The Electrophysiology Unit was established in 2007 with the generous support of the A.G. Leventis Foundation, and has since been significantly upgraded. The Unit contains all the necessary equipment for performing both field and patch-clamp recordings from brain slices and/or cell cultures.
The electrophysiology unit is located in the second level of the Biology department, Room 2.228 (phone number: +30 2810394051).
Vibration isolation: Vibraplane 9100/9200 series (Kinetic Systems), with Faraday cage
Microscope: Axioskop 2FS (Zeiss)
Camera: Black and white camera for DIC-IR and fluorescence optics (Scientifica)
Manipulators: MPC-325-2 (Sutter)
Amplifier: BVC-700 (Dagan)
Amplifier: Axopatch-200B (Molecular Devices)
A-D board: ITC-18 (Instrutech)
Microscope accessories required for electrophysiological recordings: XY-translational stage (Scientifica) And aluminum bases for tissue chamber and manipulators (Scientifica and IESL workshop)
Stimulator: A320RC (Hybrid stimulator/isolator)
Perfusion system: Vaccum pump to enable fluid flow and custom-made tubing system
Acquisition and analysis software: IgorPro (Wavemetrics, Inc)
Glass puller: P-97 (Sutter)
The facility can be used to design and perform several electrophysiological protocols from a variety of preparations
a) short-term and long-term plasticity experiments from brain slices of various cortical areas, the hippocampus or amygdala
b) current-clamp recordings to study neuronal excitability, spontaneous or evoked synaptic responses from brain slices with pharmacological applications if necessary.
c) recordings can also be performed from YFP or GFP- labeled cells, either from brain slices or neuronal cultures
d) voltage-clamp recordings to study spontaneous excitatory and/or inhibitory activity from brain slice or cell cultures
Other specialized protocols could also be performed upon request, given the suitability of existing equipment.
1. Age-dependent changes in neuronal excitability of pyramidal neurons and interneurons (Sidiropoulou/Karagogeos)
2. Mechanisms of synaptic plasticity processes in the prefrontal cortex (Sidiropoulou)
3. Adaptations in network activity and synaptic properties in mice with decreased number of interneurons (Karagogeos/Sidiropoulou)
4. Adaptations in neuronal excitability and synaptic plasticity in mouse models of schizophrenia-like symptoms (Sidiropoulou)
5. Effect of neurosteroids on short-term and long-term plasticity processes in the hippocampus (Gravanis/Charalampopoulos/Sidiropoulou)
1. X. Konstantoudaki, K. Chalkiadaki, S. Tivodar, D. Karagogeos and K. Sidiropoulou, Impaired synaptic plasticity in the prefrontal cortex of mice with developmentally decreased numbers of interneurons, Neuroscience. 2016 Feb 26. pii: S0306-4522(16)00188-3. doi: 10.1016/j.neuroscience.2016.02.048.
2. Elena Georgiou, Kyriaki Sidiropoulou, Jan Richter, Christos Papaneophytou, Irene Sargiannidou, Alexia Kagiava, Georg von Jonquieres, Christina Christodoulou, Matthias Klugmann, Kleopas A. Kleopa Gene therapy targeting oligodendrocytes provides therapeutic benefit in a leukodystrophy model, under revision
Representative poster presentations in scientific meetings
1. K. Kalemaki, K. Sidiropoulou and D. Karagogeos (2016) The contribution of developmental inhibitory changes in early postnatal circuits of the prefrontal cortex, AREADNE 2016, Santorini, Greece
2. X. Konstantoudaki, K. Chalkiadaki and K. Sidiropoulou (2015), Decreased prefrontal cortical function, synaptic plasticity and dendritic spine density in adolescent mice compared to adults, EBBS-EBPS joint meeting, Verona, Italy
3. X. Konstantoudaki, V. Ioakimides and K. Sidiropoulou (2015), Enhanced long-term potentiation underlies the effects of working memory training, EBBS-EBPS joint meeting, Verona, Italy
4. X. Konstantoudaki, S. Tivodar, D. Karagogeos and K. Sidiropoulou (2013) Age-dependent changes in prefrontal cortical function: The role of inhibition, European Brain and Behavior Society meeting, Munich, Germany
5. X. Konstantoudaki, S. Tivodar, K. Kalemaki, V. Sakka, D. Karagogeos and K. Sidiropoulou (2012) Behavioral and plasticity deficits in mice with reduced inhibition, FENS forum, Barcelona, Spain
6. O. Tzortzi, C. Papantoniou, A. Chovsepian, P. Iordanidou, X. Konstantoudaki, P. Efstathopoulos, I. Charalampopoulos, A. Gravanis and K. Sidiropoulou (2012) Effect of neurosteroids on contextual fear memory and hippocampal long-term potentiation, Hellenic Society for molecular biology and biochemistry (EEMBB) meeting, Heraklio, Greece
Publications in preparation
• X. Konstantoudaki, K. Chalkiadaki, S. Tivodar, D. Karagogeos and K. Sidiropoulou, Alterations in synaptic plasticity of layer II prefrontal cortex underlie the development of emotional processes and cognitive functions from early adolescence to adulthood
• X. Konstantoudaki, K. Chalkiadaki, S. Tivodar, D. Karagogeos and K. Sidiropoulou, Cellular mechanisms underlying co-morbidity of anxiety and epilepsy in mice with decreased number of interneurons
Pictures from experiments
Recording from a pyramidal neurons in the amygdala visualized using DIC optics
Potentiated fEPSP (purple) following tetanic stimulation compared to baseline fEPSP (black) in the CA1 area
Action potentials in response to step pulse stimulation from the a pyramidal neuron in the prefrontal cortex