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Vassiliki Nikoletopoulou

Vassiliki Nikoletopoulou

IMBB Group Leader (Assistant Professor Grade)
Email address
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Phone
+30-2810-391988
Fax
+30-2810-391101

The main interest of our group is to investigate the process of autophagic degradation in mammalian neurons. Autophagy is a highly conserved catabolic pathway that degrades macromolecules as well as damaged or superfluous organelles by delivering them to the lysosome. Baseline autophagy is crucial for neuronal integrity and its deregulation causes severe synaptic defects and autistic behaviors. Despite this, the regulation of autophagy in neurons remains elusive. Our research aims to characterize the molecular mechanisms that regulate autophagy in the mammalian brain and to reveal the functional roles and cargo of autophagy in neurons and synapses. To this end, we employ genetic tools, such as conditional mouse knockouts and transgenic mouse reporters, along with biochemical and cell biology techniques.

Pediaditakis I, Efstathopoulos P, Prousis KC, Zervou M, Arévalo JC, Alexaki VI, Nikoletopoulou V, Karagianni E, Potamitis C, Tavernarakis N, Chavakis T, Margioris AN, Venihaki M, Calogeropoulou T, Charalampopoulos I, Gravanis A. (2016) Selective and differential interactions of BNN27, a novel C17-spiroepoxy steroid derivative, with TrkA receptors, regulating neuronal survival and differentiation. Neuropharmacology 111:266-282.

Kourtis N, Nikoletopoulou V, Tavernarakis N. (2012) Small heat shock proteins protect from heat stroke-associated neurodegeneration. Nature 490:213-8.

Tiwari VK, Burger L, Nikoletopoulou V, Deogracias R, Thakurela S, Wirbelauer C, Kaut J, Terranova R, Hoerner L, Mielke C, Boege F, Murr R, Peters AH, Barde YA, Schübeler D. (2012) Target genes of Topoisomerase IIβ regulate neuronal survival and are defined by their chromatin state. Proc Natl Acad Sci U S A 109:E934-43.

Nikoletopoulou V, Lickert H, Frade JM, Rencurel C, Giallonardo P, Zhang L, Bibel M, Barde YA. (2010) Neurotrophin receptors TrkA and TrkC cause neuronal death whereas TrkB does not. Nature 467:59-63.

Nikoletopoulou V, Plachta N, Allen ND, Pinto L, Götz M, Barde YA. (2007) Neurotrophin receptor-mediated death of misspecified neurons generated from embryonic stem cells lacking Pax6. Cell Stem Cell 1:529-40.

Articles

1. Vanderluit, J. L., Ferguson, K. L., Nikoletopoulou, V., Parker, M., Ruzhynsky, V., Alexson, T., McNamara, S. M., Park, D. S., Rudnicki, M., Slack, R. S. (2004). p107 regulates neural precursor cells in the mammalian brain. J. Cell Biol. 166:853-863.

2. Nikoletopoulou, V., Plachta, N., Allen, N.D:, Pinto L., Götz, M. and Barde, Y.-A. (2007) Neurotrophin receptor-mediated death of misspecified neurons generated from embryonic stem cells lacking Pax6. Cell Stem Cell 1, 529-540.

3. Nikoletopoulou, V., Frade, J.-M., Rencurel, C., Lickert, H., Giallonardo, P., Bibel, M., Barde, Y.-A. (2010) Neurotrophin receptors TrkA and TrkC cause neuronal death whereas TrkB does not. Nature 467, 59-63.

4. Tiwari, V.K.*, Burger, L.*, Nikoletopoulou, V.*, Deogracias, R., Thakurela, S., Wirbelauer, C., Kaut, J., Terranova, R., Hoerner, L., Mielke, C., Boege, F., Murr, R., Peters, A.H., Barde, Y.A., Schuebeler, D. (2012) Target genes of Topoisomerase IIb regulate neuronal survival and are defined by their chromatin state. PNAS 109, 934-43.

5. Kourtis, N., Nikoletopoulou, V., and Tavernarakis, N. (2012) Small heat shock proteins protect from heat stroke-associated neurodegeneration. Nature 490(7419):213-8.

6. Pediaditakis, I., Efstathopoulos, P., Prousis, K.C., Zervou, M., Arevalo, J.C., Alexaki, V.I., Nikoletopoulou, V., Karagianni E., Potamitis C., Tavernarakis N., Chavakis T., Margioris A.N., Venihaki M., Calogeropoulou T., Charalampopoulos I., Gravanis A. (2016) Selective and differential interactions of BNN27, a novel C17-spiroepoxy steroid derivative, with TrkA receptors, regulating neuronal survival and differentiation. Neuropharmacology 111:266-282.

7. Nikoletopoulou, V., Sidiropoulou, K. and Tavernarakis, N. (2017) Modulation of autophagy by BDNF underlies synaptic plasticity. Under revision in Cell Metabolism

Reviews

1. Bischoff,V., Nikoletopoulou, V., Barde, Y.A (2011) Save or kill- the ambivalence of neurotrophin receptors. Med. Sci.(Paris) 2, 119-21.

2. Dekkers, M., Nikoletopoulou, V. and Barde, Y. A. (2013) Death of developing neurons: new insights and implications for connectivity. JCB 203(3):385-93.

3. Nikoletopoulou, V. and Tavernarakis, N. (2012) Embryonic and induced pluripotent stem cell differentiation as a tool in neurobiology. Biotechnology journal 7(9):115668.

4. Nikoletopoulou, V. and Tavernarakis, N. (2012) Calcium homeostasis in aging neurons. Frontiers in Genetics, PMID:23060904.

5. Kourtis, N., Nikoletopoulou, V., Tavernarakis, N. (2012) Heat shock response and ionstasis: axis against neurodegeneration. Aging 4(12):856-8.

6. Nikoletopoulou, V. and Tavernarakis, N. (2013) Mitochondrial Biogenesis and Dynamics in Neurodegeneration: A Causative Relationship. Neurochem Res. [Epub ahead of print]

7. Nikoletopoulou, V., Markaki, M., Palikaras, K., Tavernarakis N. (2013) Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta. [Epub ahead of print].

8. Nikoletopoulou, V., Kyriakakis, E. and Tavernarakis, N. (2013). Cellular and molecular longevity pathways: the old and the new. Trends Endocrinology Metabolism, PMID:24388148

9. Nikoletopoulou, V., Papandreou, M.E. and Tavernarakis, N. (2014). Autophagy in neuronal physiology and pathology. Cell death and Differentiation.

Book chapters

Nikoletopoulou, V and Tavernarakis, N. (2014). Necrotic cell death in C. elegans. Methods in Enzymology. PMID: 25065889