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Anastasios (Tassos) Pavlopoulos

Anastasios (Tassos) Pavlopoulos

IMBB Group Leader (Assistant Professor Grade)
Email address
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Phone
+30-2810-391178
Fax
+30-2810-391101

We are the Developmental Morphogenesis Lab in Crete studying the origin of biological form during animal development and evolution. Our research aims to understand the molecular, cellular and biophysical mechanisms that sculpt different tissue shapes and sizes to suit the lifestyle of the organisms. To achieve this goal, we are focusing on genetically and optically tractable arthropod species that satisfy a number of appealing biological and technical requirements. We combine advanced microscopy and image analysis pipelines with genetic and mechanical perturbations to reconstruct and analyse intact developing embryos from a bottom-up cellular perspective, and to understand how the genomic information is translated into morphogenetic cellular activities like patterned cell proliferation, change of shape and movement.

Münster S, Jain A, Mietke A, Pavlopoulos A, Grill SW, Tomancak P. (2019) Attachment of the blastoderm to the vitelline envelope affects gastrulation of insects. Nature 568:395-399.

Wolff C, Tinevez JY, Pietzsch T, Stamataki E, Harich B, Guignard L, Preibisch S, Shorte S, Keller PJ, Tomancak P, Pavlopoulos A. (2018) Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb. Elife 7:e34410.

Kao D, Lai AG, Stamataki E, Rosic S, Konstantinides N, Jarvis E, Di Donfrancesco A, Pouchkina-Stancheva N, Semon M, Grillo M, Bruce H, Kumar S, Siwanowicz I, Le A, Lemire A, Eisen MB, Extavour C, Browne WE, Wolff C, Averof M, Patel NH, Sarkies P, Pavlopoulos A, Aboobaker A. (2016) The genome of the crustacean Parhyale hawaiensis, a model for animal development, regeneration, immunity and lignocellulose digestion. Elife 5:e20062.

Benton MA, Pechmann M, Frey N, Stappert D, Conrads KH, Chen YT, Stamataki E, Pavlopoulos A, Roth S. (2016) Toll genes have an ancestral role in axis elongation. Curr Biol. 26:1609-1615.

Benton MA, Akam M, Pavlopoulos A. (2013) Cell and tissue dynamics during Tribolium castaneum embryogenesis revealed by versatile fluorescence labeling approaches. Development 140:3210-3220.

Jain A, Ulman V, Mukherjee A, Prakash M, Pimpale L, Münster S, Panfilio KA, Jug F, Grill SW, Tomancak P, Pavlopoulos A. (2019) Regionalized tissue fluidization by an actomyosin cable is required for epithelial gap closure during insect gastrulation. bioRxiv:744193.

Münster S, Jain A, Mietke A, Pavlopoulos A, Grill SW, Tomancak P. (2019) Attachment of the blastoderm to the vitelline envelope affects gastrulation of insects. Nature 568:395-399.

Pavlopoulos A, Wolff C. (2019) Crustacean limb morphogenesis during normal development and regeneration. The Natural History of the Crustacea Volume VII - Developmental Biology and Larval Ecology.

Wolff C, Tinevez JY, Pietzsch T, Stamataki E, Harich B, Guignard L, Preibisch S, Shorte S, Keller PJ, Tomancak P, Pavlopoulos A. (2018) Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb. Elife 7:e34410.

Stamataki E, Pavlopoulos A. (2016) Non-insect crustacean models in developmental genetics including an encomium to Parhyale hawaiensis. Current Opinion in Genetics & Development 39:149-156.

Kao D, Lai AG, Stamataki E, Rosic S, Konstantinides N, Jarvis E, Di Donfrancesco A, Pouchkina-Stancheva N, Semon M, Grillo M, Bruce H, Kumar S, Siwanowicz I, Le A, Lemire A, Eisen MB, Extavour C, Browne WE, Wolff C, Averof M, Patel NH, Sarkies P, Pavlopoulos A, Aboobaker A. (2016) The genome of the crustacean Parhyale hawaiensis, a model for animal development, regeneration, immunity and lignocellulose digestion. Elife 5:e20062.

Benton MA, Pechmann M, Frey N, Stappert D, Conrads KH, Chen YT, Stamataki E, Pavlopoulos A, Roth S. (2016) Toll genes have an ancestral role in axis elongation. Current Biology 26:1609-1615.

Benton MA, Pavlopoulos A. (2014) Tribolium embryo morphogenesis: May the force be with you. Bioarchitecture 4:16-21.

Kontarakis Z, Pavlopoulos A. (2014) Transgenesis in non-model organisms: the case of Parhyale, Hox genes: Methods and Protocols. Methods in Molecular Biology 1196:145-181.

Benton MA, Akam M, Pavlopoulos A. (2013) Cell and tissue dynamics during Tribolium castaneum embryogenesis revealed by versatile fluorescence labeling approaches. Development 140:3210-3220.

Kontarakis Z, Konstantinides N, Pavlopoulos A, Averof M. (2011) Reconfiguring gene traps for new tasks using iTRAC. Fly 5:352-355.

Kontarakis Z, Pavlopoulos A, Kiupakis A, Konstantinides N, Douris V, Averof M. (2011) A versatile strategy for gene trapping and trap conversion in emerging model organisms. Development 138:2625-2630.

Pavlopoulos A. (2011) Identification of DNA sequences that flank a known region by inverse PCR, Molecular Methods for Evolutionary Genetics. Methods in Molecular Biology 772:267-275.

Pavlopoulos A, Akam M. (2011) Hox gene Ultrabithorax regulates distinct sets of target genes at successive stages of Drosophila haltere morphogenesis. Proceedings of the National Academy of Sciences USA 108:2855-2860.

Averof M, Pavlopoulos A, Kontarakis Z. (2010) Evolution of new appendage types by gradual changes in Hox gene expression – the case of crustacean maxillipeds. Palaeodiversity Suppl 3:141-146.

Pavlopoulos A, Kontarakis Z, Liubicich DM, Serano JM, Akam M, Patel NH, Averof M. (2009) Probing the evolution of appendage specialization by Hox gene misexpression in an emerging model crustacean. Proceedings of the National Academy of Sciences USA 106:13897-13902.

Liubicich DM, Serano JM, Pavlopoulos A, Kontarakis Z, Protas ME, Kwan E, Chatterjee S, Tran KD, Averof M, Patel NH (2009) Knockdown of Parhyale Ultrabithorax recapitulates evolutionary changes in crustacean appendage morphology. Proceedings of the National Academy of Sciences USA 106:13892-13896.

Pavlopoulos A, Oehler S, Kapetanaki MG, Savakis C. (2007) The DNA transposon Minos as a tool for transgenesis and functional genomic analysis in vertebrates and invertebrates. Genome Biology 8 Suppl 1:S2.

Pavlopoulos A, Akam M. (2007) Hox go omics: insights from Drosophila into Hox gene targets. Genome Biology 8:208.

Pavlopoulos A, Averof M. (2005) Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis. Proceedings of the National Academy of Sciences USA 102:7888-7893.

Pavlopoulos A, Berghammer AJ, Averof M, Klingler M. (2004) Efficient transformation of the beetle Tribolium castaneum using the Minos transposable element: quantitative and qualitative analysis of genomic integration events. Genetics 167:737-746.

Pavlopoulos A, Averof M. (2002) Developmental evolution: Hox proteins ring the changes. Current Biology 12:R291-293.