetes. Cell Microbiol.13:1996-2006
Pubmed

2010

Siden-Kiamos I, Schüler H, Liakopoulos D, Louis C. (2010) Arp1, an actin-related protein, in Plasmodium berghei. Mol Biochem Parasitol. 173(2):88-96
Pubmed

2009

Ponzi M, Sidén-Kiamos I, Bertuccini L, Currà C, Kroeze H, Camarda G, Pace T, Franke-Fayard B, Laurentino EC, Louis C, Waters AP, Janse CJ, Alano P. (2009) Egress of Plasmodium berghei gametes from their host erythrocyte is mediated by the MDV-1/PEG3 protein. Cell Microbiol. 11(8):1272-88
Pubmed