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Δόμνα Καραγωγέως

Δόμνα Καραγωγέως

Ερευνήτρια IMBB (Καθηγήτρια, Πανεπιστήμιο Κρήτης)
Ηλ. Ταχυδρομείο
Αυτή η διεύθυνση ηλεκτρονικού ταχυδρομείου προστατεύεται από τους αυτοματισμούς αποστολέων ανεπιθύμητων μηνυμάτων. Χρειάζεται να ενεργοποιήσετε τη JavaScript για να μπορέσετε να τη δείτε.
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Ιστοσελίδα Εργαστηρίου

Το ενδιαφέρον του εργαστηρίου εστιάζεται στην ανάπτυξη του νευρικού συστήματος και τις αξονογλοιακές αλληλεπιδράσεις κατά την μυελίνωση.

Ανάπτυξη του νευρικού συστήματος: μελέτη της γενετικής, μοριακής, κυτταρικής βάσης της νευρογένεσης, της νευρωνικής μετανάστευσης και της καθοδήγησης νευραξόνων στους στόχους τους. Συγκεκριμένα μελετούμε:
1) το ρόλο της πρωτεΐνης TAG -1/ Contactin -2, μέλους της υπεροικογένειας των ανοσοσφαιρινών, και των πρωτεϊνών με τις οποίες αλληλεπιδρά, στην καθοδήγηση των νευραξόνων και την νευρωνική μετανάστευση
2) το ρόλο των μικρών Rho GTP ασών στην ανάπτυξη των ενδονευρώνων του φλοιού

Αξονογλοιακές αλληλεπιδράσεις στην μυελίνωση: οι εμμύελες ίνες οργανώνονται σε διακριτές περιοχές σαν αποτέλεσμα αξονογλοιακών αλληλεπιδράσεων, όπως ο κόμβος του Ranvier , η παρακομβική και η εγγύς-της-παρακομβικής περιοχής. Μελετούμε τους μηχανισμούς που επιφέρουν την οργάνωση και μοριακή αρχιτεκτονική των εμμύελων ινών έτσι ώστε να επιτυγχάνεται η ταχεία μεταγωγή της νευρικής ώσης. Ιδιαίτερα μελετούμε τον ρόλο της TAG -1/ Contactin -2 και των συμπλόκων της στην οργάνωση της εγγύς-της-παρακομβικής περιοχής και πως η ρύθμιση της έκφρασής της μεταβάλλεται σε ζωϊκά μοντέλα απομυελίνωσης (π.χ. που μιμούνται την Κατά Πλάκας Σκλήρυνση). Παράλληλα μελετούμε μηχανισμούς δημιουργίας των φραγμών διαπερατότητας στη Δροσόφιλα ώστε να κατανοήσουμε περαιτέρω βασικές μοριακές και κυτταρικές πτυχές των περιοχών που είναι ανάλογες των παρακομβικών περιοχών στα θηλαστικά.

Πειραματικές προσεγγίσεις: χρησιμοποιούμε συνδυασμό γενετικών, μοριακών, βιοχημικών και κυτταρικών μεθόδων όπως διαγονιδιακά ζωϊκά μοντέλα, ex - vivo καλλιέργειες ιστών και κυττάρων, συνεστιακή μικροσκοπία, γενετική ανάλυση σε Δροσόφιλα, RNA μικροσυστοιχίες, real time - PCR, και πρωτεϊνική βιοχημεία. Ηλεκτοφυσιολογική και συμπεριφορική ανάλυση διαγονιδιακών μοντέλων μέσω συνεργασιών.

Savvaki M, Theodorakis K, Zoupi L, Stamatakis A, Tivodar S, Kyriacou K, Stylianopoulou F, Karagogeos D. (2010) The Expression of TAG-1 in Glial Cells is Sufficient  for the Formation of the Juxtaparanodal Complex and the Phenotypic  Rescue of Tag-1 Homozygous Mutants in the CNS. J. Neurosci 30: 13943-54.

Ma QH, Futagawa T, Yang WL, Jiang XD, Zeng L, Takeda Y, Xu RX, Bagnard D, Schachner M, Furley AJ, Karagogeos D, Watanabe K, Dawe GS, Xiao ZC. (2008) A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis. Nat Cell Biol. 10: 283-94

Strigini M., Cantera R, Morin X, Bastiani M, Bate M, Karagogeos D. (2006) The IgLON protein Lachesin, is required for the blood-brain barrier in Drosophila. Mol Cell Neurosci 32 :91-101.

Denaxa M, Kyriakopoulou K, Theodorakis K, Trichas G, Vidaki M, Takeda Y, Watanabe K, Karagogeos D. (2005) The adhesion molecule TAG-1 is required for proper migration of the superficial migratory stream in the medulla but not of cortical interneurons. Dev. Biol. 288: 87-99.

Traka M, Goutebroze L, Denisenko N, Bessa M, Nifli F, Havaki S, Iwakura Y, Fukamauchi F, Watanabe K, Girault J.A. Karagogeos D. (2003) TAG-1 associates with Caspr-2 and is essential for the molecular organization of juxtaparanodal regions of myelinated fibers. J Cell Biol 162: 1161-1172.

Recent Publications (since 2000)

Pinatel D, Hivert B, Saint-Martin M, Noraz N, Savvaki M, Karagogeos D, Faivre-Sarrailh C. (2017) The Kv1-associated molecules TAG-1 and Caspr2 are selectively targeted to axon initial segment in hippocampal neurons. J Cell Sci. 2017 May 22. pii: jcs.202267. doi: 10.1242/jcs.202267

Konstantoudaki X, Chalkiadaki K, Tivodar S, Karagogeos D, Sidiropoulou K. (2016) Impaired synaptic plasticity in the prefrontal cortex of mice with developmentally decreased number of interneurons. Neuroscience. 2016 May 13;322:333-45

Bastakis GG, Savvaki M, Stamatakis A, Vidaki M, Karagogeos D. (2015) Tag1 deficiency results in olfactory dysfunction through impaired migration of mitral cells. Development. 2015 Dec 15;142(24):4318-28

Pinatel D, Hivert B, Boucraut J, Saint-Martin M, Rogemond V, Zoupi L, Karagogeos D, Honnorat J, Faivre-Sarrailh C. (2015) Inhibitory axons are targeted in hippocampal cell culture by anti-Caspr2 autoantibodies associated with limbic encephalitis. Front Cell Neurosci. 2015 Jul 9;9:265

Kastriti ME, Sargiannidou I, Kleopa KA, Karagogeos D. (2015) Differential modulation of the juxtaparanodal complex in Multiple Sclerosis. Mol Cell Neurosci. 2015 Jul;67:93-103

Tsikala G, Karagogeos D, Strigini M. (2014) Btk-dependent epithelial cell rearrangements contribute to the invagination of nearby tubular structures in the posterior spiracles of Drosophila.Dev Biol 396(1): 42-56.

Schmidt ER, Brignani S, Adolfs Y, Lemstra S, Demmers J, Vidaki M, Donahoo AL, Lilleväli K, Vasar E, Richards LJ, Karagogeos D, Kolk SM, Pasterkamp RJ. (2014) Subdomain-mediated axon-axon signaling and chemoattraction cooperate to regulate afferent innervation of the lateral habenula. Neuron. 2014 83(2):372-87

Tivodar S, Kalemaki K, Kounoupa Z, Vidaki M, Theodorakis K, Denaxa M, Kessaris N, de Curtis I, Pachnis V, Karagogeos D. (2014) Rac-GTPases Regulate Microtubule Stability and Axon Growth of Cortical GABAergic Interneurons. Cereb Cortex. 2014 Apr 1. [Epub ahead of print]

Zoupi L, Markoullis K, Kleopa KA, Karagogeos D. (2013) Alterations of juxtaparanodal domains in two rodent models of CNS demyelination. Glia. 61(8):1236-49

Katidou M, Tavernarakis N, Karagogeos D. (2013) The contactin RIG-6 mediates neuronal and non-neuronal cell migration in Caenorhabditis elegans. Dev Biol. 373(1):184-95

Vidaki M, Tivodar, S., Doulgeraki K, Tybulewicz, V., Kessaris N, Pachnis, V. and Karagogeos D. (2012) Rac1-Dependent Cell Cycle Exit of MGE Precursors and GABAergic Interneuron Migration to the Cortex. Cerebral Cortex 22(3):680-92

Zoupi L, Savvaki M, Karagogeos D. (2011) Axons and myelinating glia: An intimate contact. IUBMB Life. 63(9):730-5

Wang, W., Karagogeos, D . and Kilpatrick, DL. (2011) The effects of Tag-1 on the maturation of mouse cerebellar granule neurons. Cell Mol Neurobiol. 31(3):351-6

Savvaki, M, Theodorakis, K., Zoupi, L., Stamatakis, A., Tivodar, S., Kyriacou, K., Stylianopoulou, F., and Karagogeos, D. (2010) The Expression of TAG-1 in Glial Cells is Sufficient  for the Formation of the Juxtaparanodal Complex and the Phenotypic  Rescue of Tag-1 Homozygous Mutants in the CNS. J. Neurosci., 30(42):13943-54

Sakurai K, Toyoshima M, Ueda H, Matsubara K, Takeda Y, Karagogeos D, Shimoda Y, Watanabe K. (2009) Contribution of the neural cell recognition molecule NB-3 to synapse formation between parallel fibers and Purkinje cells in mouse. Dev Neurobiol. 69(12):811-2

Derfuss T., Parikh K., Velhin S., Braun M., Mathey E., Krumbholz M., Kümpfel T., Moldenhauer A., Kunz B., Pöllmann W, Tiefenthaller C, Bauer J, Lassmann H, Wekerle , H., Karagogeos D , Hohlfeld R., Linington C., Meinl E. (2009) Contactin-2/Tag-1 directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals PNAS USA 106(20):8302-7

Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D , Schachner M, Kleene R . (2009) Lewis (x) and alpha 2,3 sialyl glycans and their receptors TAG-1, Contactin and L1 mediate CD24-dependent neurite outgrowth J. Neurosci. 29(20):6677-90

Katidou M, Vidaki M, Strigini M, Karagogeos D. (2008) The immunoglobulin superfamily of neuronal cell adhesion molecules: lessons from animal models and correlation with human disease Biotechnol J . 3(12):1564-80

Savvaki M, Panagiotaropoulos T, Stamatakis A, Sargiannidou I, Karatzioula P, Watanabe K, Stylianopoulou F, Karagogeos D, Kleopa KA. (2008) Impairment of learning and memory in TAG-1 deficient mice associated with shorter CNS internodes and disrupted juxtaparanodes. Mol Cell Neurosci . 39(3):478-90

Chatzopoulou E, Miguez A, Savvaki M, Levasseur G, Muzerelle A, Muriel MP, Goureau O, Watanabe K, Goutebroze L, Gaspar P, Zalc B, Karagogeos D, Thomas JL. (2008) Structural requirement of TAG-1 for retinal ganglion cell axons and myelin in the mouse optic nerve. J Neurosci . 23;28(30):7624-36

Ma QH, Futagawa T, Yang WL, Jiang XD, Zeng L, Takeda Y, Xu RX, Bagnard D, Schachner M, Furley AJ, Karagogeos D, Watanabe K, Dawe GS, Xiao ZC. (2008) A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis. Nat Cell Biol . 10(3):283-94. Erratum in: Nat Cell Biol. 2008, 10(4):497

McLaughlin D, Vidaki M, and Karagogeos D. (2008) Localization of CRMP5 mRNA by in situ hybridisation during development of the mouse forebrain. Neurosci Lett . 432(2):117-20

Tzimourakas, A., Giasemi, S., Mouratidou, M., and Karagogeos, D. (2007) Structure-function analysis of protein complexes involved in the molecular architecture of juxtaparanodal regions of myelinated fibers Biotechnol. J 2(5):577-83

Strigini, M., Cantera, R., Morin, X., Bastiani, M., Bate, M., and Karagogeos, D. (2006) The IgLON protein Lachesin, is required for the blood-brain barrier in Drosophila Mol Cell Neurosci. 32(1-2):91-101.

McLaughlin, D., Vidaki, M., Renieri, E. and Karagogeos, D. (2006) Expression pattern of the maternally imprinted gene Gt12 in the forebrain during embryonic development and adulthood Mech Dev GEP 6(4):394-9

Denaxa, M., Kyriakopoulou, K., Theodorakis, K., Trichas, G., Vidaki, M., Takeda, Y., Watanabe, K., and Karagogeos, D. (2005) The adhesion molecule TAG-1 is required for proper migration of the superficial migratory stream in the medulla but not of cortical interneurons Dev. Biol. 288(1):87-99

Ekonomou,A., Kazanis,I., Malas,S., Wood,H., Alifragkis, P., Denaxa, M., Karagogeos, D., Constanti, A., Lovell-Badge, R., Episkopou, V. (2005) Neuronal migration and ventral subtype identity in the telencephalon depend on SOX1 PLoS Biol. May 17;3(6):e186

Soares*, S., Traka*, M., vonBoxberg, Y., Karagogeos, D. and Nothias, F. (2005) Neuronal and glial expression of the adhesion molecule TAG-1 is regulated after peripheral nerve lesion or central neurodegeneration of adult nervous system. Eur. J. Neurosci. 21(5): 1169-1180
* Equal contribution

Llimargas, M. *, Strigini, M. *, Katidou, M., Karagogeos, D. and Casanova, J. (2004) Lachesin is a component of a septate junction based mechanism that controls tube size and epithelial integrity in the Drosophila tracheal system Development 131(1):181-90
* Equal contribution

Denaxa*, M., Pavlou*, O., Tsiotra, P., Papadopoulos, G.C., Liapaki, K., Theodorakis, K., Karagogeos+, D., and Papamatheakis+, J. (2003) The upstream regulatory region of the human homologue of the neuronal cell adhesion molecule TAG-1 gene contains elements driving neural specific expression in vivo Molecular Brain Research 118:91-101
* Equal contribution, + Equal senior contribution

Traka, M. *, Goutebroze, L. *, Denisenko, N., Bessa, M., Nifli, F., Havaki, S., Iwakura, Y., Fukamauchi, F., Watanabe, K., Girault, J.A. and Karagogeos, D. (2003) TAG-1 associates with Caspr-2 and is essential for the molecular organization of juxtaparanodal regions of myelinated fibers J Cell Biol 162 (6): 1161-1172
* Equal contribution

Karagogeos, D. (2003) Neural GPI-anchored cell adhesion molecules Invited Review Front. Biosci. 8, S13011-1320, Sept 1.
[PubMed#:pending]

Loberto, N., Prioni, S., Prinetti, A., Ottico, E., Chigorno, V., Karagogeos, D. and Sonnino, S. (2003) The adhesion protein TAG-1 has a ganglioside environment in the sphingolipid-enriched membrane domains of neuronal cells in culture. J. Neurochem. 85: 224-233

Theodorakis, K., Kyriakopoulou, K., Wassef, M., and Karagogeos, D. (2002) Novel sites of expression of the bHLH gene NSCL1 in the developing nervous system. Mech. Dev. 119S: 103-S106

Pavlou, O., Theodorakis, C., Falk, J., Kutsche, M., Schachner, M., Faivre-Sarrailh, C., and Karagogeos, D. (2002) Analysis of Interactions of the adhesion molecule TAG-1 and Its domains with other Immunoglobulin superfamily members Mol. Cell. Neurosci ., 20: 367-381

Traka, M. Dupree, J.L., Popko, B. and Karagogeos, D. (2002) The neuronal adhesion protein TAG-1 is expressed by Schwann cells and oligodendrocytes and is localized to the region of the node of Ranvier in myelinated fibers. J. Neurosci. 22(8):3016-3024

De Diego, I., Kyriakopoulou, K., Karagogeos, D. and Wassef, M. (2002) Multiple influences on the migration of precerebellar neurons in the caudal medulla Development 129: 297-306

Kyriakopoulou, K., DeDiego, I., Wassef, M. and Karagogeos, D. (2002) A combination of chain and neurophilic migration involving the adhesion molecule TAG-1 in the caudal medulla Development 129: 287-296

Denaxa, M., Chan, C-H., Schachner, M., Parnavelas, J.G. and Karagogeos, D. (2001) The adhesion molecule TAG-1 mediates the migration of cortical interneurons along the corticofugal fiber system Development 128: 4635-4644

Prinetti, A., Prioni, S., Chigorno, V., Karagogeos, D., Tettamanti, G. and Sonnino, S. (2001) Immunoseparation of sphingolipid-enriched membrane domains enriched in Src family protein tyrosine kinases and in neuronal adhesion molecule TAG-1 by anti-GD3 ganglioside monoclonal antibody J. Neurochem. 78 (5):1162-7

Stamataki, D., Kastrinaki, M-C., Mankoo, B.S., Pachnis, V. and Karagogeos, D. (2001) Homeodomain proteins Mox1 and Mox2 associate with Pax1 and Pax3 transcription factors. FEBS Lett., 499(3):274-8. Medline Abstract

Rallis, C., Stamataki, D., Pontikakis, S., Mankoo, B.S., and Karagogeos, D. (2001) Isolation of the avian homologue of the homeobox gene Mox2 and analysis of its expression pattern in developing somites and limbs. Mech. Dev. 104:121-124