The Fly/Insect facility supports research on Drosophila and other insect species, including species of medical importance (mosquito) and agricultural pests (medfly, olive-fly, flour beetle). It is a multi-user facility for insect genetics and transgenesis, equipped with a state-of-the-art system for embryo injection. In addition to routine transgenesis of Drosophila melanogaster, the facility has produced transgenic lines of Ceratitis caritata (medfly), Bactrocera olea e (olive fly) and Tribolium castaneum (flour beetle). Furthermore, cytoplasmic transfers between different dipteran species for the propagation of bacterial symbionts (Wolbachia) have been successfully carried out.
The facility is built around the central Flyroom, a large lab with four work stations equipped with CO2, anaesthetization stages, stereoscopes and cool light sources.
The Flyroom is surrounded by three walk-in environmental chambers with controlled temperature, humidity and light/ dark cycle. An isolated two-room space is dedicated to rearing Anopheles gambiae mosquitoes. Within the mosquito insectarium, infections with the rodent malaria parasite Plasmodium berghei are performed. Dark chambers for fluorescence microscopy complete the setup.
Satellite facilities are the microinjection lab and a fly-food preparation kitchen.
Leica Stereomicroscopes MZ 6, MZ 8, MZFL III
Leitz Microscope GMBH
Leitz DIAPLAN microscopes with Nomarski and fluorescence optics
NARISHIGE Motor Drive Microinjector IM - 300
SUTTER INSTRUMENT Flaming/ Brown Micropipette Puller P- 97
9. Drummond NanojectII microinjection system
10. Cool light sources, cabinet incubators, balances, refrigerators and other small equipment.
• Centralized production of Drosophila, medfly and olive-fly food
• Centralized CO 2 delivery system
• Stock maintenance by specialized personnel
• Embryo microinjection by specialized personnel
• Pest management against bacterial, fungal and mite infestation
Head technician - microinjection specialist
|Telephone number||+30-2810-391190, 391192|
|Room Number||A010, A054|
Fly kitchen personnel
Christos Delidakis (Drosophila)
Domna Karagogeos (Neuronal Development, Myelination)
Michalis Averof (Evolution of development and morphology)
Kriton Kalantidis (RNA silencing)
Christos (Kitsos) Louis (the Anopheles mosquito and parasites)
Thanasis Loukeris (Insect Molecular Biology)
Contact for the mosquito facility
• Loukeris T.G., I. Livadaras, B. Arcà, S. Zabalou and C. Savakis. 1995. Gene transfer into the Medfly, Ceratitis capitata, with a Drosophila hydei transposable element. Science 170: 2002-2005.
• Gubb, D., A. Zacharopoulou, I. Livadaras, P. Gourzi, J. Roote, and C. Savakis. 1998. Recovery of a marked translocation strain that will facilitate the isolation of balancer chromosomes in the Mediterranean fruit fly, Ceratitis capitata. Genome 41: 256-265.
• Ligoxygakis, P., Bray, S., Apidianakis, Y. and Delidakis C. 1999. Ectopic expression of E(spl) genes has differential effects on different cell fate decisions and underscores the biphasic requirement for Notch activity in wing margin establishment in Drosophila . Development 126, 2205-2214.
• Spidianakis, Y., Nagel, A.C., Preiss, A. and Delidakis, C. 1999. The m4 and ma genes of the E(spl)-Complex antagonize Notch mediated lateral inhibition. Mech. Dev ., 86, 39-50.
• Christophides G.K., I. Livadaras, C. Savakis and K. Komitopoulou. 2000. Two medfly promoters that have originated by a recent duplication drive distinct sex, tissue and developmental expression patterns. Genetics. 156:173-182.
• Apidianakis, Y., Grbavec, D., Stifani, S. and Delidakis, C. 2001. Groucho mediates a Ci-independent mechanism of hedgehog repression in the anterior wing pouch. Development 128, 4361-4370.
• Ligoxygakis P, Strigini M, and Averof M (2001) Specification of left-right asymmetry in the embryonic gut of Drosophila . Development 128: 1171-4.
• Boutla, A., Delidakis, C., Livadaras I. , Tsagris, M. and Tabler, M. 2001. Short 5'-phosphorylated double-stranded RNAs induce RNA interference in Drosophila . Curr. Biol. 11, 1776-1780.
• Pavlopoulos, E., Pitsouli, C., Klueg, K., Muskavitch, M.A.T., Moschonas, N.K. and Delidakis, C. 2001. neuralized encodes a peripheral membrane protein involved in Delta signalling and endocytosis. Dev. Cell 1, 807-816
• Kapetanaki M.G., T.G. Loukeris, I. Livadaras and C. Savakis. 2002. High frequencies of Minos transposon mobilization are obtained in insects by using in vitro synthesized mRNA as a source of transposase. Nucleic Acids Res. 30: 3333-3340.
• Giagtzoglou, N., Alifragis, P., Koumbanakis, K.A. and Delidakis, C. 2003. Two modes of recruitment of E(spl) repressors onto target genes. Development 130, 259-270.
• Boutla, A., Delidakis, C. and Tabler, M. 2003. Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes. Nucleic Acids Res , 31, 4973-4980.• Zabalou, S., M. Riegler, M. Theodorakopoulou, C. Stauffer, C. Savakis and K. Bourtzis. 2004. Wolbachia-induced cytoplasmic incompatibility as a means for insect pest population control. Proc. Natl. Acad. Sci. USA. 101:15042-15045.
• Llimargas M, Strigini M, Katidou M, Karagogeos D, and Casanova J. 2004. Lachesin is a component of a septate junction based mechanism that controls tube size and epithelial integrity in the Drosophila tracheal system. Development 131: 181-90.
• Giagtzoglou, N., Koumpanakis, K.A., Fullard, J.F., Zarifi, I. and Delidakis, C. 2005. Role of the Sc C-terminus in transcriptional activation and E(spl) repressor recruitment. J. Biol. Chem. 280, 1299-305
• Pitsouli, C. and Delidakis, C. 2005. The interplay between DSL proteins and ubiquitin ligases in Notch signaling. Development 132, 4041-4050.
• Strigini M, Cantera R, Morin X, Bastiani MJ, Bate M and Karagogeos D. 2006. The IgLON protein Lachesin is required for the blood-brain barrier in Drosophila. Molecular and Cellular Neuroscience 32: 91-101.
• Zabalou S, Apostolaki A, Pattas S, Veneti Z, Paraskevopoulos C, Livadaras I, Markakis G, Brissac T, Merçot H, Bourtzis K. 2008. Genetics 178, 2145-60.
• Monastirioti, M., Giagtzoglou, N,. Koumbanakis, K.A., Zacharioudaki, E., Deligiannaki, M., Wech, I., Almeida, M., Preiss, A., Bray, S. and Delidakis, C. 2010. Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis. Development 137, 191-201