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Inga Siden-Kiamos

Inga Siden-Kiamos

Principal Researcher
Email address
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Phone
+30-2810-391118
Fax
+30-2810-391104

Malaria is a disease having a huge impact on human health and economic development with about half a million lethal cases every year. It is primarily a disease of less developed countries in Africa and South East Asia. Malaria is controlled by drugs targeting the parasite in the human host and insecticides to limit the exposure to the mosquito. However, resistance of the parasite to the drugs used and of the mosquitoes to the insecticides threaten these strategies. No vaccine is currently available.

Malaria is caused by unicellular protozoan parasites of the genus Plasmodium, which are transmitted by anopheline mosquitoes. It is recognized that in order to eliminate or even eradicate the disease, methods to block transmission through the mosquito are crucial prerequisites. However, the development of such methods is hampered by the fact that these parasite stages are not well understood.

The research in our laboratory centers on understanding key events during the development of the parasite within the mosquito using a combination of techniques such as reverse genetics together with cell and molecular biology. Our own expertise is complemented with that of collaborating groups around the world. 

 

Andreadaki M, Hanssen E, Deligianni E, Claudet C, Wengelnik K, Mollard V, McFadden GI, Abkarian M, Braun-Breton C, Siden-Kiamos I. (2018) Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood. Cell. Sci. Rep. 8:3543.

Kefi M, Mavridis K, Simões ML, Dimopoulos G, Siden-Kiamos I, Vontas J. (2018) New rapid one-step PCR diagnostic assay for Plasmodium falciparum infective mosquitoes. Sci. Rep. 8:1462.

Currà C, Gessmann R, Pace T, Picci L, Peruzzi G, Varamogianni-Mamatsi V, Spanos L, Garcia CR, Spaccapelo R, Ponzi M, Siden-Kiamos I (2016) Release of Plasmodium sporozoites requires proteins with histone-fold dimerization domains. Nat Commun. 7:13846.

Vahokoski J, Bhargav S.P, Desfosses A, Andreadaki M, Kumpula E.-P, Martinez S.M, Ignatev A, Lepper S, Frischknecht F, Sidén-Kiamos I, Sachse C, Kursula I. (2014) Structural Differences Explain Diverse Functions of Plasmodium Actins. PLoS Pathog. 10:e1004091.

Andreadaki M, Morgan RN, Deligianni E, Kooij TW, Santos JM, Spanos L, Matuschewski K, Louis C, Mair GR, Siden-Kiamos I. (2014) Genetic crosses and complementation reveal essential functions for the Plasmodium stage-specific actin2 in sporogonic development. Cell Microbiol. PMID: 24471657.

 

2018

Siden-Kiamos I, Pace T, Klonizakis A, Nardini M, Garcia CRS, Currà C. (2018) Identification of Plasmodium berghei Oocyst Rupture Protein 2 (ORP2) domains involved in sporozoite egress from the oocyst. Int J Parasitol. 2018 Dec;48(14):1127-1136. doi: 10.1016/j.ijpara.2018.09.004.
Pubmed

Martí Coma-Cros E, Biosca A, Marques J, Carol L, Urbán P, Berenguer D, Riera MC, Delves M, Sinden RE, Valle-Delgado JJ, Spanos L, Siden-Kiamos I, Pérez P, Paaijmans K, Rottmann M, Manfredi A, Ferruti P, Ranucci E, Fernàndez-Busquets X. (2018) Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs. Pharmaceutics. 2018 Nov 10;10(4). pii: E225. doi: 10.3390/pharmaceutics10040225.
Pubmed

Deligianni E, Silmon de Monerri NC, McMillan PJ, Bertuccini L, Superti F, Manola M, Spanos L, Louis C, Blackman MJ, Tilley L, Siden-Kiamos I. (2018) Essential role of Plasmodium perforin-like protein 4 in ookinete midgut passage. PLoS One. 2018 Aug 13;13(8):e0201651. doi: 10.1371/journal.pone.0201651.
Pubmed

Goulielmaki E, Kaforou S, Venugopal K, Loukeris TG, Siden-Kiamos I, Koussis K. (2018) Distinct effects of HIV protease inhibitors and ERAD inhibitors on zygote to ookinete transition of the malaria parasite. Mol Biochem Parasitol 220, March 2018, Pages 10-14.
Pubmed

Kefi M, Mavridis K, Simões ML, Dimopoulos G, Siden-Kiamos I, Vontas J. (2018) New rapid one-step PCR diagnostic assay for Plasmodium falciparum infective mosquitoes. Sci. Rep. 23;8(1):1462. doi: 10.1038/s41598-018-19780-6.
Pubmed

Deligianni E, Andreadaki M, Koutsouris K, Siden-Kiamos I. (2018) Sequence and functional divergence of gametocyte-specific parasitophorous vacuole membrane proteins in Plasmodium parasites. Mol Biochem Parasitol 10.1016/j.molbiopara.2018.01.002.
Pubmed

Andreadaki M, Hanssen E, Deligianni E, Claudet C, Wengelnik K, Mollard V, McFadden GI, Abkarian M, Braun-Breton C, Siden-Kiamos I. (2018) Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell. Sci. Rep. Feb 23;8(1):3543. doi: 10.1038/s41598-018-21801-3.
Pubmed

2017

Koussis K, Goulielmaki E, Chalari A, Withers-Martinez C, Siden-Kiamos I, Matuschewski K, Loukeris TG. (2017) Targeted Deletion of a Plasmodium Site-2 Protease Impairs Life Cycle Progression in the Mammalian Host. PLoS One. 12(1):e0170260
Pubmed

2016

Currà C, Gessmann R, Pace T, Picci L, Peruzzi G, Varamogianni-Mamatsi V, Spanos L, Garcia CR, Spaccapelo R, Ponzi M, Siden-Kiamos I (2016) Release of Plasmodium sporozoites requires proteins with histone-fold dimerization domains. Nat Commun. 2016 Dec 16;7:13846. doi: 10.1038/ncomms13846.
Pubmed

Andreadaki M, Deligianni E, Nika F, Siden-Kiamos I. (2016) Expression of the Plasmodium berghei actin II gene is controlled by elements in a long genomic region. Parasitol Res. 115(8):3261-5. 
Pubmed

2015

Andreadaki M, Mollenkopf HJ, Nika F, Brady D, Tewari R, Matuschewski K, Siden-Kiamos I. (2015) Global expression profiling reveals shared and distinct transcript signatures in arrested act2(-) and CDPK4(-) Plasmodium berghei gametocytes.Mol Biochem Parasitol.201(2):100-7.
Pubmed

Tampaki Z, Mwakubambanya RS, Goulielmaki E, Kaforou S, Kim K, Waters AP, Carruthers VB, Siden-Kiamos I, Loukeris TG, Koussis K. (2015) Ectopic Expression of a Neospora caninum Kazal Type Inhibitor Triggers Developmental Defects in Toxoplasma and Plasmodium. PLoS One. 2015 Mar 24;10(3):e0121379. doi: 10.1371/journal.pone.0121379
Pubmed

Olivieri A, Bertuccini L, Deligianni E, Franke-Fayard B, Currà C, Siden-Kiamos I, Hanssen E, Grasso F, Superti F, Pace T, Fratini F, Janse CJ, Ponzi M. (2015) Distinct properties of the egress-related osmiophilic bodies in male and female gametocytes of the rodent malaria parasite Plasmodium berghei. Cell Microbiol. 2015 Mar; 17(3):355-68. doi: 10.1111/cmi.12370
Pubmed

Hliscs M, Millet C, Dixon MW, Siden-Kiamos I, McMillan P, Tilley L. (2015) Organisation and function of an actin cytoskeleton in Plasmodium falciparum gametocytes. Cell Microbiol. 2015 Feb;17(2):207-25. doi: 10.1111/cmi.12359
Pubmed

2014

Guerreiro A, Deligianni E, Santos J. M, Silva P, Louis C, Pain A, Janse C. J, Franke-Fayard B, Carret C. K, Sidén-Kiamos I, Mair G R. (2014) Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte. Genome Biology 15:493
Pubmed

Goulielmaki E, Sidén-Kiamos I, Loukeris TG. (2014) Functional Characterization of Anopheles Matrix Metalloprotease 1 Reveals Its Agonistic Role during Sporogonic Development of Malaria Parasites. Infect Immun. 2014 Nov;82(11):4865-77. doi: 10.1128/IAI.02080-14
Pubmed

Andreadaki, M., Morgan, R.N., Deligianni, E., Kooij T.W.A., Santos, J.M., Spanos, L., Matuschewski, K., Louis, C., Mair, G.R., and Siden-Kiamos, I. (2014) Genetic crosses and complementation reveal essential functions for the Plasmodium stage-specific actin2 in sporogonic development. Cell. Microbiol. 16(5):751-67. doi: 10.1111/cmi.12274
Pubmed

Vahokoski, J., Bhargav, S.P., Desfosses, A., Andreadaki, M., Kumpula, E.-P., Martinez, S.M., Ignatev, A., Lepper, S., Frischknecht, F., Sidén-Kiamos, I., Sachse, C., and Kursula, I. (2014) Structural Differences Explain Diverse Functions of Plasmodium Actins. PLoS Pathog. Apr 17;10(4):e1004091. doi: 10.1371/journal.ppat.1004091.
Pubmed

2013

Deligianni, E., Morgan, R.N., Bertuccini, L., Wirth, C.C., Silmon de Monerri, N.C., Spanos, L., Blackman, M.J., Louis , C., Pradel, G., and Siden-Kiamos, I. (2013) A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes Cell. Microbiol. 15(8):1438-55, Aug 2013
Pubmed

Currà C, Di Luca M, Picci L, de Sousa Silva Gomes dos Santos C, Siden-Kiamos I, Pace T, Ponzi M.  (2013) The ETRAMP family member SEP2 is expressed throughout Plasmodium berghei life cycle and is released during sporozoite gliding motility. PLOS One 8(6):e67238
Pubmed

2012

Siden-Kiamos, I., Louis, C., Matuschewski, K. (2012). Evidence for filamentous actin in ookinetes of a malarial parasite. Mol. Biochem. Parasitol. 181(2):186-9
Pubmed

2011

Deligianni E, Morgan RN, Bertuccini L, Kooij TW, Laforge A, Nahar C, Poulakakis N, Schüler H, Louis C, Matuschewski K, Siden-Kiamos I. (2011) Critical role for a stage-specific actin in male exflagellation of the malaria parasite. Cell Microbiol. 13:1714-1730
Pubmed

Siden-Kiamos I, Ganter M, Kunze A, Hliscs M, Steinbüchel M, Mendoza J, Sinden RE, Louis C, Matuschewski, K. (2011) Stage-specific Depletion of Myosin A Supports an Essential Role in Motility of Malarial Ookinetes. Cell Microbiol.13:1996-2006
Pubmed

2010

Siden-Kiamos I, Schüler H, Liakopoulos D, Louis C. (2010) Arp1, an actin-related protein, in Plasmodium berghei. Mol Biochem Parasitol. 173(2):88-96
Pubmed

2009

Ponzi M, Sidén-Kiamos I, Bertuccini L, Currà C, Kroeze H, Camarda G, Pace T, Franke-Fayard B, Laurentino EC, Louis C, Waters AP, Janse CJ, Alano P. (2009) Egress of Plasmodium berghei gametes from their host erythrocyte is mediated by the MDV-1/PEG3 protein. Cell Microbiol. 11(8):1272-88
Pubmed