Joseph Papamatheakis
Since 2014, the group has shifted research activities to focus on cancer biology. Recent advances in Molecular Biology had a dramatic impact in understanding the mechanisms that drive oncogenic pathways. In spite of that, cancer remains a major biomedical problem because of dynamic Genetic and Epigenetic heterogeneity that is the main cause of therapy resistance, disease recurrence and death. Our goals are to understand the genetic and epigenetic networks that operate and drive cancer progression and aggression. The main current directions are: 1. PML gene targets and interacting proteins. 2. Transcriptomics of cell polarity/fate protein action. 3. Tumor clonal evolution, testing of new epigenetic compounds and new gene targets.
To this ends we are using in vivo imaging, genomics, established cell lines, patient derived Glioblastoma xenografts (PDX), ectopic gene expression and genetic barcoding to study the self-renewal properties and gene and drug responses in relation to clonal and epigenetic evolution of tumor population in vivo and in vitro. These studies will contribute to the elucidation of critical oncogenic mechanisms, the development of more reliable preclinical models, the identification of predictive biomarkers and personalized therapies.
Sachini N, Arampatzi P, Klonizakis A, Nikolaou C, Makatounakis T, Lam EW, Kretsovali A, Papamatheakis J. (2019) Promyelocytic leukemia protein (PML) controls breast cancer cell proliferation by modulating Forkhead transcription factors. Mol Oncol. 13:1369-1387.
Arampatzi P, Gialitakis M, Makatounakis T, Papamatheakis J. (2013) Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements. Nucleic Acids Res. 41:2202-2215.
Gialitakis M, Arampatzi P, Makatounakis T, Papamatheakis J. (2010) Gamma Interferon dependent transcriptional memory via relocalization of a gene locus to PML nuclear bodies. Mol Cell Biol. 30:2046- 2056.
Gialitakis M, Kretsovali A, Spilianakis C, Kravariti L, Mages J, Hoffmann R, Hatzopoulos AK, Papamatheakis J. (2006) Coordinated changes of histone modifications and HDAC mobilization regulate the induction of MHC class II genes by Trichostatin A. Nucleic Acids Res. 34:765-772.
Spilianakis C, Kretsovali A, Agalioti T, Makatounakis T, Thanos D, Papamatheakis J. (2003) CIITA regulates transcription onset viaSer5-phosphorylation of RNA Pol II. EMBO J. 22:5125-5136.
Oraiopoulou M-E, E Tzamali, Joseph Papamatheakis, and Vangelis Sakkalis Phenocopying Glioblastoma: A Review. IEEE Rev Biomed Eng. 2021 Sep 10;PP. doi: 10.1109/RBME.2021.3111744.
Tampakaki M , M-E Oraiopoulou E Tzamali G Tzedakis T Makatounakis G Zacharakis J Papamatheakis and V Sakkali.s Int. J. Mol. Sci. 2021, 22, 628 https://doi.org/10.3390/ijms22126289 PML Differentially Regulates Growth and Invasion in Brain Cancer
Sachini N, A. Klonizakis, P. Arampatzi, T. Makatounakis, C. Nikolaou, E W.-F. Lam, A. Kretsovali, J. Papamatheakis. ProMyelocytic Leukemia protein IV (PMLIV) represses breast cancer proliferation by modulating the FOXO3-FOXM1 axis. Mol. Oncology. 2019 Jun; 13(6): 1369–1387
Makkou M, Takis Makatounakis and Joseph Papamathekis. A Truncated Form of the Polarity Protein SCRIB Induces Apoptosis in MDA-MB231 Cells by Regulating Components of Several Signaling Pathways. Cancer Therapy & Oncology Int. J. 14(4): CTOIJ.MS.ID.555894 (2019)
Oraiopoulou M , E. Tzamali, G. Tzedakis, E. Liapis, G. Zacharakis, A. Vakis, J. Papamatheakis & V. Sakkalis. Integrating in vitro experiments with in silico approaches for Glioblastoma invasion: the role of cell-to-cell adhesion heterogeneity Scientific Reports volume 8, Article number: 16200 (2018)43.
Sachini N, Papamatheakis J. NF-Y and the immune response: Dissecting the complex regulation of MHC genes. Biochim Biophys Acta. 2017 May;1860(5):537-542.PMID: 27989934 [PubMed - indexed for MEDLINE]
Favicchio R, Psycharakis S, Schönig K, Bartsch D, Mamalaki C, Papamatheakis J, Ripoll J, Zacharakis G. Quantitative performance characterization of three-dimensional noncontact fluorescence molecular tomography. J Biomed Opt. 21(2):26009, Feb 2016, doi: 10.1117/1.JBO.21.2.026009.PMID: 26891600 [PubMed - indexed for MEDLINE]
Hadjimichael C, Nikolaou C, Papamatheakis J, Kretsovali A. MicroRNAs for Fine-Tuning of Mouse Embryonic Stem Cell Fate Decision through Regulation of TGF-β Signaling. Stem Cell Reports. 2016 Mar 8;6(3):292-301.PMID: 26876669 [PubMed - indexed for MEDLINE]
Hadjimichael C, Chanoumidou K, Papadopoulou N, Arampatzi P, Papamatheakis J, Kretsovali A. Common stemness regulators of embryonic and cancer stem cells. World J Stem Cells. 2015 Oct 26;7(9):1150-84.PMID: 26516408 [PubMed - indexed for MEDLINE]
Hadjimichael C, Chanoumidou K, Papadopoulou N, Arampatzi P, Papamatheakis J, Kretsovali A. Promyelocytic Leukemia Protein Is an Essential Regulator of Stem Cell Pluripotency and Somatic Cell Reprogramming. Stem Cell Reports. 2017 May 9;8(5):1366-1378.PMID: 28392218 [PubMed - indexed for MEDLINE]
Stavride P, Arampatzi P, Papamatheakis J. Differential regulation of MHCII genes by PRMT6, via an AT-hook motif of RFX5. Mol Immunol. 2013 Dec;56(4):390-8.PMID: 23911394 [PubMed - indexed for MEDLINE]
Papadimitriou L, Zerva I, Georgouli M, Makatounakis T, Papamatheakis J, Athanassakis I. DOα⁻β⁺ expression in favor of HLA-DR engagement in exosomes. Immunobiology. 2013 Aug;218(8):1019-25.PMID: 23462321 [PubMed - indexed for MEDLINE]
Arampatzi P, Gialitakis M, Makatounakis T, Papamatheakis J. Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements. Nucleic Acids Res. 2013 Feb 1;41(4):2202-15. PMID: 23303784 [PubMed - indexed for MEDLINE]
Karantzali E, Lekakis V, Ioannou M, Hadjimichael C, Papamatheakis J, Kretsovali A. Sall1 Regulates Embryonic Stem Cell Differentiation in Association with Nanog. J Biol Chem. 2011 Jan 14;286(2):1037-45.PMID: 21062744 [PubMed - indexed for MEDLINE]
Gialitakis M, Arampatzi P, Makatounakis T, Papamatheakis J. Gamma Interferon-Dependent Transcriptional Memory via Relocalization of a Gene Locus to PML Nuclear Bodies. Mol Cell Biol. 2010 Apr;30(8):2046-56. PMID: 20123968 [PubMed - indexed for MEDLINE]
Garofalakis A, Zacharakis G, Meyer H, Economou EN, Mamalaki C, Papamatheakis J, Kioussis D, Ntziachristos V, Ripoll J. Three-dimensional in vivo imaging of green fluorescent protein-expressing T cells in mice with noncontact fluorescence molecular tomography. Mol Imaging. 2007 Mar-Apr;6(2):96-107.PMID: 17445504 [PubMed - indexed for MEDLINE]
Gialitakis M, Kretsovali A, Spilianakis C, Kravariti L, Mages J, Hoffmann R, Hatzopoulos AK, Papamatheakis J. Coordinated changes of histone modifications and HDAC mobilization regulate the induction of MHC class II genes by Trichostatin A. Nucleic Acids Res. 2006 Feb 1;34(3):765-72.PMID: 16452299 [PubMed - indexed for MEDLINE]
Muhlethaler-Mottet A, Krawczyk M, Masternak K, Spilianakis C, Kretsovali A, Papamatheakis J, Reith W. The S Box Of Major Histocompatibility Complex Class Ii Promoters Is A Key Determinant For Recruitment Of The Transcriptional Co-Activator Ciita. J Biol Chem. 2004 Sep 24;279(39):40529-35. PMID: 15271997 [PubMed - indexed for MEDLINE]
Biel M, Kretsovali A, Karatzali E, Papamatheakis J, Giannis A. Design, synthesis, and biological evaluation of a small-molecule inhibitor of the histone acetyltransferase Gcn5. Angew Chem Int Ed Engl. 2004 Jul 26;43(30):3974-6. No abstract available.PMID: 15274229 [PubMed - indexed for MEDLINE]
Spilianakis C, Kretsovali A, Agalioti T, Makatounakis T, Thanos D, Papamatheakis J. CIITA regulates transcription onset viaSer5-phosphorylation of RNA Pol II. EMBO J. 2003 Oct 1;22(19):5125-36. PMID: 14517250 [PubMed - indexed for MEDLINE]
Tzortzakaki E, Spilianakis C, Zika E, Kretsovali A, Papamatheakis J. Steroid receptor coactivator 1 links the steroid and interferon gamma response pathways. Mol Endocrinol. 2003 Dec;17(12):2509-18. Epub 2003 Aug 21. PMID: 12933903 [PubMed - indexed for MEDLINE]
Karetsou Z, Kretsovali A, Murphy C, Tsolas O, Papamarcaki T. Prothymosin alpha interacts with the CREB-binding protein and potentiates transcription. EMBO Rep. 2002 Apr;3(4):361-6. Epub 2002 Mar 15. PMID: 11897665 [PubMed - indexed for MEDLINE]
Kretsovali A, Spilianakis C, Dimakopoulos A, Makatounakis T, Papamatheakis J. Self-association of class II transactivator correlates with its intracellular localization and transactivation. J Biol Chem. 2001 Aug 24;276(34):32191-7. Epub 2001 Jun 18. PMID: 11413136 [PubMed - indexed for MEDLINE]
Das S, Lin JH, Papamatheakis J, Sykulev Y, Tsichlis PN. Differential splicing generates Tvl-1/RFXANK isoforms with different functions. J Biol Chem. 2002 Nov 22;277(47):45172-80. Epub 2002 Sep 4. PMID: 12215433 [PubMed - indexed for MEDLINE]
Denaxa M, Pavlou O, Tsiotra P, Papadopoulos GC, Liapaki K, Theodorakis K, Papadaki C, Karagogeos D, Papamatheakis J. The upstream regulatory region of the gene for the human homologue of the adhesion molecule TAG-1 contains elements driving neural specific expression in vivo. Brain Res Mol Brain Res. 2003 Oct 21;118(1-2):91-101. PMID: 14559358 [PubMed - indexed for MEDLINE]
Spilianakis C, Papamatheakis J, Kretsovali A. Acetylation by PCAF enhances CIITA nuclear accumulation and transactivation of major histocompatibility complex class II genes. Mol Cell Biol. 2000 Nov;20(22):8489-98. PMID: 11046145 [PubMed - indexed for MEDLINE]