The High content screening facility (HCS) of IMBB has started its operation in September 2015 and it is equipped with a HCS Microscope Operetta (Perkin Elmer). HCS is one of the fastest growing microscopy fields in cell biology and combines the automation of fluorescence microscopy coupled with quantitative image analysis in large scale applications. The Image based screening measures a variety of phenotypes in cell and whole organisms. It is the choice of microscopy for studies aim to track changes on cellular morphology and behaviour due to the effect of a drug or in RNAi screening. It is also used to measure: protein expression and translocation, fluorescence redistribution within the cell, apoptosis, proliferation, viral infection, organelle structure, cell and organ morphology, neurite outgrowth, signalling pathway analysis etc.