Moflo Cell - SORTER
The facility is equipped with one research grade Cell-Sorting unit consisting of the DakoCytomation (now Beckman-coulter) MoFlo High-Performance Cell Sorter with the Summit software program (http://www.coulterflow.com/bciflow/instrumentsot.php).
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| MOFLO optical layout |
MoFlo - Sorter utilizes three Lasers: 1) UV- 355nm, 2) Blue - 488nm and 3) Red Diode - 643nm
which can measure up to 9 cellular markers according to Fig.1. Several common fluorochromes (FITC, R-PE, PerCp-Cy5.5, PE-Cy7, APC, APC - Cy5.5) and fluorescent GFP protein have been successfully used.
The unique configuration of the Lasers allows to simultaneous separation of 4 different populations with a purity greater than 99% and high recovery.
FACSCalibur
FACSCalibur is a bench-top cell analyzer, configured with two lasers:
1) 488 nm Blue-green Argon Ion laser
2) 633 nm Red Helium Neon laser
The FlowJo software is installed on a computer near to the FACSCalibur to analyze flow cytometry data.
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| Three fluorescence channels and two scatter channels are available from the 488 nm laser (FSC, SSC, FL1, FL2, FL3). One fluorescence channel is available from the 633 nm laser (FL4) |
