BD FACS AriaTM III Cell Sorter
The BD FACSAriaTM III flow cytometer is a high-speed fixed-alignment benchtop cell sorter (https://www.flowcytometry.bmc.med.uni-muenchen.de/best-practice-faq/acquisition/facsaria_user_guide.pdf).
With its fixed-optics design and digital electronics, the BD FACSAriaTM III flow cytometer enables multicolor analysis of up to 18 fluorescent and two scatter options at a time. Nearly all cytometer functions are operated from within BD FACSDivaTM software.
The BD FACSAriaTM III flow cytometer utilizes four lasers:
- Violet - 405nm, 2) Blue - 488nm, 3) Yellow Green - 561nm and 4) Red Diode - 633nm.
Several common fluorochromes (FITC, Alexa Fluor 488, R-PE, PE-Texas Red, PerCP, PerCp-Cy5.5, PE-Cy7, APC, APC - Cy5.5, BD Horison VPD450, BD Horison Brilliant Violet 421, Pacific Blue, DAPI etc) and fluorescent GFP protein have been successfully used.
The unique configuration of the Lasers allows to simultaneous separation of 4 different populations with purity greater than 99% and high recovery.
