Publications
1. Alexandraki D. and Ruderman J. V. (1981) Sequence heterogeneity, multiplicity and organization of a- and ß-tubulin genes in sea urchins. Molec. Cell. Biol. 1, 1125-1137.
2. Ruderman J. V. and Alexandraki D. (1983) Organization and expression of the tubulin gene families in the sea urchin. J. Submicrosc. Cytol. 15, 349-352. (Paper presented at the International Conference on Development and Function in Cilia and Flagella, Siena, Italy, July 1982)
3. Alexandraki D. and Ruderman J. V. (1983) Evolution of a- and ß-tubulin genes as inferred by the nucleotide sequences of sea urchin cDNA clones. J. Molec. Evol. 19, 397-410.
4. Alexandraki D. and Ruderman J. V. (1985) Multiple polymorphic a- and ß-tubulin mRNAs are present in sea urchin eggs. Proc. Natl. Acad. Sci. USA 82, 134-138.
5. Alexandraki D. and Ruderman J. V. (1985) Expression of a- and ß-tubulin genes during development of sea urchin embryos. Develop. Biol. 109, 436-451.
6. Tzamarias D., Alexandraki D. and Thireos G. (1986) Multiple cis-acting elements modulate the translational efficiency of GCN4 mRNA in yeast. Proc. Natl. Acad. Sci. USA 83, 4849-4853.
7. Oliver S. G., ...Alexandraki D., ...Galland P., ...Thireos G., Tzermia M., ...and Sgouros J. G. (1992) The complete DNA sequence of yeast chromosome III. Nature 357, 38-46.
8. Tzermia M., Horaitis O. and Alexandraki D. (1994) The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein. Yeast 10, 663-679.
9. Alexandraki D. and Tzermia M. (1994). Sequencing of a 13.2 kb segment next to the left telomere of yeast chromosome XI revealed five open reading frames and recent recombination events with the right arms of chromosomes III and V. Yeast 10, S81-S91.
10. Georgatsou E. and Alexandraki D. (1994) Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae. Molec. Cell. Biol. 14, 3065-3073.
11. Dujon B., ...Alexandraki D., ...Horaitis O., ...Tzermia M.,... and Becker I. (1994) The complete DNA sequence of chromosome XI of Saccharomyces cerevisiae (666 kb). Nature 369, 371-378.
12. Galibert F., Alexandraki D.,...Katsoulou C.,...Tzermia M.,..and Karpfinger-Hartl L. (1996) Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X. EMBO J. 15, 2031-2049.
13. Katsoulou C., Tzermia M., Tavernarakis N. and Alexandraki D. (1996) Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI. Yeast 12, 787-797.
14. Tavernarakis N., Alexandraki D., Liodis P., Tzamarias D. and Thireos G. (1996) Gene overexpression reveals alternative mechanisms that induce GCN4 mRNA translation. Gene 179, 271-277.
15. Georgatsou E., Mavrogiannis L. A., Fragiadakis G. S. and Alexandraki D. (1997) The yeast Fre1p/Fre2p cupric reductases facilitate copper uptake and are regulated by the copper-modulated Mac1p activator. J. Biol. Chem. 272, 13786-13792.
16. Tzermia M., Katsoulou C. and Alexandraki D. (1997) Sequence Analysis of a 33.2 kb Segment from the Left Arm of Yeast Chromosome XV Reveals 8 Known Genes and 10 New Open Reading Frames Including Homologues of ABC Transporters, Inositol Phosphatases and Human ESTs. Yeast 13, 583-589.
17. Dujon, B., ...Alexandraki, D.,...Katsoulou, C.,...Tzermia,...and Kleine, K. (1997). The complete nucleotide sequence of Saccharomyces cerevisiae chromosome XV. Nature 387 (Supp.), 98-102.
18. Goffeau A., … Alexandraki D., … Horaitis O., … Thireos G. … Tzermia M,… (1997) The yeast genome directory. Nature 387 (suppl.).
19. Voutsina A., Riva M., Carles C. and Alexandraki D. (1999) Sequence divergence of the RNA polymerase shared subunit ABC14.5 (Rpb8) affects selectively the RNA polymerase III assembly in Saccharomyces cerevisiae. Nucl. Acids Res. 27, 1047-1055.
20. Georgatsou E. and Alexandraki D. (1999) Regulated Expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu Reductase Related Genes. Yeast 15, 573-584.
21. Voutsina A., Fragiadakis G. S., Boutla A. and Alexandraki D. (2001) The second cysteine-rich domain of Mac1p is a potent transactivator that modulates DNA binding efficiency and functionality of the protein. FEBS Lett. 494, 38-43.
22. Georgakopoulos T., Koutroubas G., Vakonakis I., Tzermia M., Prokova V. Voutsina A. and Alexandraki D. (2001) Functional analysis of the Saccharomyces cerevisiae YFR021w/ YGR223c/YPL100w ORF family suggests relations to mitochondrial/peroxisomal functions and amino acid signaling pathways. Yeast 18, 1155-1171.
23. Fragiadakis G.S., Tzamarias D. and Alexandraki D. (2004) Nhp6 facilitates Aft1 binding and Ssn6 recruitment both essential for FRE2 transcriptional activation. EMBO J. 23, 333-342.
24. Voutsina A., Gkouskou K . Alexandraki D. Hir1 and Ssn6 coregulators function in combination on CTR1 transcription (in preparation)
25. Voutsina A., Sereti K, Kotsis D., Koutsododis G., Tzermia M, Georgakopoulos T. and Alexandraki D. A functional analysis of Siw14 protein tyrosine phosphatase paralogues in S. cerevisiae: interelations and function on kinetochores (in preparation)
SCIENTIFIC EDITOR IN BOOK
Alexandraki D. (1997) Scientific Editor for the translation in Greek
of the book: “A Genetic Switch, Phage ? and Higher Organisms”
by Mark Ptashne,1992, Blackwell Scientific Publications & Cell Press
(Translation by C. E. Kazlaris, University Press of Crete) (
http://www.cup.gr )
REVIEW PAPER IN BOOK
Alexandraki D. (2002) From DNA Sequence to Function, in Genome Sequencing
and Comparative Analysis Eds A. S. Tsaftaris & A. N. Polidoros,
pp 133-158.
PAPERS PRESENTED IN CONFERENCES/ WORKSHOPS
1. Alexandraki D. and Ruderman J. V. Isolation and characterization
of cDNA clones encoding sea urchin a- and ß-
tubulins. J. Cell. Biol. 83, 342a. Cell
Biology Meetings, Toronto, Canada, November 1979.
2. Alexandraki, D. and Ruderman J. V. Analysis of tubulin genes and
transcripts in the sea urchins by the use of cloned cDNA and genomic
tubulin sequences. Symposium on Organization
and Expression of the Genes for Tubulins, Marine Biological Laboratory,
Woods Hole, MA, USA, July 1981.
3. Alexandraki D. and Ruderman J. V. Organization and expression of
tubulin gene families in the sea urchin. The 41st Annual Symposium
of the Society for Developmental Biology on Gene Structure and Regulation
in Development, Harvard University, Cambridge, MA, USA, June
1982.
4. Alexandraki D. Organization and expression of a- and ß-tubulin
genes in the sea urchin Lytechinus pictus. Symposium
on Developmental Biology of the Sea Urchin Embryo, Marine Biological
Laboratory, Woods Hole, MA, USA, August 1982.
5. Thireos G., Alexandraki D. and Tzamarias D. Involvment of cis-
and trans-acting elements in translational regulation. UCLA
Symposium on Molecular Approches to Developmental Biology,
Keystone, CO, USA, April 1986.
6. Alexandraki, D. and Thireos G. Isolation of a new gene (TAR1)
involved in the translation of the HIS3 mRNA in Saccharomyces
cerevisiae. Yeast 4, special issue, s507.
14th International Conference on Yeast Genetics and Molecular
Biology, Helsinki, Finland, August 1988.
7. Alexandraki D., Gallant P. and Thireos G. Sequencing part of chromosome
III from S. cerevisiae. Meeting on Genome
Mapping and Sequencing, Cold spring Harbor Laboratory,
CSH, New York, USA, April 1989.
8. Alexandraki, D. and Thireos G. Sequencing part of chromosome III
from S. cerevisiae. BAP meeting on the Sequencing of
the Yeast Chromosome III. Tutzing Obb., FRG, November 1989.
9. Thireos G. Alexandraki D., Dialynas G., Krupitza G., Maniataki E.,
and Tzamarias D. Translational regulation of GCN4 mRNA: in
vivo and in vitro approaches. NATO/EEC Workshop
on Post-transcriptional Control of Gene Expression, Goslar,
West Germany, April 1990.
10. Thireos G., Alexandraki D., Georgakopoulos T., Dialynas G., Krupitza
G., Maniataki E., Roussou I., Tzamarias D. and Tzortzakis N. Translational
regulation of GCN4 mRNA and the control of protein synthetic
rates in yeast. I.C.G.E.B. Colloquium on “Eukaryotic Gene
Regulation and Expression”, Heraklio, Crete, Greece,
May 1990.
11. Alexandraki D., Tzamarias D. and Thireos G. (1990). High levels
of uncharged tRNAs override the known signalling pathway for the translational
activation of the GCN4 mRNA. Yeast
6, special issue, s390. 15th International Conference on Yeast
Genetics and Molecular Biology, The Hague, The Netherlands,
July 1990.
12. Alexandraki D., Tzermia M. and Thireos G. Expression of the YCL49
sequence of chromosome III. 2nd meeting of the European Network
in charge of the “Sequencing the Yeast Chromosome III”.
The Hague, The Netherlands, July 1990.
13. Alexandraki D., Tzermia M. and Thireos G. Sequencing of the pEKG100
fragment of yeast chromosome XI. 1st BRIDGE meeting on “Sequencing
the Yeast Chromosomes II & XI”. Brugge (B), Belgium,
September 1991.
14. Thireos G., Alexandraki D., Dialynas G., Georgakopoulos T., Georgatsou
E., Maniataki E. and Tavernarakis N. Transcriptional and translational
control mechanisms: the GCN4 model in yeast. 9th Balkan
Biochemical and Biophysical Days, May 1992.
15. Thireos G., Alexandraki D., Georgakopoulos T., Maniataki E. and
Tavernarakis N. Transcriptional and translational control mechanisms:
the GCN4 model in yeast. 16th International Conference
of Yeast Genetics and Molecular Biology, Vienna, Austria, August
1992.
16. Alexandraki D., Tzermia M., Horaitis O., Georgatsou E. and Thireos
G. Sequencing and analysis of the cosmid clone pEKG100 of chromosome
XI. 2nd BRIDGE meeting on “Sequencing the Yeast Chromosomes
II & XI”. Munich, Germany, October 1992.
17. Thireos G., Alexandraki D., Georgakopoulos T., Maniataki E. and
Tavernarakis N. Translational and transcriptional regulation of gene
expression: The GCN4 model in yeast. Workshop on “Control
of Gene Expression in Yeast”. Madrid, Spain, December
1992.
18. Tzermia M., Tavernarakis N., Thireos, G. and Alexandraki, D. Sequencing
of a 6 kb segment from cosmid pUKG040 and of the left telomeric region
of chromosomeXI: BRIDGE-BIOTECH on “Yeast Genome Sequencing
Meeting”. Louvain-La-Neuve, Belgium, April 1993.
19. Tzermia M., Katsoulou C. and Alexandraki D. Sequencing of the overlapping
cosmids 7 and 31 of yeast chromosomeX : Biotechnology, “Yeast
Genome Sequencing Network”, Manchester, UK, 26/2-1/3
1994.
20. Alexandraki D., Klinakis A. and Georgatsou E. The involvement of
stress-related transcription factors in the iron-dependent regulation
of the FRE2 ferric reductase gene of Saccharomyces cerevisiae.
In ”Metal and Oxygen Regulation of Gene
Expression”, Keystone Symbosia on Molecular and Cellular Biology,
Park City, Utah, USA, March 18-241995.
21. Georgatsou E., Mavrogiannis L. and Alexandraki D. The FRE1
and FRE2 Saccharomyces cerevisiae genes essential
for iron uptake participate also in copper metabolism. In ”Metal
and Oxygen Regulation of Gene Expression”, Keystone Symbosia on
Molecular and Cellular Biology, Park City, Utah, USA, March
18-24 1995.
22. Katsoulou C., Tzermia M. and Alexandraki D. Complete sequencing
of a 40.7 kb segment from the overlapping cosmids pEJ007and pEJ031 of
yeast chromosome X and sequencing in progress of cosmid pEOA1044 of
chromosome XV: Biotechnology, “Yeast Genome Sequencing
Network”, Lisboa, Portugal, 8-10/6 1995.
23. Tzermia M., Katsoulou C., Tavernarakis N. and Alexandraki D. The
complete sequence of a 40.7 kb segment located on the left arm of yeast
chromosome X identified 11 known genes and revealed 15 new open reading
frames including homologues of other yeast hypothetical proteins. 17th
International Conference of Yeast Genetics and Molecular Biology,
Lisboa, Portugal, 10-16/6 1995.
24. Voutsina A. and Alexandraki D. Overexpression of the S. pombe
RPB8 cDNA affects the translational machinary in S. cerevisiae
by competing the endogenous subunit for the assembly of the RNA polymerase
III complex. 17th International Conference of Yeast Genetics
and Molecular Biology, Lisboa, Portugal, 10-16/6 1995.
25. Georgatsou E., Mavrogiannis L., Frangiadakis G, Klinakis A. and
Alexandraki D. The Saccharomyces cerevisiae ferric reductase
genes FRE1 and FRE2 are distinctly regulated by iron,
copper and stress related factors. 17th International Conference
of Yeast Genetics and Molecular Biology, Lisboa, Portugal,
10-16/6 1995.
26. Georgatsou E., Frangiadakis G., Mavrogiannis L. and Alexandraki
D. Plasma membrane ferric reductases of Saccharomyces cerevisiae:
Involvement in metal uptake and homeostasis. Biochemical Society
Conference. Athens, Greece, January 1996.
27. Alexandraki D., Jauniaux J.-C. and Sunnerhagen P. Gene interactions:
two-hybrid approach of functional analysis for the newly identified
yeast genes. 1st EUROFAN, Biotechnology meeting. Louvain-La-Neuve,
Belgium, March 28-31 1996.
28. Alexandraki D., Jauniaux J.-C. and Sunnerhagen P. Gene interactions:
two-hybrid analuses. 2nd EUROFAN, Biotechnology
meeting. Manchester-UMIST, UK, February 20-23 1997.
29. Georgatsou E., Fragiadakis G., Boutla A. and Alexandraki D. Metal
homeostasis in Saccharomyces cerevisiae: Regulated gene expression
of metal reductases. Biochemical Society conference.
Athens, Greece, December 1997.
30. A. Voutsina, T. Georgakopoulos, A. Boutla, M. Tzermia, E. Georgatsou
and D. Alexandraki. Gene Disruptions and Two-Hybrid Screens. EUROFAN’98,
The Third Meeting. Noordwijkerhout, NL, 16-19 May 1998.
31. A. Voutsina, E. Georgatsou, T. Georgakopoulos, M. Tzermia, A. Boutla
and D. Alexandraki. Functional analysis of newly identified yeast open
reading frames. Functional Genomics. The Luunberg Institute
and The Technology Link Foundation of Goeteborg. Sweden, 27-28
August 1998.
32. T. Georgakopoulos, A. Voutsina, I. Vakonakis, D. Kotsis, M. Tzermia,
G. Koutsododis and D. Alexandraki. Functional analysis of gene families
by gene disruption, overexpression and two-hybrid protein-protein interactions.
EUROFAN 1999, Satellite meeting of XIX International Conference
on Yeast Genetics and Molecular Biology. Rimini, Italy, May
1999.
33. D. Alexandraki. a) From DNA sequence to function. b) Functional
analysis of the yeast Saccharomyces cerevisiae genome. Genome
Sequence and Comparative Analysis: Advanced Workshop in Biotechnology,
organized by the European Commission, in the framework of the Biotechnology
R&D Programme (1999 – 2000), Thessaloniki, Greece
21-25 November 1999. (presentation and review paper).
34. T. Georgakopoulos, G. Koutroubas, A. Voutsina, G. Koutsododis, V.
Prokova, M. Tzermia, and D. Alexandraki. YFR021w and YPL100w
ORFs of a three-member family are involved in the mitochondrial retrograde
and aminoacid-sencing pathways. All four members of the SIW14
tyrosine phosphatase gene family are possibly involved in MAP kinase
signalling pathways. EUROFAN 2000. Salamanca, September
28-30 2000.
35. D. Alexandraki. Systematic genomic functional analysis: The yeast
model. EEBE (Biological Society) 23rd Conference, Chios,
Greece May 24-27, 2001.
36. T. Georgakopoulos, A. Voutsina, G. Koutroubas, M. Tzermia, I. Vakonakis,
D. Kotsis, G. Koutsododis, V. Prokova, G. S. Fragiadakis and D. Alexandraki.
A functional analysis of paralogous open reading frames in Saccharomyces
cerevisiae. EEBE (Biological Society) 23rd Conference,
Chios, Greece May 24-27, 2001.
37. M. Kokkinaki, T. Georgakopoulos, M.-D. Bazopoulou, D. Alexandraki
and N. K. Moschonas. Functional analysis of the mouse NEURALIZED
gene with the yeast two-hybrid system. EEBE (Biological Society)
23rd Conference, Chios, Greece May 24-27 2001.
38. A. Voutsina, K. Gkouskou, Y. Kagiampakis, G.S. Fragiadakis and D.
Alexandraki. Mac1p interacting proteins reveal novel copper-independent
Mac1p regulation. The XXth International Conference on Yeast
Genetics and Molecular Biology. Prague, Czech Republic, August
26-31, 2001.
39. G.S. Fragiadakis, E. Georgatsou, A. Voutsina and D. Alexandraki.
THE HMG PROTEINS Nhp6A/B PROMOTE THE Aft1p-MEDIATED TRANSCRIPTIONAL
ACTIVATION OF FRE2 FERRIC REDUCTASE GENE IN Saccharomyces
cerevisiae. Cold Spring Harbor Laboratory Meeting in Mechanisms
of Eukaryotic Transcription, August 29-September 2, 2001.
40. A. Voutsina, G.S. Fragiadakis and D. Alexandraki. Mac1p modulation
by copper and protein interactions in Saccharomyces cerevisia.
SMYTE 19 (Small Meeting on Yeast Transport and Energetics),
Chania, Crete, Greece, September 14-17, 2001.
41. D.Alexandraki. Molecular information and perspectives. P.E.V. (Union
of Greek Biologists) 2nd Greek Meeting on Health.
Postgenomic Era: Genomic Applications and Perspectives.
Athens October 5-6 2001.
42. Kokkinaki M., Georgakopoulos T., Bazopoulou M.-D., Koutelou E.,
Pavlopoulos E., Alexandraki D. and Moschonas N. K. FUNCTIONAL ANALYSIS
OF THE MOUSE NEURALIZED GENE. 53rd Conference of EEBMB,
Athens, Greece, December 13-15 2001.
43. Fragiadakis G. S., Tzamarias D. and Alexandraki D. Ssn6p (Cyc8p)
positively regulates ferric reductase gene transcription in Saccharomyces
cerevisiae. EURESCO Conferences, Gene transcription in
yeast, Castelvecchio Pascoli, Italy, 31 May-05 June 2002.
44. Voutsina A., Fragiadakis G. S. and Alexandraki D. PROTEIN –PROTEIN
INTERACTIONS IN METALLOREGULATED TRANSCRIPTIONAL COMPLEXES IN SACCHAROMYCES
CEREVISIAE EEBE (Biological Society)
25th Conference, Mytilini, Greece, May 29-June 1st 2003.
45. G. S. Fragiadakis, D. Tzamarias and D. Alexandraki. Nhp6 architectural
factor and Ssn6/Cyc8 co-repressor distinctly and cooperatively potentiate
Aft1-mediated transcriptional activation on FRE2 promoter in
Saccharomyces cerevisia. The XXIth International Conference
on Yeast Genetics and Molecular Biology. Goeteborg, Sweden,
July 7-12, 2003.
46. Kokkinaki M., Koutelou E., Bazopoulou M.-D., Georgakopoulos T.,
Alexandraki D., Pavlopoulos E. and Moschonas N. K. Neuralized has ligase
E3 activity for protein sumoylation and is part of the ubiquitination
complex. EEBMB (Biochemical Society Conference) Athens,
November 13-15 2003.
47. Voutsina A., Gkouskou K. and Alexandraki D. Hir1 and Ssn6 coregulators
function in combination on the Mac1-dependent CTR1 transcription.
The XXII International Conference on Yeast Genetics & Molecular
Biology. Bratislava, Slovak Republic, August 7-12 2005.
