imbb logo for mobile
 
Konstantinos Theodorakis (Staff Scientist - Karagogeos Lab)

Konstantinos Theodorakis (Staff Scientist - Karagogeos Lab)

Senior Staff Scientist
Email address
This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone
+30-2810-394547 (office)
  
+30-2810-394543 (lab)
Fax
-
 

My scientific interests focus on the biology of the nervous system and especially on neuronal development and axoglial interactions during myelination.
I have participated in several research projects related to myelination and demyelinating diseases. Since 2018 we have initiated two research projects concerning the use of biobased and biocompatible nanomolecules for drug encapsulation and drug delivery. Our main goal is the use of novel nanocarriers to deliver known agents that could improve remyelination in vivo.
During my scientific career I have worked with multiple experimental models, such as chick embryos, mice, and various mammalian neuronal and other cell lines. My technical expertise includes several molecular and biochemical techniques, like gene cloning, PCR, mouse genetics, protein and mRNA expression analysis (immunohistochemistry/immunofluorescence and in situ hybridization respectively), real time PCR, cell culture, tissue cultures, brain slice electroporation, FACS, in utero electroporation etc.

 

Savvaki M, Kafetzis G, Kaplanis SI, Ktena N, Theodorakis K, Karagogeos D. (2021) Neuronal, but not glial Contactin 2 negatively regulates axon regeneration in the injured adult optic nerve. Eur J Neurosci. 53:1705-1721.

Koumentakou I, Terzopoulou Z, Michopoulou A, Kalafatakis I, Theodorakis K, Tzetzis D, Bikiaris D. (2020) Chitosan dressings containing inorganic additives and levofloxacin as potential wound care products with enhanced hemostatic properties. Int J Biol Macromol. 162:693-703.

Kastriti ME, Stratigi A, Mariatos D, Theodosiou M, Savvaki M, Kavkova M, Theodorakis K, Vidaki M, Zikmund T, Kaiser J, Adameyko I, Karagogeos D. (2019) Ablation of CNTN2+ Pyramidal Neurons During Development Results in Defects in Neocortical Size and Axonal Tract Formation. Front Cell Neurosci. 13:454.

Savvaki M, Theodorakis K, Zoupi L, Stamatakis A, Tivodar S, Kyriacou K, Stylianopoulou F, Karagogeos D. (2010) The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS. J Neurosci. 30:13943-1354.

Theodorakis K, Kyriakopoulou K, Wassef M, Karagogeos D. (2002) Novel sites of expression of the bHLH gene Nscl1 in the developing nervous system. Mech Dev. 119 Suppl 1:S103-6.

1.    Chantziou, A., Theodorakis, K., Polioudaki, H., de Bree, E., Kampa, M., Mavroudis, D., Castanas, E., Theodoropoulos, A. P.. Glycosylation modulates plasma membrane trafficking of CD24 in breast cancer cells. International Journal of Molecular     Sciences, 2021 July 29;22(15):8165..

2.    Savvaki, M.,Kafetzis, G., Theodorakis, K. and Karagogeos, D.Neuronal, but not glial Contactin 2, negatively regulates axon regeneration in the injured adult optic nerve. European Journal of Neuroscience, 2021 Mar;53(6):1705-1721.

3.    Koumentakou, I., Terzopoulou, Z., Michopoulou, A., Kalafatakis, I., Theodorakis, K., Tzetzis, D., Bikiaris, D. Chitosan dressings containing inorganic additives and levofloxacin as potential wound care products with enhanced hemostatic properties. International Journal of Biological Macromolecules, 2020 Nov. 1;162:693-703

4.    Kastriti, M.E., Stratigi, A., Mariatos, D., Theodosiou, M., Savvaki, M., Kavkova, M., Theodorakis, K., Vidaki, M., Zikmund, T., Kaiser, J., Adameyko, I., Karagogeos, D. Ablation of CNTN2+ Pyramidal Neurons During Development Results in Defects in Neocortical Size and Axonal Tract Formation. Frontiers in Cellular Neuroscience, Volume 13:454, November 2019.

5.    Papadogkonaki, S., Theodorakis, K., Thermos, K. Endogenous and synthetic cannabinoids induce the downregulation of cannabinoid CB1 receptor in retina. Experimental Eye Research,Volume 185, August 2019

6.    Tivodar, S., Kalemaki, K., Kounoupa, Z., Vidaki, M., Theodorakis, K., Denaxa, M., Kessaris, N., de Curtis, I., Pachnis. V., Karagogeos, D. Rac-GTPases Regulate Microtubule Stability and Axon Growth of Cortical GABAergic Interneurons. Cereb Cortex. 2015 Sep;25(9):2370-82.

7.    Savvaki, M., Theodorakis, K., Zoupi, L., Stamatakis, A., Tivodar, S., Kyriacou, K., Dalezios, Y.,  Stylianopoulou, F., Karagogeos, D.  The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of Tag-1 homozygous mutants. J Neurosci. 20;30(42):13943-54 (2010).

8.    Denaxa, M., Kyriakopoulou, K., Theodorakis, K., Trichas, G., Vidaki, M., Takeda, Y., Watanabe, K., Karagogeos, D. The adhesion molecule TAG-1 is required for proper migration of the superficial migratory stream in the medulla but not of cortical interneurons. Developmental Biology, 288:87-99 (2005).

9.    Denaxa, M., Pavlou, O., Tsiotra, P., Papadopoulos, G.C., Liapaki, K., Theodorakis, K., Papadaki, C., Karagogeos, D., Papamatheakis, J. The upstream regulatory region of the gene for the human homologue of the adhesion molecule TAG-1 contains elements driving neural specific expression in vivo. Mol. Brain Res. 118:91-101, (2003).

10.    Theodorakis, K., Kyriakopoulou, K., Wassef, M. and Karagogeos, D. Novel sites of expression of the bHLH gene Nscl1 in the developing nervous system. Mechanisms of Development ,119 Suppl 1: S103-6, (2002).

11.    Pavlou, Ο., Theodorakis, Κ., Falk, J., Kutsche, M., Schachner, M., Faivre-Sarrailh, C., and Karagogeos, D. Analysis of interactions of the adhesion molecule TAG-1 and its domains with other immunoglobulin superfamily members. Molecular and Cellular Neuroscience, 20: 367-381 (2002).

12.    Tsiotra,P.C., Theodorakis,K., Papamatheakis,J., and Karagogeos,D. The fibronectin domains of the neural adhesion molecule TAX-1 are necessary and sufficient for homophilic binding. J. Biol. Chem. 271:29216-29222, (1996).

13.    Grigoriou,M., Kastrinaki,M.C., Modi,W.S., Theodorakis,K., Mankoo,B., Pachnis,V. and Karagogeos,D. Isolation of the human MOX 2 homeobox gene and localization to chromosome 7p22.1-p21.3. Genomics 26:550-555, (1995).

14.    Tsiotra,P.C., Karagogeos,D., Theodorakis,K., Michaelidis,T.M., Modi,W.S., Furley, A.J., Jessell,T.M. and Papamatheakis,J. Isolation of the cDNA and chromosomal localization of the gene (TAX 1) encoding the human axonal glycoprotein  TAG-1. Genomics 18:562-567, (1993).

15.    Kiosseoglou,V., Theodorakis,K., and Doxastakis,G. The rheology of tomato seed protein isolate films at the corn oil-water interface. Colloid Polym. Sci. 267:834-838, (1989).