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Androniki Kretsovali

Androniki Kretsovali

Principal Researcher
Email address
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Phone
+30-2810-391191
Fax
+30-2810-391101

Embryonic stem (ES) cells offer great promise for cell replacement therapies since they are highly proliferating cells that are able to self-renew and retain the capacity to differentiate along almost all possible differentiation pathways. Our group is interested in the genetic and epigenetic regulation of ES cell pluripotency. We study the role of transcriptional regulators and micro-RNAs in: 1. stem cell differentiation and 2. reprogramming of somatic cells into pluripotency. For this aim we are using gene silencing and overexpression techniques as well as genome-wide (genomic and proteomic) methodologies. In addition, we analyse the importance of epigenetic regulatory mechanisms such as histone modifications in cell specification decisions. For this purpose we exploit the effect of small molecules and employ novel biomaterial substrates as scaffolds for selective cellular growth and/or differentiation.

Hadjimichael C, Chanoumidou K, Nikolaou C, Klonizakis A, Theodosi GI, Makatounakis T, Papamatheakis J, Kretsovali A. (2017) Promyelocytic Leukemia Protein Is an Essential Regulator of Stem Cell Pluripotency and Somatic Cell Reprogramming. Stem Cell Reports 8:1366-1378.

Hadjimichael C, Nikolaou C, Papamatheakis J, Kretsovali A. (2016) MicroRNAs for Fine-Tuning of Mouse Embryonic Stem Cell Fate Decision through Regulation of TGF-β Signaling. Stem Cell Reports 6:292-301.

Karantzali E, Lekakis V, Ioannou M, Hadjimichael C, Papamatheakis J, Kretsovali A. (2011) Sall1 regulates embryonic stem cell differentiation in association with Nanog. J Biol Chem. 286:1037-45.

Schulz H, Kolde R... Karantzali E, Kretsovali A, Hatzopoulos AK. (2009) The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation. PLoS One 4:e6804.

Karantzali E, Schulz H, Hummel O, Hubner N, Hatzopoulos A.K., Kretsovali A. (2008) Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis. Genome Biology 9:R65.

 

 

Hadjimichael C, Chanoumidou K, Nikolaou C, Klonizakis A, Theodosi GI, Makatounakis T, Papamatheakis J, Kretsovali A.
Promyelocytic Leukemia Protein Is an Essential Regulator of Stem Cell Pluripotency and Somatic Cell Reprogramming. Stem Cell Reports. 2017 May 9;8(5):1366-1378

Hadjimichael C, Nikolaou C, Papamatheakis J, Kretsovali A.
MicroRNAs for Fine-Tuning of Mouse Embryonic Stem Cell Fate Decision through Regulation of TGF-β Signaling. Stem Cell Reports. 2016 Mar 8;6(3):292-301. doi: 10.1016/j.stemcr.2016.01.004. Epub 2016 Feb 11

Hadjimichael C, Chanoumidou K, Papadopoulou N, Arampatzi P, Papamatheakis J, Kretsovali A.
Common stemness regulators of embryonic and cancer stem cells.World J Stem Cells. 2015 Oct 26;7(9):1150-84

Kretsovali A, Hadjimichael C, Charmpilas N.
Histone deacetylase inhibitors in cell pluripotency, differentiation, and reprogramming. Stem Cells Int. 2012, 2012:184154

Karantzali E, Lekakis V, Ioannou M, Hadjimichael C, Papamatheakis J, Kretsovali A.
Sall1 regulates embryonic stem cell differentiation in association with Nanog. J.Biol. Chem. 2011 Jan 14;286(2):1037-45

Schulz H, Kolde R, Adler P, Aksoy I, Anastassiadis K, Bader M, Billon N, Boeuf H, Bourillot PY, Buchholz F, Dani C, Doss MX, Forrester L, Gitton M, Henrique D, Hescheler J, Himmelbauer H, Hübner N, Karantzali E, Kretsovali A, Lubitz S, Pradier L, Rai M, Reimand J, Rolletschek A, Sachinidis A, Savatier P, Stewart F, Storm MP, Trouillas M, Vilo J, Welham MJ, Winkler J, Wobus AM, Hatzopoulos AK; Functional Genomics in Embryonic Stem Cells Consortium.
The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation.
PLoS One. 2009 Sep 3;4(9):e6804.

Karantzal E, Schulz H, Hummel O, Hubner N, Hatzopoulos A, Kretsovali A.
Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis. Genome Biol. 2008 Apr 4; 9 ( 4 ): R65 . [Epub ahead of print] PMID: 18394158 [PubMed - as supplied by publisher]

Gialitakis M, Kretsovali A, Spilianakis C, Kravariti L, Mages J, Hoffmann R, Hatzopoulos AK, Papamatheakis J.
Coordinated changes of histone modifications and HDAC mobilization regulate the induction of MHC class II genes by Trichostatin A. Nucleic Acids Res. 2006 Feb 1; 34 ( 3 ): 765-72. Print 2006. PMID: 16452299 [PubMed - indexed for MEDLINE]

Biel M, Kretsovali A, Karatzali E, Papamatheakis J, Giannis A.
Design, synthesis, and biological evaluation of a small-molecule inhibitor of the histone acetyltransferase Gcn5.
Angew Chem Int Ed Engl. 2004 Jul 26;43(30):3974-6. No abstract available.
PMID: 15274229 [PubMed - indexed for MEDLINE]

Muhlethaler-Mottet A, Krawczyk M, Masternak K, Spilianakis C, Kretsovali A, Papamatheakis J, Reith W.
The S box of major histocompatibility complex class II promoters is a key determinant for recruitment of the transcriptional co-activator CIITA.
J Biol Chem. 2004 Sep 24;279(39):40529-35. Epub 2004 Jul 22.
PMID: 15271997 [PubMed - indexed for MEDLINE]

Spilianakis C, Kretsovali A, Agalioti T, Makatounakis T, Thanos D, Papamatheakis J.

CIITA regulates transcription onset viaSer5-phosphorylation of RNA Pol II.
EMBO J. 2003 Oct 1;22(19):5125-36.
PMID: 14517250 [PubMed - indexed for MEDLINE]

Tzortzakaki E, Spilianakis C, Zika E, Kretsovali A, Papamatheakis J.

Steroid receptor coactivator 1 links the steroid and interferon gamma response pathways.
Mol Endocrinol. 2003 Dec;17(12):2509-18. Epub 2003 Aug 21.
PMID: 12933903 [PubMed - indexed for MEDLINE]

Mamalaki A, Gritzapis AD, Kretsovali A, Belimezi M, Papamatheakis J, Perez SA, Papamichail M, Baxevanis CN.
In vitro and in vivo antitumor activity of a mouse CTL hybridoma expressing chimeric receptors bearing the single chain Fv from HER-2/neu- specific antibody and the gamma-chain from Fc(epsilon) RI.
Cancer Immunol Immunother. 2003 Aug;52(8):513-22. Epub 2003 Apr 25.
PMID: 12715240 [PubMed - indexed for MEDLINE]

Gritzapis AD, Mamalaki A, Kretsovali A, Papamatheakis J, Belimezi M, Perez SA, Baxevanis CN, Papamichail M.
Redirecting mouse T hybridoma against human breast and ovarian carcinomas: in vivo activity against HER-2/neu expressing cancer cells.
Br J Cancer. 2003 Apr 22;88(8):1292-300.
PMID: 12698199 [PubMed - indexed for MEDLINE]

Karetsou Z, Kretsovali A, Murphy C, Tsolas O, Papamarcaki T.
Prothymosin alpha interacts with the CREB-binding protein and potentiates transcription.
EMBO Rep. 2002 Apr;3(4):361-6. Epub 2002 Mar 15.
PMID: 11897665 [PubMed - indexed for MEDLINE]

Kretsovali A, Spilianakis C, Dimakopoulos A, Makatounakis T, Papamatheakis J.

Self-association of class II transactivator correlates with its intracellular localization and transactivation.
J Biol Chem. 2001 Aug 24;276(34):32191-7. Epub 2001 Jun 18.
PMID: 11413136 [PubMed - indexed for MEDLINE]

Spilianakis C, Papamatheakis J, Kretsovali A.
Acetylation by PCAF enhances CIITA nuclear accumulation and transactivation of major histocompatibility complex class II genes.
Mol Cell Biol. 2000 Nov;20(22):8489-98.
PMID: 11046145 [PubMed - indexed for MEDLINE]